免疫测定

The objective of this study was to identify how many different forms of GnRH in the black porgy brain by HPLC and to localize GnRH fiber and soma in the brain by immunohistochemistry. In addition, we also study the effects of 17- estradiol on the hypothalamic gonadotropin-releasing hormone soma number in the black porgy.

本实验希望藉由HPLC来测定黑鲷脑中GnRH的种类，并利用免疫染色的方法，了解GnRH神经元在黑鲷脑中的分布，并探讨雌二醇(17β-estradiol； E2)对切除性腺(gonadoectomy； GNX)的黑鲷脑中下视丘部位GnRH神经细胞本体个数之影响。

Methods Twenty five healthy volunteers were subjected to ETUS on the precordial region, and the parameter of ETUS was 0.8MHz, 1.2W/cm 2, the subjects were treated for 15 minutes daily for 7 days.

采用酶联免疫吸附法及发色底物法测定正常人经ETUS作用前、后，其血浆GMP 14 0、TXB2 、t PA及PAI 1含量的变化，并将经ETUS作用前、后的相应数据进行比较。

Using Gαq/11 polyclonal antibody as prole and immunoblot analysis, we measured Gαq/11 contents in left ventricle of rats. There was a significant decrease in Gαq/11 content of CA rats compared with values of control animals after 3 days of operation (P＜0.01). Gαq/11 contents were not modified in left ventricle after 10 and 30 days of stenosis.

以兔抗鼠Gαq/11多克隆抗体为探针，用免疫印迹方法测定了大鼠左心室组织中Gαq/11蛋白的相对含量，结果发现术后3天CA组大鼠左室中Gαq/11含量较SO组明显降低(P＜0.01)，术后10天和30天二组大鼠心肌中Gαq/11含量无差异(P＞0.05)。

Abstract］ Objective: To investigate the effect of polysacchaide of silkworm pupa on immunological function in mice.

目的：通过测定蚕蛹多糖对小鼠淋巴细胞增殖、巨噬细胞吞噬活力及溶血素生成的影响，研究蚕蛹多糖对小鼠免疫功能的影响。

Objective: To investigate the effect of polysacchaide of silkworm pupa on immunological function in mice.

目的：通过测定蚕蛹多糖对小鼠淋巴细胞增殖、巨噬细胞吞噬活力及溶血素生成的影响，研究蚕蛹多糖对小鼠免疫功能的影响。

Methods:The expression of CK20mRNA in portal venous blood from 30 colorectal cancer patients was detected by fluorescent quarto RTPCR,and the results of CD44v6 and PCNA in colorectal cancer tissue were determined by means of immunohistochemistry,and then compared with control groups through statistics analysis.

应用荧光定量RTPCR法检测50例大肠癌患者回流门静脉血中CK20mRNA，同时应用免疫组织化学方法测定大肠癌癌组织中CD44v6、PCNA的表达，并与对照组比较。

Tumor Necrosis Factor-alpha ;; Pharmacokinetics ;; Radiometry ;; Enzyme-Linked Immunosorbent Assay ;; Methods ;; Lymphotoxin-alpha

肿瘤坏死因子-α；；药代动力学；；放射测量术；；酶联免疫吸附测定；；方法；；淋巴毒素

Methods Twenty-six NZW rabbits were randomly divided into three groups: a normal control group (fed on normal commercial rabbit diet, n =6), a hyperlipidemic group fed on high-fat ma total cholesterol (TC concentration at the beginning and the 12-week end point of the experiment determined,and all the experimental rabbits were sacrificed, and endothelial function test were performed on thoracic aortic rings prepared from the isolated aorta using the vascular endothelium-dependent relaxation parameter in response to acetylcholine The aortic atherosclerotic lesion expression of LOX-1 mRNA and protein were examined by RTPCR and Western blotting respectively.

方法新西兰大白兔随机分为正常对照组(普通饮食，6只)，高脂饮食组(高脂饮食，10只)及普罗布考组(高脂饮食+普罗布考200mg/kg·d，10只)。实验开始前及第12周分别耳缘静脉采血测定血清总胆固醇。第12周处死动物，取胸主动脉，制备离体胸主动脉环对乙酰胆碱的反应以检查内皮功能，RT-PCR与免疫印迹检测凝集素样氧化低密度脂蛋白受体-1(LOX-1)基因与蛋白质表达水平。

Radioimmunoassaywas utilized to measure levels of angiotensin Ⅰ, angiotensin Ⅱ and plasma rennin activity, respectively.

用放射免疫法测定血管紧张素Ⅰ、血管紧张素Ⅱ和血浆肾素活性。

To evaluate the methodology and clinical application of turbidimetric immunoassay for detection of retinol -binding protein in serum.

对视黄醇结合蛋白免疫透射比浊测定法进行方法学和临床应用评价。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷