免疫测定

The cardiomyocyte viability were detected by MTT assay for calculating the survival rate and the inhibitory rate of the virus. Myocardial F-actin and VEGF were analyzed by immunofluorescent staining with confocal microscopy. Mean fluorescent intensity of F-actin and VEGF were determined by flow cytometry.Results There is no toxicity on cardiomyocytes when PD is at concentration of 0.02 mmol/L and 0.2 mmol/L while there is toxicity at concentration of 2 mmol/L. CVB3 could reduce the viability of cardiomyocytes, depolymerize F-actin cytoskeleton and reorganize VEGF protein.

体外培养心肌细胞，用100 TCID50柯萨奇B3病毒（CVB3）感染心肌细胞，建立病毒性心肌炎实验模型，然后分别用0.02mmol/L、0.2mmol/L、2mmol/L三种不同浓度的PD处理感染CVB3病毒的心肌细胞，观察心肌细胞的形态和搏动，用细胞免疫荧光技术标记纤维状肌动蛋白和VEGF蛋白，四唑蓝比色法测定心肌细胞活性和病毒抑制率，流式细胞仪检测各组心肌细胞VEGF蛋白和F-actin平均荧光强度。

The model of thyroid hormone-induced diarrheic rats was prepared and interfered by low, medium and high dose of Asi-antidiarrheal capsule and berberine. Immunohistochemistry was used to detect the population, ratio of degranulation, area, perimeter, equivalent diameter and cyclo-dimension of the MC in duodenum mucosa of control group, model group, berberine group and Asi-antidiarrheal capsule group.

运用阿是止泻胶囊低、中、高剂量及黄连素对其干预，采用免疫组化技术测定对照组、模型组、黄连素组、阿是止泻胶囊低、中、高剂量组大鼠十二指肠黏膜MC数量、脱颗粒比率、面积、周长、等效直径及圆形度。

Methods HA and TGFβ1 were measured concurrently in synovial fluid samples from 23 patients with mild OA, 28 patients with severe OA and 6 patients with disciform meniscus. The determinations of positive rate of cellsin genual synovium with HA and TGFβ1 were detected by using immunohistochemical techonology.

提取23例OA病变轻微患者，28例病变严重患者，6例对照者的滑膜组织及关节液，采用免疫组织化学方法检测膝关节滑膜组织中HA、TGFβ1的阳性细胞率及通过夹心ELISA法测定膝关节滑液中HA、TGFβ1含量。

Methods IL-1and MMP-13were measured concurrently in synovial fluid samples from23patients with mild OA,28patients with severe OA and6patients with disciform meniscus.The determinations of positive rate of cells in genual synovium with IL-1and MMP-13were carried out using immunohistochemical techonology.

提取23例OA病变轻微患者、28例OA病变严重患者、6例对照者的滑膜组织及关节液，采用免疫组织化学方法检测膝关节滑膜组织中IL-1、MMP-13的阳性细胞率及通过夹心ELISA法测定膝关节滑液中IL-1、MMP-13含量。

Objective To study the relationship between IL-1,MMP-13and pathogenesis of knee osˉteoarthrosis.Methods IL-1and MMP-13were measured concurrently in synovial fluid samples from23patients with mild OA,28patients with severe OA and6patients with disciform meniscus.The determinations of positive rate of cells in genual synovium with IL-1and MMP-13were carried out using immunohistochemical techonology.

目的 探讨白细胞介素-1（IL-1）、基质金属蛋白酶-13（MMP-13）在膝关节骨性关节炎发病中的作用方法提取23例OA病变轻微患者、28例OA病变严重患者、6例对照者的滑膜组织及关节液，采用免疫组织化学方法检测膝关节滑膜组织中IL-1、MMP-13的阳性细胞率及通过夹心ELISA法测定膝关节滑液中IL-1、MMP-13含量。

In this research,taking Wistar rats models of SCI made by Allen"s as objects of study ,treatment is taken by Jia Ji electropuncture and dexamenthasomein 6h and 12h after SCIin T_(11_12) segments ;model rat"s motor and sensory function are studied BDNF ,NGF,BDNFmRNA ,BCL-2,BCL-2mRNA,Bax are studiedbyimmunohistochemistry ,insituhybridization ,optic ,electro n microscope in different time .

本课题以Wistar大鼠经Allen's法制备脊髓撞击伤模型，对胸11～12损伤节段采取不同时间进行夹脊电针治疗，即伤后6h和12h接受夹脊电针治疗和地塞米松治疗，以联合行为评分法测定不同时间损伤大鼠的运动功能，感觉功能，以免疫组化法，原位杂交法，光镜技术，电镜技术，观察不同时间点伤段脊髓的BDNF，BDNFmRNA，NGF，BCL-2，BCL-2mRNA，BAX表达。

Methods The levels of serum CRP in 210 patients with malignant tumor and 45 healthy persons were measured by emulsive immunodiffusion.They were analyzed by statistical method.

采用免疫散射比浊法测定210例不同类型恶性肿瘤患者及45例健康体检者的血清CRP水平并进行比较分析。

Based on pre-experiment foundation of the earlier period, 60 male patients among 45-65 year old were chosed as the clinical case according to the PADAM diagnosis standard, and were divided into 3 groups according to the block stochastic principle, the observation group (acupuncture combined with BUSEHN capsule), group 1, group 2 (takes orally BUSEHN capsule group), each group had 20 cases, and each group of patients was measured by the method of radio-immunity for the serum level of testosterone, luteinizing hormoha, follicle-stimulating hormone before and after the treatment. By the contrast of the grade of PADAM symptom among three groups before and after treatment ,we can reveal the whole funcamental characteristic of acupuncture combined with herb ,and discuss a new way to enhance the curative effect of acupuncture ,and summarize the endocrinous adjustment of acupuncture for the PADAM's patients and the orchidic improvement by the treatment of acupuncture.

在前期预实验的基础上，选择符合诊断标准的PADAM诊断标准的45-65岁男性患者临床病例60例，按照区组随机原则分为3组，观察组、对照组1、对照组2，每组各20例，采用放射免疫法分别测定各组患者治疗前后血清中血清睾酮、黄体生成素、卵泡刺激素的血清浓度的水平；通过三组及三组间治疗前后PADAM症状评分指数的对比研究，揭示针药结合的整体调节作用特色，探讨提高针灸疗效的新途径；总结出针刺对中老年雄激素缺乏综合征患者的内分泌的调节及针刺对睾丸的改善程度，探讨针药结合治疗本病的机制及针灸增效剂的应用前景，为今后进一步研究打下坚实基础。

Asthma Interleukin-17 Interleukin-10 Enzym e-linked immunosorbent assay

哮喘；白细胞介素17；白细胞介素10；酶联免疫吸附测定

The indexed of blood rheology、erythrocytic immunity、nail cockle microcirculation were examined before and after therapy in two groups.

把口腔扁平苔藓病人随机分为两组，分别在治疗前后测定血液流变学、红细胞免疫、甲皱微循环等指标。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷