免疫测定

Methods:The experimental group was subdivided into the bleeding and the nonbleeding groups which consisted of 64 women with IUCD.There were 60 healthy women in the control group.

采用酶联免疫吸附试验测定 64例置器妇女(试验组，试验组又分为置器出血组和无出血组)，以及60例健康妇女血清中 IL- 6水平，同时测定两组血清中 C-反应蛋白水平。

Methods Diabetic rat models were prepared by giving alloxan. After administration of sodium orthovanadate for seven days, the blood glucose levels of rats were determined. The glucose tolerances of diabetic rats were evaluated by the oxidation-enzyme method, and the insulin levels were determined by radioimmunoassay.

建立四氧嘧啶诱导的糖尿病大鼠模型，对正常大鼠及糖尿病大鼠给予原钒酸钠治疗7天后，采用葡萄糖氧化酶法测定大鼠的血糖值、糖耐量，并以放射免疫法测定血清胰岛素值。

Methods: 63 patients with ACVD and 26 age matched healthy volunteers were enroued. Plasmatic levels of TF and TFPI were examined with ELISA technique.

选择脑血管病急性期患者63例和健康体检者26例；采用双夹心酶联免疫吸附抗原测定法测定患者血浆中TF和TFPI的含量，与正常对照组及组间进行比较。

AMs that collected, pured and cultured with contine method were stimulated by LPS of different concentration(0μg/ml,0.01μg/ml,0.1μg/ml,1μg/ml,10μg/ml) for 60min or by 1μg/ml LPS for different time stage (0min,5min,15min,30min,60min,120min) to observe the dynamic change of NF-кB intranuclear level and NO production, from which the best concentration and time point of LPS stimulation were selected. In the study, all AMs were divided into 4 groups: control group, group stimulated with LPS, group interrupted by Cal C and group inhibited by PDTC. The following parameters were measured: NF-кB level in nuclear protein extraction of AMs detected with sandwich ELISA, Inter-nuclear transposition of NF-кB observed with immunocytochemistry staining, NO content in cell culture medium quantitied with nitric acid reductase assay, Morphologic change of AMs in apoptosis observed with acridine orange staining and fragmentation at genome DNA of AMs detected with apoptotic electrophoresis assay.

分离、纯化及培养大鼠肺巨噬细胞；以不同浓度的LPS(0μg/ml，0.01μg/ml，0.1μg/ml，1μg/ml，10μg/ml)和不同作用时间(0min，5min，15min，30min，60min，120min)分别刺激小室培养的细胞单层，观察NF-κB的核内浓度及NO合成量的动态变化，选择LPS的最佳用量和作用时间；然后分成四组实验，设正常对照组，LPS处理组，特异性PKC抑制剂阻断组，NF-κB抑制剂阻断组；收集培养的单层细胞及培养液；采用夹心ELISA法定量测定细胞核提取物中的NF-κB水平；免疫组化法检测NF-κB的核内移位变化；硝酸还原酶法测定细胞培养液中NO含量；吖啶橙染色观察凋亡细胞的形态学变化，凋亡电泳实验检测细胞凋亡后基因组DNA的断裂情况。

METHODS: Seralbumin, serum creatine, blood sugar, total cholesterol, total triacylglycerol, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol were detected after more than 12 hours of fasting.

测定过夜空腹12 h以上血白蛋白、血肌酐、血糖、总胆固醇、总三酰甘油、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇；用放射免疫法测定早晨第1次尿中白蛋白。

Methods:Human CD80 cDNA was transfected into B16 melanoma cells by lipofectin-mediated gene transfer before the expansion of tumor cells were tested by MTT method in vitro;C57BL/6 mice were inculated subcutaneously either mock-transfected B16 cells (B16-neo)or CD80-transfected B16 cells (B16-CD80),then the latency,survival times and tumor mass growth were investigated.The lymphocytes were examined for both proliferation indices and specific cytotoxic activity by MTT method and improved LDH-releasing method,after syngenic Mixed Lymphocyte tumor cultures.

通过脂质体介导将CD80基因导入小鼠黑色素瘤B16细胞后，利用SABC法检测CD80的表达；MTT法测定肿瘤细胞体外增殖能力；将肿瘤细胞接种至同源小鼠皮下后观察成瘤期、荷瘤小鼠存活期及肿瘤结节生长速度；在同源淋巴细胞肿瘤细胞混合培养后，通过测定淋巴细胞增殖指数和CTL杀伤活性检测肿瘤细胞的免疫原性。

To test the hypothesis, neontal rat cadiomyocytes Treat with heat shock(HS,42℃,2h) to induce the expression of HSP70,then teated with 0.5mmol/L H2O2. We first found H2O2 can reduced cadiomyocytes apoptosis and HSP70 only over-expression in the HS group, other two group only express HSP70 a little in the test of expression of HSP70.Then we tested the concentration of Ca2+, the [Ca2+ ] of CON was 192.224+6.654, the [Ca2+ ] of H2O2 group was 290.6918+8.922and the [Ca2+]of HS group was 214.2633+4.484.To further determine how HSP70 repaired the Ca2+ homestasis, we measured calcium transient of each group.

实验中应用MTT和流式细胞技术检测细胞的凋亡，发现损伤组的存活率明显低于其他2组，热休克组(Heat shock group，HS group)细胞存活率略低于正常组细胞；免疫组化结果表明只有在HS组才检测到阳性反应，即是HSP70的表达，其他2组均为弱阳性结果；应用离子成像技术对细胞内钙离子浓度进行测定，发现损伤组290.6918+8.922明显高于正常组192.224+6.654和HS组214.2633+4.484，HS组细胞内的钙离子浓度略高于正常组；为了进一步探讨HSP70对于ROS引起的心肌凋亡过程中钙离子的调控机制，又对各组的心肌细胞进行了钙瞬变的测定。

Methods: Leptin levels from serum and ascitic were measured in 67 patients with hepatic cirrhosis (among them complicated with SBP 32 and transudate group 35) by immunoradiometric assay, and the levels of TNF-α and IL-6 were measured by ELISA.

采用放射免疫法测定67例肝硬化腹水患者（SBP组32例，漏出液组35例）血清及腹水瘦素水平，同时采用双抗体夹心ELISA法测定血清及腹水的TNF-α、IL-6水平。

Results Eighty-four patients with pleural effusion were enrolled,among which 40 with malignant effusion,33 with infectious effusion and 11 with transudative effusion.t-PA level was higher in malig...

应用ELISA法测定组织型纤溶酶原激活物、纤溶酶原激活物抑制剂-1(PAI-1)浓度，应用乳胶增强型免疫比浊法测定D二-聚体浓度。

Six rats of each group were randomly sacrificed on day 7,14,28 and 42 respectively.The histological changes of lung tissue were studied by HE and Masson's trichrome staining.Hydroxyproline level in lung tissue was measured by digestion method.Protein and mRNA expression of transforming growth factor-β1（TGF-β1） were assayed by immunohistochemistry and RT-PCR respectively.

各组分别于造模后第7、14、28及42 d随机处死6只，取肺组织行HE、Masson染色评价肺组织病理变化，消化法测定肺组织羟脯氨酸含量，分别采用RT-PCR和免疫组化法测定肺组织转化生长因子β1（TGF-β1）的mRNA和蛋白表达。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。