免疫测定

The cytolytic activity of CTL and NK were detected by variable MTT method. The content of IL-2 and IFN-γ in serum were measured by ELISA.

取MMC处理的colon26细胞、colon26/pMKITneo细胞和colon26/4-1BBL细胞，分别腹腔免疫BALB/c小鼠，采用改良MTT法测定CTL和NK细胞杀伤活性，取血清用ELISA法测定IL-2、IFN-γ下含量。

The cytolytic activity of CTL and NK were detected by variable MTT method. The content of IL_2 and IFN_γ in serum were measured by ELISA .

取MMC处理的colon26细胞、colon26/pMKITneo细胞和colon26/4_1BBL细胞，分别腹腔免疫BALB/c小鼠，采用改良MTT法测定CTL和NK细胞杀伤活性，取血清用ELISA法测定IL_2、IFN_γ含量。

Serum IgG titers against P.gingivalis antigens were measured by enzyme-linked immunosorbent assay. IgG avidity was measured by diethylamine dissociation ELISA.

血清IgG抗体滴度采用酶联免疫吸附试验测定；其亲和力通过二乙醇胺分离，ELISA测定。

After the viruses were inoculated blindly into 9-11 days old chicken embryonated egg through five to six passages, animal artificial infection test and vaccination protection test were done.

用动物回归和免疫保护试验进行了毒株的致病性研究，用气管环组织培养对病毒进行理化特征测定、毒价测定与中和试验。

By using the assessment method of health care,the content of serum lysozyme,the thymus index,spleen index,the phagocytic function of the macrophage and the delayed type hypersensitivity were tested to analyse the immunoregulatory function of Hericium erinaceus polysaccharide.

应用保健功能的评价方法，通过对血清溶菌酶含量、胸腺指数与脾指数、腹腔巨嗜细胞吞噬功能的测定以及迟发型超敏反应，来定性分析猴头菌丝多糖的免疫调节作用；通过对血清SOD、CAT、MDA含量的测定，来分析猴头菌丝多糖的抗氧化功能。

The change of α1,2-FT activity in the cell line before and after the tranfection was confirmed by the determination of enzymatic activity. The changes of cell lipid and glucolipid, especially the change of type Ⅱ oligosaccharide, in the cell line before and after the transfection was determined by Thin-Layer Chromatography and TLC immunostaining method, respectively.

通过酶活性测定证明转染前后细胞系α1，2-FT活性的改变，采用薄层层析、薄层层析免疫染色方法测定转染前后细胞脂质及糖脂，特别是Ⅱ型寡糖的变化。

Methods: PNS was got by extracting panax notognseng with general method of alcohol circumfluence, purified with organic solvents, and separated with D101 macroreticular resin. The hematolysis rate and the immunocompetence of biology of PNS were determined by hemolysis test and experiment of splenocyte proliferation respectively.

乙醇回流法提取PNS粗提物，经有机溶剂除杂后，通过大孔树脂柱层析分离纯化；溶血性试验测定PNS作用后红细胞的溶血率；脾淋巴细胞增殖反应测定PNS的生物免疫活性。

Lavement of pulmonary alveolus of right lung bronchus, undertake left lung of; of cellular classification, computation organizes divide evenly oar to determine group of immunity of collagen content; changes a law to determine sex of STAT1, plaque source grows factor the expression of albumen of 1(PAI-1 of depressor of content of activation of PDGF and fine dissolve zymogen.

右肺支气管肺泡灌洗，进行细胞分类、计数；左肺组织匀浆测定胶原含量；免疫组化法测定STAT1、血小板源性生长因子和纤溶酶原激活物抑制剂1(PAI-1)蛋白的表达。

METHODS:The techniques of RT- PCR and Northern Blot were adopted to test the gene expression of OB- R and the radioimmunoassay was used to test the serum a nd hypothalamus leptin and insulin in order to observe the changes of serum lept in, insulin and lipide in simple obesity patients and the changes of weight,Lee' s index,somatic fat,the contents of serum and hypothalamus leptin and insulin as well as the expression of hypothalamus OB- R gene in obesity rats before and a fter acupuncture treatment.

采用RT-PCR和NorthernBlot技术测定瘦素受体基因表达水平，放射免疫分析测定血清和下丘脑瘦素和胰岛素的含量。

Methods: Using chemiluminescence assay to determine phagocytic function of celiac macrophagocyte and the erythrocyte immunization of the mouse model by reserpine.

采用化学发光测定和分析的方法，对利血平造模的小鼠进行腹腔巨噬细胞吞噬功能和红细胞免疫功能的测定。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。