免疫测定

Immunohistochemical staining during different stages in ameloblast odontoblast and enamel free area cells were measured by the image analysis,the date of which was handled with SPSS11.5 software.

随后做图像分析测定不同发育阶段的成釉细胞、成牙本质细胞、无釉区细胞的免疫表达水平，所得数据用SPSS11.5 软件进行统计分析。

The tests show that said antihuman VEGF ScFv is small in molecular weight, possesses strong power for penetrating tumor ittue, its immunogenicity is low, so that it possesses the tumor-resisting function.

最终获得ScFv的高效表达，经测定证实抗人VEGF ScFv分子量小、对肿瘤组织穿透力强，免疫原性低，能阻遏肿瘤血管生成的抗肿瘤作用。

Methods:180 HCV patients and 40 healthy controls were selected for the research, and their serum BAFF levels were detected by ELISA. The level of serum HCV-RNA in HCV patients was detected through fluorescent quantitative polymerase chain reaction and analyzed along with the clinical data of HCV patients.

选取180名HCV感染者及40名健康对照，应用酶联免疫吸附法测定血清中BAFF含量，荧光定量聚合酶链反应检测HCV感染者血清中HCV-RNA的水平，并结合HCV感染者临床情况分析。

The expression of Bcl-2/Bax protein were determined by immunohistochemistry.

并用免疫组化法测定心肌细胞的Bcl-2和Bax蛋白的表达。

Intestine, lung, heart, kidney and liver tissue samples were also collected after reperfusion 2 hours, and (1) protein concentration of bronchia alveolus lung fluid ,(2)HSP70 expression in heart and kidney (3) histology pathology and (4) oxidation injury (MDA and GSH-PX) in above tissues were determined.

休克2小时，收集小肠、心肌、肺、肝、肾等组织用于测定(1)支气管肺泡灌洗液的蛋白含量(2)免疫组化检测心脏和肾脏HSP70的表达(3)组织病理切片(4)组织抗氧化指标。

Methods The experimental TBI model was established by bumpiness of free falling body according to Feeney′s.The rats′cerebral edema were imaged with magnetic resonance image,The changes of brain water content and permeability of blood-brain barrier were measured by the methods of wet and dry weight and Evans blue fluorometry.The expression of AQP-4 mRNA was examined by reverse transcriptase polymerase chain reaction.

按照改进的Feeney自由落体撞击法建立大鼠创伤性脑损伤模型，用磁共振成像对大鼠脑水肿进行检测，干/湿比重法和伊文思蓝测定法观察大鼠TBI后不同时相脑组织含水量和BBB通透性的变化，并采用逆转录聚合酶链反应法检测AQP-4 mRNA的表达，蛋白质免疫印迹法检测AQP-4蛋白的表达。

The eliminated rate of carbon granule and the content of hemolysin in serum in low-immune mouse were measured to explore the effect of Yi Shen Granule on immune ability.

采用免疫力低下小鼠进行碳粒廓清试验和血清溶血素含量测定试验，确定药物对机体免疫功能的影响。

The carboxyhemoglobin levels of in-flowing pulmonary blood and out-going pulmonary blood were determined at 2,4 and 6 hours after treatments. Malondialdehyde contents and superoxide dismutase activities in the lung,liver and blood were also determined. Pathological changes in lung and liver were examined with light microscope,and immunohistochemical technique was used for analysis of heme oxygenase-1 (HO-1) protein expression and distribution in lung and liver.

各组分别在制模后2、4和6 h测定出入肺血中碳氧血红蛋白水平；肺、肝组织及血液中丙二醛含量及超氧化物歧化酶活性；光镜下观察肺、肝组织形态学改变；免疫组化分析血红素加氧酶-1(HO-1)在肺、肝组织中的蛋白表达和分布。

Abstract］ Objective To investigated the cellular distribution and quantitatively of CEA in cerebrospinal fluid meningeal carcinomatosis cells by immunofluorescence staining and laser scanning confocal microscopy, explore the diagnostic value of the examination in the patients with meningeal carcinomatosis.

目的 联合应用免疫荧光细胞化学技术和激光扫描共聚焦显微镜技术检测脑脊液中脑膜癌细胞CEA的表达部位并定量测定其荧光含量，探讨该技术在脑膜癌病诊断中的价值。

Methods We measured plasma angiotensin II and aldosterone by radioimmunoassay in 40 hemodialysis patients and 20 normal controls, and the results were compared with the changes of ultrasound cardiography.

用放射免疫法测定天津市人民医院肾内科40例血液透析患者和20例健康对照组的RAAS活性并进行比较，同时将两组的RAAS水平与心脏超声指标进行相关分析。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷