免疫测定

METHODS: AT Ⅰ,Ⅱ and ALD levels of all patients under laparoscopy were observed by RIA respectively at 10 min before insufflation, 10 min, 30 min and 60 min after insufflation and 10 min after desufflation.

在气腹前10 min，气腹后10，30和60 min，解除气腹后10 min采静脉血，用放射免疫方法测定血浆中ATⅠ，Ⅱ和ALD含量。

Strains M110 and M113 isolated from Aedes albopictus and Armigeres durhami, respectively, which were fatal to suckling mice at 4 days after intracerebrally inoculation and had cytopathologic effects on BHK21 cells, showed a positive reaction to dengue antibody and could be amplified Ly using flavivirus primers and dengue type-4 NS1 gene primers and dengue type-4 NS2a gene primers.

结果 从白纹伊蚊和达勒姆阿蚊中各分离到一株病毒，分别命名为M110和M113，这两株病毒对BHK21细胞能产生明显的细胞病变，脑内接种乳鼠4d左右导致乳鼠死亡。该病毒经血凝、血凝抑制和间接免疫荧光试验提示为黄病毒。分别用黄病毒属特异引物、登革4型病毒NS1和NS2a基因片段特异引物进行RT-PCR扩增、序列测定，证实为登革4型病毒。

IPTG induces the expression protein of GST-pUL49j and the induced product was purified electroeluting.The PAGE mortar contained GST-pUL49j was used as antigen, injected intradermally to immunize male New Zealand white rabbit to get polyclonal antiserum.

以含蛋白GST-pUL49j的PAGE胶泥做为抗原，皮内多点免疫注射雄性新西兰兔制备多克隆抗血清，ELISA测定多克隆抗血清效价，Western blot检测多克隆抗血清的特异性。

Appraised the clinical effect of the flavone of rhizome drynania on Senile Osteoporosis, according to the dynamic observation on integrative scores of lumbodorsal pain and lumbodynia,"VAS section grading method" on lumbodynia,"Barthel index rating method" on ADL, detected BMD on anterior-posterior position lumbar2~4 vertebra body and proximate of femur including neck/ward/troch, and determined the index of bone transition including BGP/PINP/ICTP through the radioimmunity method before and after treated half a year, then contrast intragroup and selfgroup. Depending on the standard of curative effect, contrast the difference of curative effect between two groups.

采用随机对照的方法，分为治疗组36例，对照组34例，依据对腰背痛及腰痛综合评分进行动态观察；依据"疼痛VAS线段分级法"对腰背痛（静止痛、翻身痛、久坐痛、起身痛、弯腰痛、步行痛、睡眠痛、叩击痛、压痛）进行量化动态观察；依据"Barthel指数评定法"对老年性骨质疏松症日常生活活动能力进行观察；采用双能X线骨密度仪对腰椎(L2-L4)、股骨近端各部位：股骨颈、股骨三角区、股骨粗隆进行骨密度定量检测；应用放射免疫法对骨转换指标进行测定，骨形成指标：骨钙素、Ⅰ型前胶原氨基端前肽；骨吸收指标：Ⅰ型胶原交联羧基末端肽等，评价骨碎补总黄酮治疗老年性骨质疏松症的临床效果。

MTT colorimetric assay was used to measure the proliferation of VSMCs. The expression of intranuclear proto-oncogenes c-myc and proliferating cell nuclear antigen were observed by immunocytochemical stain and imageanalysis technique.

采用定磷法分别测定各组细胞CaM和CaN活性，MTT法检测细胞增殖活度的变化，免疫组化定量技术观察细胞内c-myc、PCNA的表达水平。

The mice were divided into three groups: a cardiac isograft of C57BL/6 to C57BL/6 mice; b cardiac allograft of Balb/c to C57BL/6 mice; c cardiac allograft of Balb/c to C57BL/6 mice with mfgl2 polyclonal antibody treatment.

采集移植心组织标本作病理学检查，以免疫组织化学方法测定mfgl2在移植心脏组织细胞上的表达，并对mgfl2的表达进行半定量分析。

METHODS: The models of abdominal aorta transplantation were made with micro-surgery in rats. The recipients were divided into three groups: allograft control group, atorvastatin-treated group and isograft control group. Vascular intimal thickness in all of the groups was observed by histological examination. The expression of proliferation cell nuclear antigenl and α-smooth muscle actin were determined by immunohistochemistry. The content of nitric oxide was measured by nitrate reductase chromatometry.

建立大鼠腹主动脉移植慢性排斥反应模型，分为3组：异系移植对照组、异系移植治疗组和同系移植组，60d后对移植动脉行病理组织学检测，观察移植动脉内膜增生程度；并行免疫组织化学染色，检测移植动脉增殖细胞核抗原和平滑肌肌动蛋白的表达情况，行硝酸还原酶比色法测定移植动脉组织NO的含量。

Objective:Analysis of screening results of three neonatal diseases to further clarify the incidence of phenyl ketonuria,congenital hypothyroidism and glucose-6-phosphate dehydrogenase(G6PD) deficiency in Hainan Province.

目的：对海南省新生儿疾病筛查情况进行分析，了解海南省新生儿苯丙酮尿症、先天性甲状腺功能低下症、葡萄糖6-磷酸脱氢酶缺乏症(G-6-PDD)的发病率。方法：对出生72h后的7412名新生儿采集足跟血制作血滤纸干标本，PKU筛查采用荧光定量法检测滤纸干血斑中Phe含量；CH筛查采用时间分辨荧光免疫法检测滤干血斑中TSH含量；G6PD缺乏的筛查采用荧光斑点定性试验测定G6PD活性。

And compared with 55 normal controls from their healthy kinsfolk matched in sex and age.

血清LN和PcⅢ含量的测定采用放射免疫法。

Methods In vitro, human gastric cancer cell line SGC-7901 in log phase was intervened by As_2O_3 (final concentrations in order:1.0,5.0,10.0μmol/L) and 5-Fu (final concentration:50mg/L) respectively alone or in combination, and incubated for 24h、 48h、 72h. Blank group is set up. The cell apoptosis and cell cycle changes were observed by flow cytometry, and the expression of apoptosis-related gene Bcl-2 was analyzed by immunocytochemical method and absolute gray scales quantitative method.

将对数生长期的人胃癌细胞株SGC-7901(终浓度为2×10~5)经低、中、高三种浓度的As_2O(终浓度分别为1.0，5.0，10.0μmol/L)和5-Fu(终浓度50mg/L)单独及联合处理，设空白对照组，分别孵育24h、48h、72h后，收取标本采用流式细胞术测定细胞凋亡率及细胞周期变化；免疫组化绝对灰度定量分析检测凋亡抑制基因Bcl-2的表达。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷