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免疫测定

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Methods Recombinant GDH was used to immunize Balb/C mice and the mAbs against GDH were prepared using hybridoma technique followed by identification of IgG isotype and its affinity.

方法用基因重组的GDH蛋白免疫Balb/C小鼠,采用杂交瘤细胞技术制备McAb,测定其免疫球蛋白亚类及其亲和力。

Methods B.melitensis 16M was used to immunize Balb/C mice and the mAbs were prepared using hybridoma technique followed by identification of IgG isotype and its affinity.

B.melitensis 16M全菌免疫Balb/C小鼠,采用杂交瘤细胞技术制备McAb,测定免疫球蛋白亚类及亲和力。

Methods: With enzyme linked immunosorbent assay and micronize strengthening immunofixation, The serum OX-LDL and HS-CRP level of 215 patients with ACI was measured on the second day after hospitalization. In addition, 30 healthy volunteers were taken as the control group.

采用酶联免疫吸附试验、微粒化增强免疫固定试验测定215例ACI患者入院第2天血清中OX-LDL、HS-CRP含量水平,并与30例健康者作对照。

Fungus polysaccharose abstracted from Cordyceps militaris was used as immunopotentiator,and was tested the effects on immune activities of the serum and lymphocyte of Penaeus chinensis .

选用从北虫草中提取的真菌多糖作为免疫增强剂,测定真菌多糖对中国对虾血清及淋巴器官中淋巴细胞的免疫活性的影响。

Fungus polysaccharose abstracted from Cordyceps militaris was used as immunopotentiator,and was tested the effects on immune activities of the serum and lymphocyte of Penaeus chinensis.

摘 要 选用从北虫草中提取的真菌多糖作为免疫增强剂,测定真菌多糖对中国对虾血清及淋巴器官中淋巴细胞的免疫活性的影响。

The two kinds of polysaccharose had been compared their immune pharmacolog -ical effect by a series of tests to the mice which were immunological organ weight test, celiac mononuclear cacrophage test, erythrocyte rosette formation test, EAC rosette formation test, metatarsal swelling thickness test, and lymphocyte transformation test.

通过PPS1对小鼠进行免疫器官重量试验、腹腔单核巨噬细胞功能测定试验、E玫瑰花环试验、足跖肿胀厚度试验、T淋巴细胞转化试验、EAC花环试验,检测PPS1的免疫药理学作用。

Polysaccharide was isolated and purified from Chinese medicinal herb Ramulus mori, and determination method for Ramulus mori polysaccharide was developed in this study. Carbon clearance test and dinitrylfluorobenzene-induced delayed type hypersensitivity test were used to evaluate effects of RMPS on immune responses of mice treated with dexamethasone.

采用水提醇沉法从中药材桑枝中分离纯化多糖,并建立桑枝多糖的含量测定方法;采用碳粒廓清法和二硝基氟苯诱导小鼠迟发型变态反应试验法,观察桑枝多糖对地塞米松所致免疫低下模型小鼠免疫功能的影响。

METHODS: SERT immunoreactivity was measured using an immunoblotting procedure in autopsied brain of 16 chronic MA users testing positive for the drug in blood and brain and matched controls.

对16例血液和大脑MA检测呈阳性的MA长期使用者及与之匹配的对照者的尸体行解剖,获取脑组织,采用免疫印迹法测定SERT的免疫反应性。

Methods] Immunological analysis methods, enzyme immunology methods and latex strengthing immunological turbidimetry were used to detect H-FABP and D-D in 42 patients with myocardial infarction, 31 cases with unsolid cardiac angina, 25 cases with solid cardiac angina and 30 healthy controls.

方法] 采用酶联免疫分析法、酶免疫法测定42例心肌梗死患者,31例不稳定性心绞痛患者,25例稳定性心绞痛患者及30例健康体检者H-FABP、D-D聚体。

On the basis of purification and immunity of vitellin, antibodies of vitellin were obtained and used to measure the concentration of vitellin in embryo in different stages of embryonic development by enzyme-linked immunosorbent assay.

在采用纯化的卵黄磷蛋白免疫兔子而获得其抗体的基础上,运用酶联免疫吸附检测法(enZyme一linked ilnlnunosorbent assay,ELISA)分析测定了发育过程中胚胎内卵黄磷蛋白的含量。

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