免疫测定

The detection of estrin level can provide theoretical reference for the clinical hormone replacement therapy. Peri menopausal female ; Estrin level ; Radioimmunoassay

因此 ，准确的雌激素水平的测定将为临床的激素替代疗法提供理论参考。1 材料和方法1 。1 标本5 0例血标本均采自自愿到实验室接受检测的患者。1 。2 主要试剂血清雌二醇放射免疫测定试剂

Methods: With the application of the depression status inventory or the self depression scale the perimenopausal and menopausal women （ menopaused 1 - 3 years） between 40 and 55 years old in several communities and charities of the Changchun city were investigated to evaluate the symptom of depression by case - control method to test the serum progestogen, folate and serotonin of the climacteric women with depression, normal climacteric women and normal reproductive women 30 irrespectively, by means of radioimmunoassay and fluorescence method.

采用自我评定抑郁量表或抑郁状态调查问卷对长春市部分企事业单位的围绝经期及绝经后的更年期妇女进行抑郁症状的评价；采用病例对照方法，应用放射免疫测定方法和荧光法对更年期抑郁组、更年期正常组和生育年龄期正常对照组妇女各30例进行血清孕激素、叶酸、五羟色胺测定。

Methods By radioimmunoassay,we measured the contents of CGRP in mesencephalon and pituitary after reperfused 5 minute following 5 minute ischemia; reperfused 45 minute following 15 minute ischemia; reperfused 30 minute following 30 minute ischemia;and compared with controls(pseudo-operation, same time of ischemia and the same total time but only ischemia).

采用放射免疫测定方法，测定大鼠全脑缺血5 min再灌注5 min、缺血15 min再灌注45 min、缺血30 min再灌注30 min中脑及垂体CGRP含量变化，并与相同缺血时间组、假手术组及总时间相同但仅有缺血组相比。

Serum anti-mouse thyroglobulin antibodies titers of mice was determined by ELISA and the relationshi...

应用酶免疫测定法测定血清T4 值，评估甲状腺功能。

Methods : To study the therapeutic effect of Shubuhuo on 30 case of Hyperplasia of M- ammary Gland after three months,including the improving of clinical symptom and the size of adenoncus of the mammary gland.The Sex hormones of lood was evaluated by radioimmunoassay,including serum estrogen(E2)、progestogen、prolactin,T-cell tubgroup(CD3、CD4) was evaluated by flow cytometry,to find the diffence between pretherapy and post-treatment in luteal.

运用疏补活汤治疗30例乳腺增生病患者3个月(1个疗程)后，观察其临床疗效，包括治疗前后患者临床症状的改善以及乳腺近红外线扫描确定乳房肿块大小的变化；在治疗前后于患者黄体期用放射免疫测定法检测血清性激素水平的变化，包括雌二醇（estrogen，E2）、孕激素（progestogen，P）、泌乳素（prolactin，PRL），同时用流式细胞仪检测治疗前后细胞免疫功能（T细胞亚群CD3、CD4）的变化。

Methods: A dual-chamber culture system was prepared. Porcine ovarian follicles were divided into healthy and atretic groups. Granulosa and thecal cells were isolated, and cultured with gonadotropin in the dual-chamber system. After 48 hours, angiotensin II, renin activity, estrodiol(E2), progesterone and testosterone in the media were determined by radioimmunoassay.

制备人胎盘羊膜双池培养系统，分离了猪卵巢颗粒细胞和卵泡膜细胞，并分为健康和闭锁两组，在促性腺激素刺激下协同培养。48 h后应用放射免疫测定法测定两种细胞培养液中的血管紧张素II、肾素活性、雌二醇(E2)、孕酮以及睾酮水平。

Methods:The level of E2 was evaluated by Progesrone Serozyme,the expression of ER was tested by Immunohistocheminal assay,ER gene expression was tested by RT-PCR in 35 cases with mammary hyperplasia of stagnation of Liver-qi type,15 cases with mammary hyperplasia of disorder of thoroughfare vessel and conception Vessel type and 30 cases of healthy people were taken as control group.

分别用磁分离酶联免疫测定法、免疫组化法和逆转录—聚合酶链反应法，检测35例肝郁气滞型乳腺增生病患者及15例冲任失调型患者和30例正常人黄体期血清雌激素水平（E2 ）、乳腺组织雌激素受体及受体基因的表达。

Methods: Bypreparing anti-human type Ⅳ collagen IgG with rabbits and utilizing sodium periodate horse-radish peroxidase-labelling anti-human type Ⅳ collagen monoclonal antibody to change diluentcomponent and concentration, we establioshed sandwich ELISA.

以免疫家兔制备抗人COLⅣ多抗包被微孔板，采用改良过碘酸钠法辣根过氧化物酶标记抗人COLⅣ单抗，改变各种稀释液成分及浓度，建立双抗夹心酶免疫测定法。

This fragment, which consists of the chicken-alpha globin gene (5′-MAR)、mouse-metallothionein-Ⅰ and human insulin-like growth factor-Ⅰ, was microinjected into male pronuclei of 468 rabbit 1-cell embryos, and injected embryos were transferred into the oviduct of 36 recipients.

为了检测转基因的表达，我们用〓标记的hIGF-1作标记抗原，hIGF-1作标准抗原与hIGF-1抗血清（美国的Susan和Underwood提供）进行竞争性免疫结合反应〓首次建立了人胰岛素样生长因子（IGF-1）的放射免疫测定方法。

Chemiluminescence Immunoassay is an efficient detection tool combined from chemiluminescent reactions and immunoassay.

化学发光免疫技术是化学发光与免疫测定结合起来的一种高效检测手段。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。