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免疫测定

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Methods: Expression of Ki-67 was determined in 59 cases of Barrett's esophagus, 5 severe reflux esophagitis, 5 esophageal a...

应用免疫组化SP法测定59例Barrett食管、5例重度反流性食管炎、5例食管腺癌和10例正常食管黏膜组织中Ki-67的表达。

Methods anti-tumor activity of Phellinus linteus was estimated by the longevity of EAC mice,the immune effect of tumorous mice was tested by the experiment of carbon particle expurgation and the IL-2 concentration in serum of tumorous mice was determined by ELISA.

以小鼠艾氏腹水癌的生命延长率评价桑黄的抗肿瘤作用,以炭末廓清实验观察荷瘤小鼠非特异性免疫功能,以ELISA法测定荷瘤小鼠血清IL-2浓度。

Methods The anti-tumor activity ofPhellinus linteus was estimated by the longevity of EAC mice,theimmune effect of tumorous mice was tested by the experiment ofcarbon particle expurgation and the IL-2 concentration in serum oftumorous mice was determined by ELISA.

以小鼠艾氏腹水癌的生命延长率评价桑黄的抗肿瘤作用,以炭末廓清实验观察荷瘤小鼠非特异性免疫功能,以ELISA法测定荷瘤小鼠血清IL-2浓度。

Immunohistochemical technique was used to examine the expression of FN in villus and deciduas of these three groups.

采用免疫组化法测定绒毛和蜕膜组织中FN的表达。

Methods Immunohistochemical method with two specific antibodies(MMP-9,CD68) was used to detect the their special antigens in lung tissues of six cases who died of SARS and five normal cases as the controls.Gomori aldehyde fuchsine staining method was used to explore the change of elastic fiber.Motic advanced 4.0 image analysis system was used to analyze those images.

方法通过免疫组织化学技术对6例SARS死亡患者肺和5例肺外伤患者手术切除的正常肺组织中MMP-9和CD68+细胞进行检测,Gomori醛品红法显示弹性纤维,应用Motic Advanced 4.0图像分析系统进行定量分析,测定MMP-9的面密度、CD68+细胞的数量、弹性纤维的含量,并与正常对照组进行比较。

CD45/SSC gating procedure was a stable and reliable method to test the immunophenotype of MM.

CD45/SSC设门法是一种稳定可靠的MM免疫表型测定方法。

Methods Bioptic samples were taken from 8 cases of hypertrophic scar, 8 cases of superficial scar and 16 cases of controlled normal self-skin.MC-1R immunohistochemistry staining and its grey scale were examined in each sample.

利用兔抗人MC-1R抗体进行免疫组织化学染色,并测定8例增生性瘢痕,8例表浅性瘢痕和16例同体的正常皮肤标本切片染色后的平均灰度值,分别检测MC-1R在上述不同组织中表达的差异。

MethodsBioptic samples were taken from 8 cases of hypertrophic scar, 8 cases of superficial scar and 16 cases of controlled normal self-skin.MC-1R immunohistochemistry staining and its grey scale were examined in each sample.

利用兔抗人MC-1R抗体进行免疫组织化学染色,并测定8例增生性瘢痕,8例表浅性瘢痕和16例同体的正常皮肤标本切片染色后的平均灰度值,分别检测MC-1R在上述不同组织中表达的差异。

Synthesis, purification and the ECL character of luminol-digoxinA new type of homogeneous ECL immunoassay is proposed by labeling luminol to hapten e.g.

建立 了测定鲁米诺和二茂铁的电化学发光分析法,对利用该体系建立均相电化学发先免疫分析方法有一定的促进作用。

Methods:YCP was coupled to bovine serum albumin to construct three neoglycoprotein s which are different in their degrees of substitution(DSs;≈3,≈6,or ≈10 mol of BSA carrier/mol of YCP hapten,respectively),and their immunogenicities were evaluated in mice following subcutaneous immunization,respectively.

将YCP与牛血清白蛋白共价偶联,获得取代度分别约为3、6和10的3种拟糖蛋白,经皮下分别接种ICR小鼠,测定免疫血清中多糖YCP的特异性抗体IgM和总IgG水平,以及半抗原和载体蛋白的特异性IgG亚型。

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