免疫测定

He way of observing the level of TNF-αand IL-2 : Take blood samples of patients from peripheral vei n one day before operation and on first, third, seventh, twelfth day after opera tion; Extract serum, then measure the level of TNF-αand IL-2 by Radioimmunoassa y according to specification for reagent-box.

NF-α和IL-2含量测定：抽取正常对照组及食管癌患者术前1天和术后1、3、7、12天清晨空腹外周血，分离血清，用放射免疫的方法按试剂盒说明书进行测定。

Enzyme-linked immunosorbent assay and coagulating time method were used to assess antigenic stability and coagulating stability,respectively.

以酶联免疫吸附法测定了蛇毒降纤酶的抗原活性稳定性，并与凝固时间法测定的生物活性稳定性进行了比较。

Enzyme-linked immuno-sorbent assay and coagulating time method were used to assess antigenic stability and coagulating stability, respectively.

以酶联免疫吸附法测定了蛇毒降纤酶的抗原活性稳定性，并与凝固时间法测定的生物活性稳定性进行了比较。

This study paid more attention to the techniques of detecting Soybean mosaic virus in dry soybean seed and Bean common mosaic virus in dry bean seed by reverse transcription-polymerase chain reaction, combining with biological test and enzyme-linked immosorbent assay. The goal of the research is to establish a molecular detection procedure of SMV and BCMV from dry seed. The main results are following:⑴The effective techniques were made to extract total RNA of SMV from dry soybean seed and BCMV from dry bean seed.

本研究以大豆和菜豆种子中主要种传病毒大豆花叶病毒、菜豆普通花叶病毒作为检测对象，在生物学测定和酶联免疫吸附测定的基础上，研究干种子中两种病毒的逆转录-聚合酶链式反应检测方法，以建立一套快速、灵敏、专化性强的直接以干种子作为检测对象的分子检测技术体系，主要结果如下：⑴建立了从大豆和菜豆干种子中提取SMV、BCMV总RNA的有效方法。

METHODS: Fasting serum levels of IL6, IL8, TNFα were measured in 30 cases of T2DM with insulin resistance and 30 cases of T2DM without insulin resistance and 26 normal control subjects by enzymelinked immunosorbent assay.

采用酶联免疫吸附法测定T2DM伴有IR患者30例和不伴有IR患者30例及正常对照26例的空腹血清白细胞介素6（IL6），白细胞介素8（IL8）及肿瘤坏死因子的浓度，并同时测定空腹血糖、糖化血红蛋白（GHbA1c）及空腹胰岛素水平，分析其与IL6，IL8，TNFα的相关性。

Twelve L5 left dorsal root ganglions on each time point were harvested from sham group on the 7th day after operation and from model group on the 7th,14th and 21st day after operation and then were tested histologically by Hematoxylin-Eosin staining and the proportion of Cycloxygenase-2（COX-2） and p-p38MAPK positive expression cell were tested by immunohistochemistry method either.

测量各组大鼠术前至术后21d时左后肢50％机械性撤足阈值（50%PWT）以测定机械痛敏的变化，假手术组术后7d及模型组术后7d、14d、21d取左L5 DRG分别进行HE染色观察组织学变化和免疫组化法测定环氧化酶-2（COX-2）与p-p38MAPK的阳性细胞比率。

Capture ELISA method was used to determine isotypes of the mouse monoclonal antibody and the indirect ELISA method was performed to determine the titers of McAbs.

捕获ELISA法测定小鼠免疫球蛋白亚型，间接ELISA法测定腹水效价；用两步硫酸铵法和G蛋白亲和层析法来纯化腹水。

We collected venous blood of 4 millilitre from all the children,and quantitative determination blood LPS, LBP, mCD14 by limulus reagent kinetic turbimetric assay, flow cytometry technique and ELISA method.

所有SIRS患儿及正常儿童各取静脉血4ml，分别利用鲎试剂动态比浊定量测定法、流式细胞分析技术、酶联免疫试验进行血液内毒素、mCD14、LBP的定量测定。

Subjects who had taking iodine-containing preparations or thyroid hormone were also excluded.2.2 MethodsSerum levels of triiodothyronine ( T3 ), thyroxine ( T4 ), free triiodothyronine ( FT3 ), free thyroxine ( FT4 ) and thyrotropin were determined by immunochemiluminometric assay for ninety-fifth patients with atrial fibrillation and one hundred and thirty nine controls matched for age and sex.2.3 StatisticsAll statistical procedures were performed with SPSS 10.0 software package.

同时根据甲状腺功能指标的测定把心房颤动患者分为①正常组：TSH、FT3和 FT4水平均正常②甲状腺功能亢进组：TSH水平降低，FT3和 FT4水平升高③亚临床甲状腺功能亢进组：TSH水平降低，FT3和 FT4正常。2.2方法入选者于清晨空腹静脉取血 2 ml，采用免疫化学发光法分别测定血清中的三碘甲状腺原氨酸（T3）、甲状腺激素（T4）、游离三碘甲状腺原氨酸（FT3）。

The bioactivity of bFGF released from PLGA microspheres are higher than from PELA.(2) the bFGF released from PLGA microspheres can stimulate mitosis, proliferation, and matrix synthesis of chondrocyte more effectively than free bFGF. 10 days later after the administration of PLGA microspheres, the effects of stimulus to synthesis of type Ⅱ collagen and proteoglycan can be compared with continue administration of free bFGF.

游离bFGF对软骨细胞的促有丝分裂的作用效应期短；bFGF缓释微球能通过持续释放活性bFGF，在较长时期内促进软骨细胞的分裂增殖；Ⅱ型胶原免疫荧光测定和蛋白多糖阿新兰染色测定显示，应用微球10天后，能有效的促进软骨基质成分的合成，其效果与持续应用游离bFGF的效果相当。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。