免疫测定

In present study, zooblooting, ELISA and whole-mount immunocytochemistry methods were used to identify the FXPRLamide family neuropeptides from the Japanese oak silkworm, Antheraea yamamai.

利用Zooblotting、酶联免疫吸附测定和免疫组织化学等方法，对天蚕的DH、PBAN等FXPRLamide家族神经肽进行鉴定。

Indexes were observed to detect the effect of Se capsule on immunoregulation and antiradiation in mice.

摘 要：为研究富硒胶囊对小鼠免疫能力和辐射损伤的影响，选择了检测免疫调节和抗辐射损伤的8项指标，对富硒胶囊的功效作用进行了测定。

The peripheral venous blood was sampled before operation and on the postoperative days 1 and 7 to determine the serum levels of IgG, IgA, IgM, the percentage of T lymph cell subgroups CD3, CD4, CD4/CD8 and CRP, PGE,α- antitrypsin etc.

分别于术前、术后第3 天及术后第7 天采集外周血标本，检测免疫功能指标IgA、IgG 和IgM，采用免疫组织化学法测定T 淋巴细胞亚群及炎症指标。

The preparation of immunopeptides hydrolyzed from Cervus elaphus antler blood by papain was studied.

选用木瓜蛋白酶对天山马鹿茸血进行水解制备免疫活性肽，确定了最佳水解工艺条件，并测定了三种不同水解度水解物的抗氧化活性和免疫活性。

The morphological and cytopathological characters of the viruses were observed by electron microscopy The types of virus were identified with I-ELISA, DAS-ELISA, TAS-ELISA, RT-PCR and IC-PCR. The CP genes of the main viruses identified in this research were amplified by PCR, cloned into pGEM-T and sequenced. The pumpkin germplasm from Heilongjiang and Yunnan provinces was screened for resistance to the main viruses using friction and roots immersed inoculation.

应用电子显微镜负染法和超薄切片法观察病原粒体形态及细胞病理学特征；利用酶联免疫吸附测定法中的双抗体夹心ELISA、间接ELISA、三抗体夹心ELISA以及反转录聚合酶链式反应、免疫捕捉PCR方法鉴定了采集样品中的病毒种类；对被确定为南瓜主要病毒病原的外壳蛋白基因进行了PCR扩增，克隆到pGEM-T载体并进行测序；应用摩擦接种法和浸根接种法对云南、黑龙江省部分南瓜品系、品种进行了抗性筛选。

We examined the properties of DC associated with immune suppression in NOD mice by insulin injection subcutaneously and ability to suppress diabetes transfer by diabetogenic effect cells in secondary NOD severe combined immunodeficiency recipients.

NOD小鼠皮下注射牛胰岛素诱导免疫抑制，以血糖检测、胰腺组织学分析和胰岛beta细胞凋亡分析观察自身抗原的免疫保护作用，分离骨髓树突状细胞细胞，流式细胞术分析细胞表面抗原，混合淋巴淋巴细胞反应测定细胞功能。

Under inhibit tumor fall increment andalert confession The objective of this experiment is to prove the antitumor effect and its mechanism in vivo of Erysimum cheiranthoides. In this study we observe the ratio of inhibitory of mice bearing S180 and the effect of prolonging lives of mice bearing H22;the effect on immune function of mice bearing S180;the phagocytosis of MO cells; measuring serum TNF of mice bearing S180 with ELISA; the expression level of p53 in tumor tissue with ICH and the change of tumor cells shape with pathology test.

实验采用S_(180)、H_(22)两种瘤株，以昆明利，小鼠为研究对象，对体内的抑瘤作用、生命延长作用进行研究；对S_(180)荷瘤小鼠免疫功能影响的研究；采用碳粒廓清法研究该药对巨噬细胞吞噬功能影响的研究；采用ELISA法检测S_(180)荷瘤小鼠外周血TNF含量；采用免疫组化法测定S_(180)小鼠瘤组织中p53蛋白的含量并观察瘤细胞的病理形态。

Each group was further divided into 4 sub-groups according to the different stage of the estrous cycle.

采用放射免疫和免疫组织化学法分别测定处于动情周期不同阶段大鼠血清和牙周组织中的雌二醇含量。

AIM: To determine whether immunization with recombinant filarial chitinase or a fragment containing the epitope recognized by McAbMF1 and SXP 1 could protect jirds against microfilaremia resulting from infection with B.malayi.

目的：测定重组马来丝虫壳质酶（r－chitinase）及其片段和SXP－1抗原免疫接种沙鼠后是否具有抗马来丝虫微丝蚴血症的保护性免疫作用。

Gibel carp were inoculated with formalin killed Aeromonas hydrophilaand fed with diets supplemented with appropriate amount of yeast β-glucan. 28 days after the inoculation, the infulence of dietary yeast β-glucan on the resistance of gibel carp against Aeromonas hydrophila were determined by examing their gained weight, agglutinating antibody titer, lysozyme activity, phagocytic activity and relative percent survival by challenging with live A.

在饲料中添加定量的酵母β-葡聚糖，投喂经注射接种福尔马林灭活的嗜水气单胞菌(Aeromonas hy-drophila)菌苗的异育银鲫28 d后，通过测定供试鱼的增重量、血清中凝集抗体效价、溶菌酶活性、谷丙转氨酶活性、血清总蛋白含量、白细胞吞噬活性以及活菌攻毒后的免疫保护率，探讨了酵母β-葡聚糖对受免异育银鲫免疫应答的增强作用。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。