免疫测定

It has been reported that F185K and C280S mutations of HIV1 integrase would improve the enzyme solubility, and the catalytical activity of the enzyme was the same as that of the wildtype enzyme in vitro.

coli中进行整合酶基因表达，SDSPAGE鉴定表达产物，亲和层析纯化蛋白，酶联免疫吸附实验方法测定整合酶的生物学活性。

Methods:30 children suffering from viral en- cephalitis were treated with UBlO,once every three days for three times.The control group was 20 healthy children,At pre-and post-treatment,the value of CD_3,CD_4,CD_8,CD_4/ CD_8.BcelI,NKcell,IL-R,IgG,IgA and IgM was determined and compared in both groups.

采用紫外线照射充氧自血回输治疗病毒性脑炎患儿30例，每隔3日照射1次，3次为1疗程，疗程前后测定 T 细胞亚群CD_3、CD_4、CD_8、CD_4/CD_8、B 细胞、NK 细胞、白细胞介素2受体(IL-2R)、免疫球蛋白 IgG、IgA、IgM 的变化。

Objectie Measuring the cell immune function after pre-operatie transfusion,to proide scientific eidence for the cerebroma patients who receied transfusion.

目的通过测定脑肿瘤患者围手术期输血对细胞免疫功能的影响，为临床脑肿瘤患者谨慎输血提供科学依据。

Molecular weights of the components were tested through SDS????PAGE. Then the reaction rates of IgE and IgG in asthmatic patients serum to chenopodium album pollen were counted by immunoblotting.

PAGE测定变应原各组分的分子质量；应用免疫印迹法检测各组分蛋白质与藜草花粉过敏性哮喘患者血清中sIgG和sIgE的反应率。

Methods Serum contents of hyaluronic,type IV collagen,cholalic acid,tumor necrosis factor,transforming growth Factor-α,interleukin-1(IL-1),interleukin-6(IL-6)and interleukin-8(IL-8)were detected by radioimmunoassay and were analyzed.

方法应用放射免疫法对患者血清透明质酸、IV型胶原、胆酸、肿瘤坏死因子、转化生长因子-α、白细胞介素-1(IL-1)、白细胞介素-6(IL-6)和白细胞介素-8(IL-8)进行测定和分析。

Methods Utilized competitive radio-immunoassay surveyed CEA simultaneously both cholecystic bile and serum of 75 cases.

应用竞争放射免疫分析法共测定胆囊胆汁及同期血清CEA值 75例。

A polyclonal antibody against vitellin was prepared in male rabbits and the specific antibody against vitellin was made through absorbtion by male worm homogenate. After purification, HRP was used to mark antibody, then ELISA was set up to detect vitellin content in the Chrysopa septempunctata fat-body, hemolymph and ovary.

提取了大草蛉的卵黄蛋白，免疫家兔，并将所得抗血清经雄虫匀浆液吸收，获得抗卵黄蛋白的特异抗体，经纯化后，用辣根过氧化物酶标记抗体，建立了ELISA法测定大草蛉脂肪体、血淋巴和卵巢中卵黄蛋白含量的方法。

To study the function of chymase,the protease inhibitors including Lisinopril,Aprotinin and EDTA,and radioimmunuoassy were used to measure the activities of angiotensin converting enzyme and chymase in the heart tissues of transgenic mice harboring the human heart chymase gene and the controls.

为研究糜酶的功能，本文利用蛋白酶抑制剂Lisinopril、Aprotinin及EDTA结合放射免疫分析的方法同时测定了糜酶转基因小鼠心脏组织中血管紧张素转换酶（angiotensin converting enzyme，ACE）及糜酶样活性。

Methods: 50 normal Wistar male rats were randomly divided into five geoups: the sham operated group, injury model group, shenjindan capsule treat group, high dose and low dose group (duzhongyaotong pill treat, D and E)(n=10each). Autogenous nucleus pulposus was removed from the coccygeal vertebral and placed into the back of left L5~6 nerve root, The modle of the non-compressive nucleus pulposus protrusion was created by transplanting autogenous nucleus pulposus in the back of left L5~6 nerve roots. At tow weeks, the SP in dorsal root ganglion was determined by immunohistochemistry.

取健康Wistar大鼠50只，随机分为假手术组、模型组、对照组、高剂量组（D组、腰腿理痛散高剂量组）、低剂量组（E组、腰腿理痛散低剂量组），每组10只，将大鼠自身的尾椎髓核取出移植于左侧硬膜外腔L5、L6神经根背侧，造成非压迫性大鼠自体髓核移植硬膜外模型，通过免疫组化的方法测定大鼠2周时DRG中CGRP的变化。

Methods: Integrated optical density was measured by ABC immuno-histochemical and in situ hybridization technique with computerized image processing. The rat′s brain was coronally sectioned after combination of EA and MEL.

采用ABC免疫组织化学和原位杂交组织化学技术，结合计算机图像处理，测定其积分光密度值。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷