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免疫测定

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Using 90 g/L bovine serum albumin Tris buffer as determination solvent, the estradiols content in female silkworm moth could be exactly and stably measured by radioimmunoassay.

以90g/L牛血清白蛋白Tris缓冲液为测定溶剂,采用均相竞争的放射免疫法,可精确、稳定地测定出家蚕雌蛾体中雌二醇含量。

The symptoms such as cough, sputum and stridor etc of both groups who were treated by point application for two weeks were observed. the serum levels of il 2, tnf α both of the admission of hospital and after being treated by elisa method were measured in these cases.

采用酶联免疫吸附方法测定70例慢阻肺急性发作期患者入院初及治疗后血清中的白细胞介素 2(il 2)、肿瘤坏死因子α的水平,同时测定肺功能(fev 1%pre、fev1/fvc、mvv。

It was studied that Apolipoproteins AⅠand B were determined by scattering immunoturbidimetric method using fluorescence spectrophotometer in stead of laser turbidimeter,and meanwhile the reference values of apolipoproteins AⅠand B of health persons were investigated.

研究了荧光分光光度计的免疫散射比浊法代替激光散射浊度仪来测定载脂蛋白AⅠ和载脂蛋白B,同时测定正常人的ApoAⅠ和ApoB的参考值。

With a model of reversible middle cerebral artery occlusion in rats, the effects of Losartan on the water content of the brain, the contents of Evens blue and the expression of MMP9 were investigated after 24 hours of reperfusion following two hours of cerebral ischemia.

采用血管内栓线阻断法制备大鼠局灶性脑缺血再灌注损伤模型,于脑缺血2?h、再灌注24?h后测定脑组织含水量、伊文斯蓝含量,免疫组织化学方法测定MMP9表达。

With a model of reversible middle cerebral artery occlusion in rats, the effects of Losartan on the water content of brain, the contents of Evens blue and the expression of MMP-9 were investigated after 24 hours of reperfusion following two hours of cerebral ischemia.

采用血管内栓线阻断法制备大鼠局灶性脑缺血再灌注损伤模型,于脑缺血2h、再灌注24h后测定脑组织含水量、伊文斯蓝含量,免疫组织化学方法测定MMP9表达。

Methods To determine the serum PCT of 35 patients with bacteriosis and 15 patients with nonbacterial disease by gold immunochromatographic assay,and determine C-reactive protein and alkaline phosphatase of neutrophilic granulocyte in corresponding period.

方法采用半定量的胶体金免疫结合法,测定35例细菌感染性疾病住院患者及15例非细菌感染性疾病住院患者的PCT,并同期测定患者C-反应蛋白与中性粒细胞碱性磷酸酶。

The patients with bisectional prostate hypertrophy (BHP, 415 cases) and prostate cancer (PCa, 225 cases) were diagnosed by pathobiology, and whose levels of PSA, and fPSA were examined in serums by ELISA.

病理诊断为415例良性前列腺增生和225例前列腺癌,利用酶联免疫法测定患者血清中的PSA、游离PSA,利用经直肠超声测定前列腺体积,并计算出f/tPSA及PSAD进行统计学分析。

The aims of the writers study is to observe the effects of its inducing apoptosis and influencing cell cycle by treating esophageal carcinoma cases with sodium cantharidate before operation.

本研究通过术前应用斑蝥酸钠注射液,旨在观察其诱导食管癌肿瘤细胞凋亡、对肿瘤细胞周期的影响,并初步探讨两者的关系;通过测定p53、bcl-2基因表达在应用斑蝥酸钠后的变化,初步探讨斑蝥酸钠治疗食管癌的分子机制;同时测定应用斑蝥酸钠后的免疫功能变化,以期对斑蝥酸钠对机体的影响做出较全面的评价。

Methods The model of SAH was set up by injecting blood two times via cisternal after occlusion of bilateral carotid in rabbit. The food-intake and behavior of experimental animals were under observation at various stages of experiment. The content of ET in blood and CSF was tested by radioimmunoassay, and the NOx- by HNO3 redutase method. Regional cerebral blood flow was measured by hydrogen clearance method.

方法采用双侧颈动脉结扎和枕大池二次注血制成兔SAH模型,观察SAH前后动物进食量和神经功能改变,用放射免疫方法和硝酸还原酶法分别测定血液和脑脊液中ET和NOx-含量,以氢清除法测定局部脑血流量。

Methods: Adenosine deaminase was purified by immtino-affinity chromatography, the enzyme activity was assayed colorimetrically by the measurement of ammonia produced over a fixed timeResults: The purified ADA was shown to be a homogeneity by polyacrylamide gel electrophoresis.

用免疫亲和层析法分离纯化人肾ADA,测氨法测定 ADA活性,经SDS-PAGE测定其分子量。

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