免疫测定

The mechanism of air pollution on health was studied by questionnaire, lung function determination, the level of serum immuniglobulin analysis, and analysis of local aerobe and anaerobe bacteria of pharynx in quality and quantity.

以小学生作为研究对象，通过进行问卷调查、肺功能测定、免疫球蛋白水平测定，以及对口咽部需氧菌和厌氧菌进行定位、定性、定量分析，探索大气污染与机体健康效应及其作用机制。

At baseline and on day 1, 3, 7, 14, 21 following MPP+ injection, the degree of microglial activation was examined by detecting the immunodensity of OX-42 in the substantia nigra. The number of viable dopaminergic neurons was determined by measuring tyrosine hydroxylase positive neurons in the SN. Behavioral performances were evaluated by counting the number of rotations induced by apomorphine, calculating scores of forelimb akinesia and vibrissae-elicited forelimb placing asymmetry.

分别在基线、MPP+注射后第1、3、7、14、21天时通过测定黑质区域OX-42的免疫荧光强度评定小胶质细胞的激活程度，测定酪氨酸羟化酶的表达情况评定存活多巴胺神经元的数量；通过阿朴吗啡诱导的旋转行为、前肢跨步及触须引发的不对称放置试验得分测评行为学表现。

Methods:To produce antihuman DR5 monoclonal antibody(mDRA6) by immunizing BALB/c mice using DR5 protein. The change of DR5 expression after DDPtreated on HL60 cells was detected by FACS. Morphologic changes of HL60 cells were observed under fluorencence microscope. Cytotoxic and apoptotic effects of mDRA6 and DDP on HL60 cells were measured by MTT analysis. DNA fragmentation was detected by agarose gel electrophoresis.

DR5蛋白免疫BALB/c小鼠，融合筛选抗DR5杂交瘤细胞，制备抗DR5单抗——mDRA6；流式细胞术测定顺铂对HL60细胞表面DR5表达及细胞凋亡率；荧光显微镜下观察mDRA6与顺铂协同作用下HL60细胞形态变化；MTT法测定不同浓度的顺铂与mDRA6对HL60细胞存活的影响；琼脂糖凝胶电泳检测mDRA6与顺铂联合对HL60细胞DNA片段化的影响。

The immunosuppressive effect was determined with ratio of lymphocyte blastogenesis,phagocytotic function of macrophage,percentage of peripheral T-lymphocyte,delayed type hypersensitivity and antibody forming cell assay.

免疫抑制作用：测定小鼠脾淋巴细胞转化率；巨噬细胞的吞噬功能；外周血T淋巴细胞的百分率；迟发性的变态反应；血清溶血素和抗体形成细胞功能测定。

The method uses radiative immunity law to determine CV group, healthy group and content of the SIgA in taking secretion of bacterium group vagina, method of the conglutinate outside using system determines white beads bacterium is epithelial to the vagina (the conglutinate ability of VEC), the influence of VEC of conglutinate of white beads bacterium when observing SIgA standard.

方法采用放射免疫法测定CV组、健康组及带菌组阴道分泌物中sIgA含量，用体外粘附方法测定白念珠菌对阴道上皮细胞的粘附能力，观察sIgA水平时白念珠菌粘附VEC的影响。

The liver fibrotic model was induced by CCl4 i.p for 5wk. Telmisartan was given at the same time model establishment in each intervened group by administration lasted for 6wk. Decollate to get the blood of all rats after 7 weeks . ALT、AST and ALB of serum was detected by automatic biochemistry equipment. Hyaluronic acid was detected by ELISA detection. Liver and spleen was obtained to calculate the index. The liver pathological changes were observed in each group of rats after HE staining to evaluate the therapeutic effect of Telmisartan; TGF-β1、PPAR-γand PDGF of liver tissue was detected by the method of immunohistochemistry respectively; The mRNA expression of TGF-β1、MMP-1、TIMP-1、α-SMA、PPAR-γand iNOS was detected by RT-PCR detection, respectively.

腹腔注射CCl4制备SD大鼠肝纤维化动物模型，连续灌胃6周，处死动物取血，检测血清ALT、AST、ALB；ELISA法测定血清透明质酸；取肝、睥，并计算肝、脾指数，组织切片HE染色后进行组织病理学疗效评估；用SP免疫组化法测定肝组织TGF-β1、PPAR-γ及PDGF蛋白表达；半定量RT-PCR方法检测肝组织TGF-β1、MMP-1、TIMP-1、α-SMA、PPAR-γ和iNOSmRNA的表达。

Histopathological changes of lung tissue were observed under light microscope. The protein expression of MMP (MMP-2, MMP-9) and TIMP (TIMP-1) in lung tissue were determined by immunocytochemistry. RESULTS: Decompensate respiratory acidosis (pH =7.20±0.04, PaCO2 =7.84±0.15) developed in group B.

用ELISA法测定大鼠血清MMP-2、MMP-9、TIMP-1活性及NE活性；弹力纤维染色标本进行弹性纤维相对含量的测定；免疫组化方法检测肺组织中MMP-2、MMP-9及TIMP-1的表达及光镜下进行病理学观察。

We discussed the test methods on melatonin, and tested different behavior development worker's melatonin in Apis cerana cerana Fabricius by RIAmehtod.

本文探讨了褪黑素的测定方法，并应用放射免疫法对不同行为发育和表现的中华蜜蜂的褪黑素进行了测定。

Objective To explore the clinical significance of changes of serum IL-4 and IL-10 in patients with Graves' Disease.

目的：探讨IL - 4、IL - 10水平变化在甲亢的临床意义。方法：选择甲亢未治疗组6 4例、甲亢经1 31 I治疗组39例和正常对照组35例，用化学发光法测定FT3、FT4 、TSH的含量，用放射免疫分析测定IL - 4、IL - 10含量。

Methods: The changes of surum-level T 3,T 4,FT 3,FT 4 and TSH in examinee by RIA were observed.The blood glucose were observed by methods of hexokinase.

用放射免疫法测定受试者血清 T3、T4、FT3、FT4、TSH的水平，用己糖激酶法测定血糖。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。