免疫测定

Objective In order to understand the role of the immune function in the pathogenetic mechanism of multiple sclerosis. Method Antigangliosides GM1 antibody and cephalin antibody in serum (n=28) and cerebrospinal fluid (n=18) were examind in MS patients with ELISA assay.

目的 探讨多发性硬化的免疫发病机制方法采用ELISA方法测定多发性硬化患者活动期血清(28例)和脑脊液(18例)的GM1抗体、脑磷脂抗体和髓鞘碱性蛋白。

Objective: To study osteogenic capability of rabbit bone marrow stromal cells and biological characteristical identification in vitro in order to explore the appropriate methods of osteoblast culture in vitro and to select an idea source of seed cells for bone tissue engineer.

目的：研究兔骨髓基质细胞在体外培养条件下的成骨能力及生物学特性，探讨简便易行的成骨细胞体外培养方法，为组织工程选择理想的种子细胞来源。方法：取新西兰白兔骨髓液经离心后得骨髓单个核细胞，以1× 106／ml的细胞浓度进行培养，经传代培养，选择条件培养液培养(1640完全培养液中加入地塞米松10-8Smol／l，β-甘油磷酸钠10mmol／l，VitC 50μg／ml)作为骨髓基质细胞增殖和成骨的调节因子，通过倒置显微镜、HE染色、扫描电镜、碱磷酸酶(alkaline phosphatase，ALP)测定、Ⅰ型胶原免疫组化染色和四环素荧光钙染色等手段对获得的细胞进行生物学特性研究。

Plasma motilin, somatostatin and cholecystokinin were determined by radioimmunoassay.

运用放射免疫同批测定血浆胃动素、胆囊收缩素和生长抑素水平。

Methods: 1. To detect the GC level in the serum by enzyme immunoassay kit.The serum samples were obtained from 22 psoriatic patients, 15 patients who had been clinical cured and 20 normal controls. There were standard, control, and sample wells. The standardN control and serum samples were pipeted into the appropriate wells and then the enzyme conjugate solution, GC antiserum were pipeted into each wells, incubated the wells at room temperature for 45 minutes, washed and dried each well, added TMB chromogen solution, incubated the wells for 15 minutes, added H2SO4 to stop the reaction.

1。酶免疫法测定银屑病患者治疗前、后血清GC水平的变化：血清标本来自22例治疗前银屑病患者、15例临床治愈的银屑病患者第四军匝大学硕＿］，学位论文和 20名正常人对照，设置标准品孔、对照品孔和样本孔，标准品、对照品和血清样品分别加入对应各孔中，在各孔中再加入酶联GC，兔抗GC血清，室温下放置 45分钟后清洗吸干，加 TMP色原，15分钟后 11p。

The changes of MDA in the plasma, CINC/IL-8 and NO in the serum, MPO in the skeletal muscle were studied at the end of ischemia and 1h, 2h, 4h, 8h, 12h during reperfusion.

分别于缺血4h末，再灌注1、2、4、8、12h，测定各组血浆中的MDA、骨骼肌组织中的MPO，血清嗜中性粒细胞化学趋化因子(CINC／IL-8)，血清一氧化氮，用流式细胞仪及免疫组化法观察各组白细胞上粘附分子CD11b／CD18和骨骼肌血管中ICAM-1的表达情况及各组的组织学改变。

However, 35％ of the Chinese-Holstein milk samples failed to coagulate.

用放射免疫法测定了全奶麦洼牦牛和半奶麦洼牦牛血浆中的激素含量。

Methods: colorimetry was applied to quantitative analysis.

方法用比色法测定静注人乙肝免疫球蛋白中麦芽糖的含量。

Ursolic acid, which has immunity enhancement in the Cornus, was chosen as primary index in this research. The method of reversed-phase high performance liquid chromatography with ultraviolet detector was developed to determine the content of ursolic acid. The L9(34) orthogonal experimental design was used for Cornus to further process and explore its health product using the content of ursolic acid and the rate of paste as the assessment indexes.

本研究以山茱萸中具有增强免疫功能的熊果酸为主要考察指标，采用反相高效液相色谱-紫外检测法作为熊果酸含量的测定方法，以出膏率为参考指标，采用L9(34)设计正交试验，对山茱萸药材进行了深加工，开发其保健品；对所得到的提取物中的多糖进行了定性鉴定。

AIM and METHODS : Immunohistochemical technique in combination with a computer assisted TV-image analysis system were used to measure the expression level of c-erbB-2 and bcl-2 proteins in 37 cases of lung squamous carcinoma and 48 cases of lung adenocarcinoma identified pathologically. The expression of c-erbB-2 and bcl-2 genetic proteins in lung carcinoma and some image signs of invasiveness of lung carcinoma were analysed correlatively.

摘 要 目的和方法：利用免疫组化技术LSAB法和计算机图像分析系统的结合，测定经手术病理证实之37例肺鳞癌及48例肺腺癌组织中c- erbB -2及 bcl -2基因蛋白的表达水平，分析其表达或共表达与反映肺癌侵袭力的影像征象的关系。

Methods Exerting backwards force on rats mandible and measuring concentration of rats' blood cAMP in three groups with radio-immunity. Drawing the time-concentration curve and making cosine analysis.

给大鼠下颌戴向后上牵引的矫形器，用放射免疫法测定不同加力组大鼠血中环磷腺苷含量，绘制时间-浓度曲线，并进行时间生物学的余弦统计分析。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷