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Methods BALB/c mice were inoculated muscularly with HIV-2 gag recombinant DNA vaccine, or recombinant fowlpox virus, or both.

大量制备并纯化HIV-2 gag重组DNA疫苗和重组鸡痘病毒,以肌肉注射的方式免疫BALB/c小鼠,ELISA法检测小鼠血清HIV-2抗体,流式细胞仪测定CD4^+、CD8^+T淋巴细胞亚类数量,乳酸脱氢酶释放法检测脾CTL对HIV-2靶细胞的杀伤活性。

Methods The level of CEA, Pro-GRP, CYFRA21-1 and β 2-MG in serum were determined by using electric chemistry irradiance, enzyme-linked immunosorbent assay and immunity nephelometer respectively in 115 patients with lung cancer,41patients with benign lun...

分别采用电化学发光法、ELISA法和免疫浊度法测定115例肺癌、41例良性肺病患者和50例健康献血员的血清CEA、Pro-GRP、CYFRA21-1及β2-MG水平并作对比分析。

Methods Plasma D-dimer levels of 360 healthy donators were determined by automatic coagulation analyzing immune nephelometry and conducted comparing observation.

采用全自动血凝分析透视光免疫浊度法对不同年龄段的360名健康献血者,进行体内血浆D-二聚体的含量测定,并进行比对观察。

METHODS: The label index of Ki-67 was studied by immunohistochemical method in the tissue of superficial lobe parotidectomy which included the primary and secondary operation cases, nonrecurrent group, and group of low-grade malignant parotid gland tumors.

运用免疫组织化学方法,测定并比较细胞增殖活性相关抗原Ki-67在腮腺多形性腺瘤腮腺浅叶切除术后未复发病例、复发前病例、复发后病例及腮腺低度恶性肿瘤病例术后石蜡标本中的阳性表达率。

The different expression of Bax between group C and group B was nonsignificant.

应用免疫组化SP法结合病理图像分析仪测定肝组织中Bcl-2、Bax的表达情况;。

Methods:Serum IGF 1 levels were measured by specific radioimmunoassay in 46 patients with essential hypertension before and after three months of treatment with fosinopril as well as in 18 normotensive controls.

用特异性的放射免疫分析法测定46例原发性高血压患者福辛普利治疗前和治疗3 个月后及18例正常对照组的血清IGF-1 水平。

INTERVENTIONS:The content of serum leptin and insulin in 41 hypertensive and 21 normotensive elderly men was determined by the method of radioimmunoassay.

用放射免疫法测定 41例高血压和 21例正常血压的老年男性患者血清瘦素和胰岛素的含量。

All rats were killed 8 days after operation and the expression of transforming growth factor-13 was detected by immunohistochemical method.

术后第8天分别处死各组大鼠,用免疫组织化学方法测定转化生长因子日的表达情况。

Methods: The effects of Opuntia on proliferation of fibroblast were measured with MTT colorimetric assay, and PCNA productions were examined with immunocytechemistry.

以小鼠成纤维细胞株(NIH3T3)为研究对象,采用四氮唑盐比色法测定仙人掌对成纤维细胞增生的影响,采用免疫细胞化学染色法检测成纤维细胞的增殖细胞核抗原的表达。

METHODS: EO contents of plasma and tissues in 1k1c HR were detected by ELISA. The relationships between plasma and tissues ouabain and blood pressure were analyzed.

采用酶联免疫吸附试验测定"1k1c"高血压肿瘤大鼠血清及多种组织内EO含量的改变,并对体内EO含量与大鼠血压进行相关性分析。

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