免疫测定

The changes of behavior were recorded in every week. The contents of adrenocorticotrophic hormone, TSH, T3, T4 and rT3 in the serum were detected by radioimmunity.

每周测定各组大鼠行为学的改变，治疗结束后用放射免疫法测定大鼠血清ACTH，T3，T4，rT3和TSH的浓度。

The cases of the two groups,their peripheral blood TNF-α and Fn Levels,in the 1st and 7th days of their life,were assayed and comparatively analyzed by a double-antibody sandwiching enzyme-linked ...

分别用双抗体夹心酶联免疫法与免疫透射比浊法于生后第 1天、7天测定其外周血TNF α、Fn含量，并作对比分析。

The ulnas of fetal mice were cultured for 48 hours in BGJb medium treated respectively with estradiol and purearin. After fixing long bones and decalcification, paraffin sections were made, and then performed immunohistochemical staining for determining the level of typeⅠcollagen protein in growth plates. Positive cell locations in the resting zone, proliferative zone and hypertrophic zone of epiphyseal plate were observed under light microscope.

剥离出胎鼠尺骨，置于BGJb培养基中，葛根素和雌二醇作用48 h后，将长骨固定，脱钙后作石蜡切片，进行免疫组织化学染色，光镜下观察骺板静止区、增殖区和肥大区阳性细胞定位，图像分析系统下测定免疫反应阳性细胞面积。

From the first experimental week, nine geese per groupwere weighed on an empty stomach ,and collected samples for further assess on 7th in every week;From the third experimental week, excepting for weight, three geese per group were randomly selectedand collected blood in jugular vein, then separated serum and assessed blood, endocrine andimmunological index; after collecting, geese were killed and autopsied, Spleen, bursa of Fabricius andThymus were collected for relative weight of immune organs of goose, calculated immune organs index.

从试验第一周开始，每周的第7日每组随机选取9只鹅空腹称重；统计耗料量；从试验第三周开始除称重外，每组再随机选取3只，颈静脉采血5ml，制备血清并测定血液、内分泌和免疫指标；采血后宰杀取脾脏、腔上囊和胸腺，称重，计算免疫器官指数。

The monoclonal cells were selected using cloning rings. The expression of capsid protein was detected by indirect immunofluorescence and sandwich-ELISA. The empty capsids of FMDV were observed under electron microscope.

经间接免疫荧光和酶联免疫吸附测定（Enzyme-linked immunosorbent assay，ELISA）方法检测MDBK细胞中衣壳蛋白的表达，并在电镜下观察口蹄疫病毒空衣壳。

Immunohistochemical identification of cultured conjunctival cellsWhen the second cultured generation of conjunctival cells close to confluence, the glass coverslips were removed in situ fixation by methanol and DAB color immunohistochemical staining by CK13 monoclonal antibody in time.3. growth curve of conjunctival cellsThe 1st,3rd,5th passage cells were selectecd randomly and subcultured at the density of 5×10~4/ml,after culturing for another 1-9 days, were counted and compared the cell number every day.

采用消化后组织块法培养结膜原代细胞，并进行传代。2、培养结膜细胞的免疫组化鉴定把第2代的结膜细胞接种于置在培养皿中的盖玻片上，当细胞接近融合时，及时取出作原位甲醇固定，CK13单抗、DAB显色免疫组化染色。3、结膜细胞生长曲线的测定随机选取第1、3、5传代细胞，以5×10~4／ml密度传代、分别培养1-9天，每天分别细胞计数并进行分析比较，重复3次。

Objective To explore the significance of the determination of Ferritin in ECLIA and RIA.

摘 要］目的：评价电化学发光免疫分析法和放射免疫分析法在测定血清铁蛋白中的应用。

Methods: The study was designed to observe SS and influence of serum CD_4~+、CD_8~+、IL-2、IL-6、TNF-α, splenic lymphocyte transformation rate, the expression of SS、SSTR-2 in spinal cord、PAG、NRM and hypothalamus the coexistence of SS and enkephalinergic neuron in back corner of spinal cord, the coexistence of SS and 5-HT neuron in NRM by using methods of behavioral nociceptive threshold test, measure of foot's cubage, flowcytometry, MTT, radioimmunity, HPLC-ECD, immunohistochemistry, laser confocal microscope on FAC-induced hyperalgesia.

以弗氏完全佐剂诱发的炎症痛模型大鼠为研究对象，应用行为学痛阈观察、足跖容积测定、流式细胞检测法、MTT法、放射免疫法、高效液相电化学法、免疫组织化学技术法、激光共聚焦技术等方法，观察SS和电针对痛阈、大鼠CD_4~+、CD_8~+、IL-2、IL-6、TNF-α水平和脾淋巴细胞转化率等指标的影响，以及SS、SSTR-2在脊髓和PAG、NRM及下丘脑部位的表达、在脊髓SS与脑啡肽能神经元的共存现象及在NRM中与5-HT能神经元的共存情况。

Methods LN in mouse endometrium was shown and measured with indirect immunofluorescence techniques, immunohistochemical ABC method and image analysis system. The effect of LN on the implantation was studied by using LN-antibody intra-uterine injection.

应用间接免疫荧光法、免疫组织化学ABC技术及图像分析法进行LN定位和定量测定；利用子宫内注射LN抗体的方法探讨LN对小鼠胚泡植入的作用。

Methods Phosphotyrosine was first combined with hemocyanin from keyhole limpets. Then, rabbits were immuned with the complete antigen and antisera were gained. The ELISA system consisted of purified antiphosphotyrosine antibody as first antibody and sheep antirabbit lgG-HRP as second antibody. Results CV within the batch was 8.4%.

应用合成的磷酸酪氨酸-钥孔帽贝血蓝蛋白免疫家兔得到抗血清，将纯化的抗磷酸酪氨酸抗体作为第一抗体，羊抗兔Ig酶结合物作为第二抗体，建立双抗体夹心酶联免疫吸附测定法。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。