免疫测定

Concentrations of DA and 5-HT in different regions were measured by high performance liquid chromatography; the expression of SERTmRNA was detected by in situ hybridization; the degeneration of neuron and terminal was demonstrated by Bielschowsky and Glee Marsland silver staining; and the protein expression of GFAP was detected by immunohistochemistry.

2采用高效液相色谱法测定不同脑区神经递质DA和5-HT的含量；采用原位杂交方法检测SERTmRNA；Bielschowsky及Glee Marsland氏改良银浸染法显示神经元胞体、树突和轴突；免疫组织化学方法检测GFAP的表达。

E and Sirus red in supersaturated picric acid solution;the amount of HA in serum was measured with the method of radiative immunity;the quantitative expression of fibrosis factor—TGF、PDGF was measured with the method of immunity histochemistry.Results:Myocardial collagen in model group increased obviously compared with normal group from the results of electron microscope、H.E、Sirus red in supersaturated picric acid solution.

腹腔注射柯萨奇B3病毒建立病毒性心肌炎慢性期心肌纤维化小鼠模型，随机分为模型对照组、通心络低剂量组、中剂量组、高剂量组，并设正常对照组，用药四周后处死动物通过透射电镜观察心肌细胞超微结构；HE染色、苦味酸天狼星红染色观察心肌胶原数量；放免法测定血清透明质酸的含量；免疫组化染色观察致纤维化细胞因子—转化生长因子和血小板衍生生长因子的定量表达。

Methods:Establish the model of myocardial fibrosis in chronic stage of viral myocarditis by injecting CVB3 into Balb/c rats,divide these rats into model group、Tong xin luo low dose group、Tong xin luo medium dose group、Tong xin luo high dose group and set up normal group,after four weeks of giving medicine execute the animals and observe the ultrastructure of myocardial cell with tramission electron microscope,the quantity of myocardial collagen was observed by staining with H.E and Sirus red in supersaturated picric acid solution;the amount of HA in serum was measured with the method of radiative immunity;the quantitative expression of fibrosis factor—TGF、PDGF was measured with the method of immunity histochemistry.

腹腔注射柯萨奇B3病毒建立病毒性心肌炎慢性期心肌纤维化小鼠模型，随机分为模型对照组、通心络低剂量组、中剂量组、高剂量组，并设正常对照组，用药四周后处死动物通过透射电镜观察心肌细胞超微结构；HE染色、苦味酸天狼星红染色观察心肌胶原数量；放免法测定血清透明质酸的含量；免疫组化染色观察致纤维化细胞因子—转化生长因子和血小板衍生生长因子的定量表达。

This assay was applied to determination of HRP in the solution of hepatitis B surface antigen labeling HRP, with satisfactory results.

该法应用于酶联免疫乙肝试剂盒中辣根过氧化物酶活力的测定，结果比较满意。

There were inflammatory cells infiltrating in the myocardium sparely and no calcification in the pericardium in IVIG and IVIG+VitC groups. The size of spleens enlarged especially in IVIG and IVIG+VitC groups.

心脏分为三部分：一部分作病理切片和H-E染色，一部分作冰冻切片，在荧光显微镜下用直接免疫荧光法测定心肌组织中沉积的IgG水平，一部分作电镜检查。

The spindle density of collilongus and splenius of head was determined and the expressions of the NT3 and Trk C in dorsal root ganglions were observed immunohistologically.

经甲醛固定1年以内的尸体12具，按年龄分为婴儿组5具，青少年组3具，老年组4具，测定其颈深肌群中的颈长肌及头夹肌肌梭密度，应用免疫组化的方法观察NT3、Trk C在相应背根节神经元的表达。

Methods Expressions of HPV6, 11, 16, 18, 31, 33,42,51,52,56 and 58 were determined using SP staining of immunohistochemistry.

用免疫组化SP染色法测定组织中HPV6、11、16、18、31、33、42、51、52、56、58的表达。

Methods All cerebrospinal fluid cells (control group and meningeal carcinomatosis) were stained with CEA by immunofluorescence, scanogram and fluorescent intensity stereophotography were obtained and finally fluorescent values of CEA were individually measured by LSCM.

应用免疫荧光细胞化学法以CEA标记脑膜癌细胞及对照组脑脊液细胞，采用LSCM技术获得脑膜癌细胞扫描图像并定量测定CEA荧光强度值。

After spraying 3 kinds of growth regulators PBO,PP333 and GA_3 after full bloom,the concentration of which was 50 mg/L,the content of soluble sugar,titratable acid,soluble solid substance,vitamin C,and flesh hardness,fruit shape index,stone cell content were analyzed,and the content of IAA,GA_3,ZR and ABA in young fruit was determined by means of ELISA method to probe the dynamic change of the endogenous hormones during the fruit development.

在此基础上，于盛花期喷施浓度均为50 mg/L的生长调节剂PBO、PP333、GA_3，对各处理果实可溶性糖、可滴定酸、可溶性固形物含量及果肉硬度、果形指数和果实石细胞含量进行分析；采用酶联免疫吸附法测定了幼果生长发育过程中IAA、GA_3、ZR和ABA含量的动态变化。

Methods The MMP-3, OPN, and TIMP-1 expressions in LECs of anterior subcapsular cataract (31 cases), nuclear cataract (28 cases) and normal lens (12 cases) were detected by immunohistochemistry, respectively.

采用免疫组织化学方法测定MMP-3，OPN和TIMP-1在31例前囊下性白内障，28例核性白内障和12例正常晶状体上皮细胞中的表达。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷