免疫测定

Objective To assess the clinical significance of detection of immunosuppressive acidic protein in patients with acute leukemia.

目的 探讨免疫抑制酸性蛋白测定在急性白血病病人中的临床意义。

Rt-PA(5mg/kg) dissolve bolt gives treatment after the 3h that be short of blood, associated group gives vein injection at the same time Yidala is acted according to (the 24h after 3mg/kg) dissolve bolt undertakes nerve function grading and cerebral stalk die concentration percentage by volume leads an analysis, determine blue content of the thread of ideas in writing of the Yi in cerebral tissue, with immune organization chemical method evaluates dissolve bolt afterbrain to organize the change of hematic head protective screen.

缺血3h后给予rt-PA(5mg/kg)溶栓治疗，联合组同时给予静脉注射依达拉奉(3mg/kg)溶栓后24h进行神经功能评分和脑梗死体积百分率分析，测定脑组织中伊文思蓝含量，用免疫组织化学方法评估溶栓后脑组织血脑屏障的变化。

The contents of Riboncules acid, 5 -hydroxyptamine (5-HT), Cyclic adenosine monophosphate , Cyclic guanosine monophosphate in milk; and the activites of Alkine phosphatese , lactic dehydrogemase in serum were determined by Chromometry and radioimmunoassay.

采用比色法和放射性免疫法测定了西农莎能奶山羊乳中RNA，S-HT，CAMP，CGMP和血清中LDH，AKP的含量为：0.432，0.408，0.405，0.473，0.194，0.405。

And then HepG2.2.15 cellswere cultured with different non-cellulotoxic concentration ofα-amanitin or 3TC for six days.

采用MTT法检测不同浓度α-鹅膏毒肽对体外培养的HepG2 2.2.15细胞株的毒性，然后在无毒浓度下用不同浓度α-鹅膏毒肽或拉米夫定与HepG2 2.2.15细胞共培养6天，分别在第3天和第6天收集细胞培养上清液，采用时间分辨免疫荧光法测定上清HBsAg、HBeAg浓度；收获细胞提取总RNA，行逆转录聚合酶链反应检测细胞内HBsAg和HBcAg mRNA的表达。

The bioactivity of bFGF released from PLGA microspheres are higher than from PELA.(2) the bFGF released from PLGA microspheres can stimulate mitosis, proliferation, and matrix synthesis of chondrocyte more effectively than free bFGF.

游离bFGF对软骨细胞的促有丝分裂的作用效应期短；bFGF缓释微球能通过持续释放活性bFGF，在较长时期内促进软骨细胞的分裂增殖；Ⅱ型胶原免疫荧光测定和蛋白多糖阿新兰染色测定显示，应用微球10天后，能有效的促进软骨基质成分的合成，其效果与持续应用游离bFGF的效果相当。

Breeding wheat cultivars with low deoxynivalenol content is an effective approach to control the toxin contamination in wheat grains caused by wheat scab. In this paper, two methods for the determination of DON content in wheat grains, DON test-HPLC and GC-MS, were compared.

为选育赤霉病毒素含量低的小麦品种，减轻赤霉病危害，在比较了免疫亲和柱液相色谱法(DON test-HPLC)和气质联用法测定小麦中毒素含量的特点的基础上，利用两种方法对自然发病和赤霉病菌人工接种的小麦籽粒DON含量进行测定。

Crohn's disease was induced by trinitrobenzene sulfonic acid in the model group. The mice were sacrificed 5 days afterwards and LPMC were isolated and cultured. The expression of CCR2 and CCR5 on LPMC were determined by flow cytometry. The secretion of MIP-1α in the supernatant was determined by enzyme-linked immunosorbent assay, and the expression of MIP-1α mRNA was determined by reverse transcriptase polymerase chain reaction.

模型组小鼠以三硝基苯磺酸灌肠造模。5d后处死，提取和培养LPMC，采用流式细胞仪测定小鼠LPMC中趋化因子受体CCR2、CCR5的表达；采用酶联免疫吸附测定检测培养上清液中MIP-1α的分泌；采用逆转录聚合酶链反应检测MIP-1α mRNA的表达。

Hypoxia was imposed to the cardiomyocytes with or without daidzin pretreatment. ALDH2 activity was measured by the method of acetaldehyde metabolism. Apoptosis was measured by Hoechest 33324 staining, fluorescence activated cell soiling and the DeadEnd fluorometric TUNEL system.

运用缺氧模型，比较大鼠心肌细胞在单独缺氧和经ALDH2特异性抑制剂daidzin预处理24h后缺氧的凋亡改变，酶活性检测采用乙醛代谢法、凋亡的测定通过用Hoechest 33324、免疫荧光标记用流式细胞仪测定和TUNEL试剂盒检测。

The blood concentration of FK506 was measured by enzyme immunoassay technique.

FK506血药浓度的测定采用酶联免疫分析法测定。

Methods:In patients with ACS(n=32) and controls(n=21) the selective coronary arteriography was performed and the coronary artery stenosis scores were evaluated through accumulative total integration. Plasma concentrations of fibrinogenwere determined with hemagglutinin analysator and plasma concentrations of von Willebrand factor、 D-Dimer、 plasminogen activator inhibitor、platelet membrane glucoprotein(GMP-140) were determined with enzyme linked immunosorbent assay.

选择ACS患者32例和对照组21例进行选择性冠状动脉造影，对冠脉造影结果采用累计积分法进行冠状动脉狭窄评分；使用血凝分析仪测定受试者血浆纤维蛋白原水平，使用酶联免疫法测定外周静脉血血管性血友病因子、D-二聚体、纤溶酶原激活剂抑制物、血小板膜糖蛋白140（GMP-140）的含量。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷