免疫测定

Indirect immunofluorescence was used to analyze the activity and specificity of the mAb.

用间接免疫荧光法对mAb的抗体活性及特异性进行测定。

MATERIALS AND METHODS: The protein expressions of MMP-7 and MMP-9 were evaluated by immunohistochemistry in 18 normal villi. 49 hydatidiform moles, in which 8 were malignantly transformed moles.

材料与方法：采用免疫组织化学PV-6000法测定49例葡萄胎（其中8例恶变，列为葡萄胎恶变组）和18例正常绒毛组织中的MMP-7和MMP-9蛋白表达量。

Methods 43 patients with IBS and 20 healthy subjects were comparatively investigated. Anorectal pressure was measured with anorectal catheta of solid state manometric transducers.Gastrointestinal hormones in plasma including gastrin,glucagen,were measured with radioimmunossay.

采用五通道环状固态压力传感器测压导管测定43例IBS患者直肠肛管的压力，以及采用放射免疫分析法检测血浆胃泌素、胃动素、胰高糖素的水平，并与20例正常人作对照。

Methods The activity of PTK, PKCα,βI ,βII ,γ,ε,ξ and the active station of NF-κB in large bowel carcinoma tissues was detected by using membraneous conjugated method, laser scanning confocal microscopy and immuriohistochemistry respectively in the animal model of nude mouse transplantation tumors of large bowel carcinoma.

以大肠癌裸鼠移植瘤为动物模型，用膜结合法、激光共聚焦显微镜和免疫组织化学分别测定了大肠癌组织蛋白酪氨酸激酶、蛋白激酶Cα、βI、βⅡ、γ、ε和ξ的活性以及核因子-κB的活化状况。

Aflatoxins were enhanced by mercury chloride solvent online and detected by fluorescence detector.

采用免疫亲和方法进行样品前处理，用甲醇－乙腈－水三元流动相体系分离黄曲霉毒素，氯化汞溶液在线衍生，荧光检测器检测，建立了新型的高效液相色谱柱后衍生测定黄曲霉毒素(B1、B2、G1、G2、M1)的方法。

Methods: Twenty-four Kkay mice were divided into three groups(n=8):diabetes group,methionine-diet group,folic acid and vitamin B12 treatment group. C57Bl/6 mice were used as controls(C57). Four months later,the mice were sacrificed and their serum levels of Hcy,folic acid and vitamin B12 were measured by fluorescence polarization immunoassay. Pathological changes in kidneys were examined with the periodic acid schiff method with periodic acid-silver methenamine staining under electron microscope. Expression of MMP-9 protein or mRNA was detected by RT-PCR with immunohistochemical staining. Results: Different morphological changes of DN were found in Kkay mice.

Kkay糖尿病鼠随机分3组：糖尿病组、高蛋氨酸组和叶酸、维生素B12组，C57BL/6鼠为对照(C57)，以相应饲料喂养4个月后取材，测定血糖、血同型半胱氨酸、叶酸和维生素B12，以PAS、六氨银染色法光镜下及超微电镜观察各组DN病变，比较各组肾小球硬化指数；以免疫组化和半定量图像分析肾小球MMP-9蛋白表达，并以RT-PCR检测肾脏MMP-9 mRNA水平。

An enzyme immunoassay method for the determination of choramphenicol residues in infant formula powders is presented.

目的〕建立测定婴幼儿配方奶粉中氯霉素残留的酶联免疫分析法。

The expression of proliferating cell nuclear antigen in each group was detected by immuno-histochemistry method. The cell cycle distribution was measured by flow cyto- metic analysis.

MTT法及免疫组织化学染色增生细胞核抗原法检测细胞生长活性，流式细胞仪测定细胞周期时相变化。

In vitro myocardial cell culture, the distribution and the content change of the myocardial cytoskeleton were observed by immunocytochemistry technique and FCM, and the viscoelastic properties of myocardial cells were measured by micropipette aspiration experiment.

离体心肌细胞培养，采用免疫组化及流式细胞术观察心肌细胞骨架蛋白分布和含量变化，并通过微管吸吮实验测定心肌细胞粘弹力学特性变化。

Objective To diagnose minor myocardial damage in unstable angina and evaluate the prgnostic value of MMD,according to the results of serum cardiac troponin Ⅰ detected by immunoradiometric assay.And to discuss the selection of the most serious subgroup of unstable angina.

目的 根据血清cTnⅠ免疫放射测定的结果，判断不稳定性心绞痛(Unstable Angina，UA)患者的微小心肌损害(Minor myocardial damage，MMD)的情况，及其对预后的影响，进一步探讨UA患者高危亚组的筛选。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷