免疫测定

NF-κB expression was detected by immunofluorescence cytochemistry, and the viability of the PC12 cells was demonstrated by MTT assay.

免疫荧光细胞化学法测定软骨藻酸对PC12细胞NF-κB蛋白的影响；MTT法检测PC12细胞的存活率。

Serum pancreatic polypeptide both at fasting state and after giving compound datura stramonium suppository were measured by RIA in 21 cases of bronchial asthma and 16 cases of chronic bronchitis during attack.

本文用双抗体放射免疫法，测定了21例支气管哮喘和16例慢性支气管炎发作期空腹及用复方洋金花栓剂后血清胰多肽水平。

Immunohistochemical technique was carried out to examine the distribution of collagen type IV and fibronectin in decidua of both groups.

采用免疫组织化学法测定早孕蜕膜组织IV型胶原(对照组15例，药物组20例)和纤维粘连蛋白(对照组25例，药物组30例)的分布状况。

METHODS: Total 60 male Wistar rats were randomly divided into 6 groups, i.e., normal control group , DMN model group , large dose treatment group , middle dose treatment group, small dose treatment group and Dongbao gantai treatment group. The liver fibrotic model was induced by i.p. dimethylnitrosamine for 4wk. The relative herbs were given after model establishment in each treatment group and the administration lasted for 6 weeks. Decollate to get the blood and The serum hepatofibrosis indexes, hyaluronic acid, collagen IV, pro-collagen type III and laminin were measuredwith radioimmunoassay. SOD and MDA were measured with biochemistry method. The liver pathological changes were observed in each group of rats.

将60只雄性Wistar大鼠随即机分正常对照组、模型对照组、实验大剂量组(20ml/kg/d)、实验中剂量组(10ml/kg/d)、实验小剂量组(5ml/kg/d)及东宝肝泰药物对照组；腹腔注射二甲基亚硝胺制备大鼠肝纤维化模型，连续灌胃6周，断头取血，生化检测血清SOD、MDA；放射免疫法测定血清HA、LN、PCIII及IV-C；取肝左叶做HE染色和Mallory胶原染色，观察肝病理组织学改变。

METHODS: A limb ischemia-reperfusion injury model in rats was established. The animals were randomly divided into three groups: control group, IR group and IPC group. The contents of diamide oxidase, nitric oxide, endothelin-1 (ET-1) and ratio of nitric oxide/endothelin-1 (NO/ET-1) in the plasma and the gut were measured. The leavels of myeloperoxidase, ratio of DNA chain, total nitric oxide synthase, inducible nitric oxide synthase and constitutive nitric oxide synthase in the gut were determined.

雄性Wistar大鼠18只，随机分为对照组，缺血再灌注组和缺血预适应组，每组6只，分别测定血浆和小肠组织二氨氧化酶、一氧化氮、内皮素-1(ET-1)、NO/ET-1比值的含量变化及小肠组织的髓过氧化物酶、DNA双链百分率(ratio of DNA chain %)、总一氧化氮合酶、诱导型一氧化氮合酶、结构型一氧化氮合酶的水平；免疫组化法检测小肠组织的诱导型一氧化氮合酶、内皮型一氧化氮合酶的表达。

Take 5 off-springs rats in each group randomly after birth on 7, 14, 21 and 28 day respectively and by using immunohistochemistry, detect the NMDAR1 protein expression levels in the regions of pallium, diencephalon and hippocampus.

分别在子鼠出生后第7、14、21、28天每组随机抽取5只子鼠，免疫组化染色测定子鼠大脑皮层、中脑、海马NMDAR1受体蛋白的表达。

Advancing notable test to all variables according to data of collection before using to method of stepwise discriminatory analysis to set up discriminant function.

把101例恶性肿瘤患者按中医临床辨证标准分为偏虚组、偏实组、虚实并重组，并设对照组100例，分别测定其外周血载脂蛋白A1、B100水平及A1/B100比值和免疫指标CD3、CD4、CD8、CD4/CD8比值、NK细胞及IgG、IgM、IgA，收集所得数据资料，先将各指标进行显著性检验，后运用逐步判别分析法建立判别函数对其进行判别分析。

Methods The serum level of MMP-9 in 40 patients with IPF and 30 healthy subjects was measured by ELISA.

采用酶联免疫吸附法对40例特发性肺纤维化患者和30例健康人血清基质金属蛋白酶-9 进行测定。

Methods Serum levels of MMP-9,endostatin and VEGF were detected by ELISA method in 144 patients with lung cancer.

方法应用酶联免疫吸附法测定3种因子在144例肺癌患者血清中的表达。

Characterization of the immunoreactiity measured with these assays was performed using gel filtration of human pancreatic juice before and after actiation of trypsinogen with enterokinase.

通过分别在胰蛋白酶被肠激酶激活前后对人胰液的凝胶滤过来定量测定这种免疫反应的特点。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷