免疫测定

To record the change of parathormone, complement(C3,C4),albumin,paltelet and the remission conditions of restless legs syndrome before and after the first dialysis ,and after two weeks and four weeks,to compare the indexs in each group.

记录首次透析前后及透析2周、4周后血甲状旁腺激素、补体（ C3、 C4）、清蛋白、血小板等的变化及不安腿综合征临床症状缓解情况，对各组指标进行比较。放射免疫法测定血清 PTH 水平。

In group A, glutamine was added enterally, and in the group B, glutamine was added parenterally.

测定3组的转铁蛋白、前白蛋白、氮平衡、免疫球蛋白、CD4／CD8、感染发生率等指标。

Methods The biopsy specimens in paries anterior vaginase were obtained from 88 subjects undergoing hysterectomia,tension-free vagina tape,Burch procedures,who were assigned into control,pelvic organ prolapse(POP 28 cases) and SUI groups30 c...

方法采用免疫组化方法，测定30例压力性尿失禁患者、28例盆底器官膨出患者阴道前壁组织中TGF-β1及ER阳性率，并选择同期30例非卵巢功能性肿瘤和宫颈病变患者作为对照。

The expression of Bcl22 proteins in the ischemic penumbra were examined by immunochemical technique.

免疫组织化学法测定脑组织缺血半影区Bcl22蛋白的表达。

Serum pepsinogenⅠ and pepsinogen Ⅱ levels were measured by radioimmunoassay in 190 healthy controls, 103 patients with gastric cancer before operation, 33 patients after partial gastrectomy, 18 patients after total gastrectomy, and 23 return visit patients after total gastrectomy.

运用放射免疫分析方法测定了190例正常人，103例胃癌手术前病人，33例胃部分切除病人，18例全胃切除病人，23例全胃切除后随访病人的血清PGⅠ、PGⅡ的含量，并进行统计学分析。

Methods Core b1od mononuclear cells were isolated by Ficoll, Cd34(superscript +) cells were purified from mononuclear cells by MACS and then cultured in a complete medium containing rhIL-4, GM-CSF, rhSCF, rhFlt-3 Ligand, TGF-β1 and TNF-α. After 14d of incubation, the morphology of the cells was observed, the phonotype was examined and the IL-12 level in the medium was measured.

Ficoll分离脐血单个核细胞，免疫微磁珠法分离纯化CD34细胞，以rhIL-4、GM-CSF、rhSCF、rhFlt-3 Ligand、TGF-β1，及TNF-α体外诱导培养14d，进行形态学观察、表型检测及上清液IL-12测定。

In order to overcome the shortcomings of acellular dermal matrix used as tissue engineering scaffold material, such as bad permeability, low degradation speed, and strong immunogenicity, a new type of collagen scaffold was prepared by applying various chemical, biological and physical comprehensive methods to deal with pigskin. The microstructure of the scaffold was observed under photomicroscope and scanning electron microscopy, and the in vitro degradation time, permeability, tensile-strength, porosity, shrinkage temperature were tested.

为了克服猪脱细胞真皮基质作为组织工程支架材料渗透性差、降解速度过慢、免疫原性较强等缺点，采用多种化学、生物与物理综合方法处理猪皮制备了一种新型天然胶原支架材料，通过光学显微镜、扫描电镜观察以及体外降解时间、透水汽性、拉伸强度、孔隙率、收缩温度等的测定，对其性能进行了研究。

Methods The subjects were divided into two groups: PIH group (n= 15) and normotensive control group (n=15). Peripheral lymphocytes of the subjucts were separated by Ficoll-Hypaque density centrifugation. The lymphocytes were cultured in RPMI-1640 medium with or without pokeweed mitogen for ten days.Then the supernatant was collected and the IgG and IgM were measured by enzyme linked immunosorbent assay.

采用密度梯度离心法分离妊高征组(15例)和正常妊娠组(15例)的外周血B淋巴细胞，并在有或无美洲商陆有丝分裂原存在的情况下培养10天后，收集上清液，采用酶联免疫吸附试验测定每1×106个B淋巴细胞所分泌的IgG和IgM的量。

PAP-C23, amplified from mature pokeweed antiviral protein gene by PCR, was cloned into the expression vector PET101, and then the recombinant plasmid was transformed into Escherichia coli BL21(DE3). The recombinant mutant PAP was expressed high-levelly induced with IPTG.

用KCL溶液染SDS-PAGE胶，切下表达的目的蛋白，冰浴研磨后用磷酸缓冲液溶解，再加入等体积的福氏不完全佐剂，乳化完全后，免疫家兔，制备抗PAP蛋白的多克隆抗体，间接ELISA法测定所制备的抗血清的效价为1：1000。

Recently, it is proved that SP is also involved in pain modulation, acupuncture analgesia and immunoin- hibition. However, the role of CGRP in them is unclear Therefore, the dy- namic changes of CGRP-, SP-like immunoreactivity and expression ofα- CGRP,β-CGRP mRNAs in dorsal root ganglion and spinal cord in the arthritic rats were observed by using immunohistochemistry and in situ hybridization techniques combined with behavioral tests, respectively. Subsequently, the effects of this peoptide on the develoment of polyarthritis were investigated by intrthecal injections of CGRP and its antagonist in the rats. In addtion, GAL is a novel neuropeptide which coexists with CGRP and SP, but it may play a different role in the primary sensory system. Thus, we also observed the changes of GAL and GAL mRNA in DRG neurons and the spinal cord. The results were as follows

为此本文拟在大鼠佐剂性关节炎模型上，结合行为学测定、用免疫组织化学、原位核酸分子杂交技术观察脊髓背角及DRG中CGRP、SP、α-CGRP mRNA、β-CGRPmRNA的动态变化及电针的影响，此外，用鞘内和外周注射CGRP的工具药探讨了CGRP在佐剂性关节炎发生发展中的可能作用；甘丙肽（Galanin，GAL）也是新近发现的一个神经肽，并与CGRP和SP共存于DRG及初级传入末梢中，在神经损伤模型中脊髓CGRP、SP减少，GAL则明显增加，显示GAL与CGRP和SP的作用可能不同，因此在佐剂性关节炎模型上也同时观察了GAL和GAL mRNA的变化，实验结果如下

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。