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免疫测定

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METHODS The level of immuno-protein, addiment (C3 and C4) and protein in colostrum for 60 mothers are tested by single dimensionaldoffusion and binret test.

应用单向扩散法和双缩脲法分别测定30例早产产妇初乳和30例足月产产妇初乳中免疫球蛋白sIgA、IgA、IgG、IgM、补体C3、C4和蛋白含量。

Cell cycle and surface antigen were detected with flow cytometry. Adipogenic differentiation and osteogenic differentiationwere tested by immunohistochemistry.

流式细胞仪测定细胞周期及细胞表型,免疫组织化学染色检测其体外诱导成脂肪和成骨分化的能力。

Methods: Thyroid and adrenocortical functions were assayed by radioimmunoassay in 26 leukemia patients before and after BMT.

用放射免疫法测定了26例白血病患者用MAC方案预处理骨髓移植前、后的甲状腺及肾上腺皮质功能。

The alveolitis and pulmonary fibrosis were examined by HE staining. The serum HYP was examined .The expression of CTGF was tested by immunohistochemical assay.

HE染色检测肺组织肺泡炎及肺纤维化程度;测定血清羟脯氨酸的含量;免疫组化染色检测CTGF的表达。

Methods: 60 SD rats were divided into 4 groups randomly, the nomal group, the model group, QZHJ group, and the hydrocortisone group. Rats were sacrificed in bdtch on the 3rd, 7th and 21st day after treatment, then the concentration of interleukin-8(IL-8) in bronchoalveolar lavage fluid and the content of transforming growth factor-β1 (TGF-β1) of lung tissue were deected, while the severity of alveolitis and fibrosis were measured.

将60只健康SD大鼠随机分为正常组、模型组、芪术合剂组和强的松组,经气管滴入博莱霉素复制肺纤维化大鼠模型,并分组予以治疗,于治疗后第3、7、21日分批处死大鼠,每组每次6只;用免疫组化法观察肺组织转化生长因子-β1的表达,并测定支气管肺泡灌洗液中中性粒细胞和白介素-8的水平。

Methods: Both IFN-γ and ADA of 80 specimens of pleural effusions (40 cases of tuberculosis pleural effusions and 40 cases of malignant pleural effusions) were analyzed by enzyme linked immunosorbet assay and ammoniacal reagent method.

用酶联免疫吸附分析法和氨试剂法检测80例胸腔积液(40例结核性积液和40例癌性积液)中IFN-γ水平和腺苷脱氨酶活性,并将IFN-γ检测结果与ADA测定结果进行对比。

At the same time seventeen normal ampullary oviducts during mid-secretory phase were included as control group. The routine two-step immunohistochemical technique was performed.

采用电化学发光法检测术前血β-HCG及免疫组织化学二步法测定各组标本中VEGF的表达,根据阳性率和表达强度进行组织化学评分和相关性分析。

The method uses radiative immunity analysis, determine 43 senile domentia and 33 hemal sexes are gawkish (the β in VD) patient and plasma of 58 healthy old people - the content; analysis of amylaceous appearance albumen and β of observation AD patient - the variable relation of the content of amylaceous appearance albumen and GDS process is reached and treat from beginning to end.

方法应用放射免疫分析,测定43例老年性痴呆和33例血管性痴呆患者及58例健康老年人血浆中的β-淀粉样蛋白的含量;分析和观察AD患者β-淀粉样蛋白的含量和GDS进程的变量关系及和治疗前后β-淀粉样蛋白含量的变化。

METHODS: The pregnant mice were administered by oral gavage with MXC at different doses of 0, 20, 100 and 200 mg/kg during day 12-17 of pregnancy. Meanwhile, other pregnant mice as a control group were gavaged sesame oil solvent in the same manner. The changes in body mass and number of corpora lutea of the pregnant mice were observed; and the body mass and anogenital distance of their F1 foetuses were recorded, too. The concentrations of estradiol (E2 ) and progesterone in sera of the pregnant mice were determined using radioimmunoassay.

将交配成功的雌鼠于孕12~17 d每日给予不同剂量的MXC(0,20,100,200 mg/kg)灌胃,并设芝麻油溶剂为对照组,观察各组孕鼠体质量和黄体等计数测定,同时观察各组F1子代仔鼠的体质量和肛门生殖器距离等,采用放射免疫法检测孕鼠血清雌二醇(E2)和孕酮含量。

The patient avoided eating food rich of protein for three days and fasting for 12 hours before the blood samples were obtained at 7:00-8:00 in the morning, and had laid on bed for 15 minutes at least, 2ml of the blood samples were taken from median antebrachial vein, put into the heparinized tubles, then centrifugating by 1500/minute for 10 minutes,obtained the plasma from the blood, the plasma sample were kept in -72℃ refrigerator, ELISA method was used to determine the homcysteine level.

患者在标本采集前3天避免食用高蛋白饮食,空腹12小时以上,清晨7:00-8:00采血,采血时平卧位,休息15分钟以上,采集肘正中静脉血2ml,置于肝素抗凝管,以1500转/分离心10分钟后,分离血浆,用酶联免疫吸附法测定各管的HCY含量。

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