免疫测定

Indirect immunofluorescence assay and ELISA were used to detect CD4+/CD8+ lymphocyte subpopulation and IgG antibody.

分别用间接免疫荧光法和ELISA法测定T淋巴细胞CD4＋／CD8＋亚群及特异性IgG抗体。

METHODS: A series of N-octyl-N'-succinyl chitosan labeled with fluorescein, were incubated with HepG2 liver cancer cells, K562 leukemia cells, A549 human lung cancer cells, and BGC cancer cells respectively and followed by using flow cytometry and enzyme-linked immunosorbent assay to assess the affinity and inhibition of N-octyl-N'-succinyl chitosan for the four kinds of tumor cells already mentioned.

用异硫氰酸荧光素对N-正辛基－N'-琥珀酰基壳聚糖进行标记，再分别与人肝癌细胞(HepG2)、人白血病细胞(K562)、人非小细胞肺癌细胞(A549)及人胃癌细胞共培养，通过流式细胞仪及酶联免疫检测仪测定N-正辛基－N'-琥珀酰基壳聚糖对肿瘤细胞的亲和性及抑制力。

An indirect competitive enzyme-linked immunoassay was developed using polyclonal antibody to measure the residues of sulfaquinoxaline in food.

利用抗磺胺喹噁啉多抗建立酶联免疫分析方法，以测定SQ在动物源食品中的残留。

Methods:Using cell culture and immunohistochemical techniques,the peripheral blood specimens from 33 RA patients were designed to test the human T cell lymphotropic virus type Ⅰ related antigens,reverse transcriptase and spontaneous syncytium formation.

采用细胞培养、免疫组织化学技术等对33例RA患者的外周血样本进行人类嗜T淋巴细胞病毒Ⅰ型相关抗原、逆转录酶活性测定及观察自发性合胞体形成。

Methods The serum SIL-2R level in syphilitic patients of different stages and after therapy were detected using the ELISA technique.

采用酶联免疫吸附法测定 70例梅毒患者及其治疗后SIL - 2R水平，并与 2 0名健康体检者进行比较。

Objective To assess error and clinical significance of technetium (99Tcm) counts measured by radio-immunity counting-meter.

目的 评价使用γ放射免疫计数仪测定得90Tc(上标 m样本计数的误差及临床意义。

Methods To measure the levels of ET-1 in the tissue fluid from vitiliginous lesion and the normal skin by the method of radio immunity.

方法采用放射免疫方法测定27例白癜风患者在NB-UVB治疗前后的白斑部位和非白斑的皮肤组织液中ET-1含量。

Methods SYBR Green I real-time polymerase chain reaction was used to detect the expression of VEGF mRNA in articular cartilage from 20 OA patients and 10 controls with traumatism. VEGF protein was detected by enzyme-linked immunosorbent assay in articular cartilage.

应用SYBR Green Ⅰ实时定量聚合酶链反应检测20例OA和10例外伤患者关节软骨中VEGF mRNA表达，并用酶联免疫吸附试验测定关节软骨组织VEGF含量。

Methods:Serum concentration of sICAM-1 and CRP of 22 cases were measured by ELISA and turbidimetry,respectively.

分别以ELISA法和免疫速率散射比浊法，测定了22例肺癌患者血清sICAM-1及CRP水平。

Methods Monoclonal antibody turbidimetry and ELISA methods were used to detect the plasma Lp and CEA in 56 patients with large intestinal cancer and 87 healthy persons.

采用单克隆抗体浊度法和酶联免疫吸附试验法测定了 5 6例大肠癌患者和 87例健康者的血浆脂蛋白和 CEA的含量。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷