免疫测定

Methods The testing method of red cell C3b receptor anadem and red cell immune compound anadem created by GuoFeng were used to determine the adhesion immunity.

方法采用郭峰[1982]首创的红细胞C3b受体(RBC-C3b)花环和红细胞免疫复合物花环测试法，测定红细胞免疫粘附功能。

Experimental methods: pathological slices: To observe the changes of pathomorphology and measure the circular area of internal elastic lamina , area of neonate intima and vascular lumen, the intimal proliferatory index and percentage, the index of lumen stenosis and positive index of proliferating cell nuclear antigen through the specimens stained by HE and immunohistochemistry.

实验方法：病理切片：进行HE染色和免疫组织化学染色，分别观察病理形态学改变；测定和计算受损血管内弹力膜环绕总面积、新生内膜面积、管腔面积、内膜增生指数、内膜增生百分比、管腔狭窄指数和增殖细胞核抗原阳性指数；观察MMP-2、MMP-3、TIMP-1和PDGF-BB的免疫组化表达。

One hundred and eighty 14-days-old chickens were divided randomly into coccidia primary infection group (I1),coccidia secondary infection group (I2),and control group.Immune indexes were examined by using immune staphytococcal protein A rosette test,indirect enzyme-linked immunosorbent assay,cellular culture technique and MTT method.

将180只14日龄雏鸡随机分为毒害艾美球虫初次感染组、二次感染组和对照组，应用免疫SPA菌体花环、间接ELISA及细胞培养技术和四甲基偶氮唑盐测定法对相关免疫学指标进行了检测，以研究毒害艾美球虫二次感染对雏鸡外周血液免疫功能变化的影响。

Enzyme immunoassay was used to detect Apelin in plasma and myocardia. RT-PCR was used to analyze the relative amount of mRNA of Apelin and APJ in the myocardia.

酶联免疫法测定血浆和心肌中Apelin含量，RT-PCR法测定心肌组织Apelin、APJ mRNA水平，Western blot方法测定心肌组织中APJ蛋白水平。

The model of ECV-304 cell oxidative stress injury was established by hydrogen peroxide (H2O2).Then EPZ-contained blood serum was taken as experimental drug. The adherence of monocytes to endothelial cell were measured by method of rose Bengal. The total RNA of cells was extracted. The intercellular adhesion molecule-1 (ICAM-1),vascular cell adhesion molecule-1 (VCAM-1) and MCP-1 mRNA expression in cells were detected by RT-PCR.MCP-1 protein expression were detected by ELISA.

采用过氧化氢建立离体培养的血管内皮细胞(ECV-304)氧化应激损伤模型，取健康大鼠每日灌服不同剂量的鲜姜有效部位(200，400，800 mg·kg-1)或阳性对照药洛伐他汀(40 mg·kg-1)，取含药血清作为受试药物，用孟加拉玫红染色法测定单核细胞与内皮细胞的黏附力；逆转录聚合酶链反应测定细胞内细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子(VCAM-1)以及单核细胞趋化蛋白(MCP-1)的mRNA表达水平；酶联免疫吸附法测定MCP-1蛋白表达水平。

The test groups were drenched orally with different dosages of compound Radix scutellariae granules. After being drenched orally for ten days, their thymus-index and spleen-nidex, immune globulins of IgA, IgG, IgM and alexins of C3, C4 of serum were defermined, and experiments in vivo were done. The mice-spleen's lymphocytes which were cultured in vivo were added with different concentrations of compound Radix scutellariae granules. The lymphocytes translation stimulate indexes were measured. The ability of licking up neutral red of the peritoneal macrophages cultured in vivo was measured in the groups.

应用复方黄芩颗粒给小鼠灌胃10d后，分别测定对照组与实验组胸腺指数、脾脏指数和血清中的免疫球蛋白IgA、IgG、IgM和补体C3、C4；并且进行体外实验，用不同浓度的复方黄芩颗粒剂对小鼠脾脏淋巴细胞进行体外培养，测定淋巴细胞转移刺激指数；在体外培养测定细胞吞噬中性红的能力。

METHODS: Adjuvant-induced arthritis rats were treated with acupuncture therapy, Chinese herb treatment or both in combination for one week, then the density of TMPH in heart, liver, kidney, and hind paw was determined by HPLC. The cAMP level in the heart and liver was also measured by radioimmunoassay. The blood changes were detected through the test of blood rheometer.

以佐剂性关节炎大鼠为模型，观察单纯针刺法、单纯药物法及针药结合法分别连续治疗一周后，用HPLC法测定各组大鼠心脏、肝脏、肾脏及病灶局部组织中盐酸川芎嗪的含量；用放射免疫法测定各组大鼠心脏、肝脏中cAMP水平；用血液流变仪测定各组大鼠的血液流变学变化。

At the endpoint, the thymus, spleen and uterus were weighed separately; the femoral dry weight, bone-calcium, phosphor, magnesium and hydroxyproline contents of femoral were measured; the histomorphological features of bone were observed and the biochemical indexes in serum (Ca, P, ALP, TRAP, E2, BGP) were determined after the mice were given SAR at the dose of 10, 30, 90 mgkg^(-1) respectively, and were compared with the model group, the normal control and the positive control. RESULTS: The osteoporosis of high turnover type was formed with feeding retinoic acid to experimental mice. Compared with the model group, the lessening of femoral dry weight, the short femoral transverse diameter, the decrease of bone mineral and bone-hydroxyproline of SAR groups were improved.

维A酸105mgkg^(-1致小鼠骨质疏松模型，SAR10、30、90mgkg^(-1灌胃给药，实验过程监测体重，2周后以比色法测定血清钙、磷含量和碱性磷酸酶、抗酒石酸酸性磷酸酶活性，竞争放射免疫法测定血清中骨钙素、雌二醇(s-E2)含量，处死动物后取脾、胸腺、子宫称重，取股骨进行骨组织形态学观察，测定并比较骨计量学和骨钙、骨磷、骨镁及骨弃脯氨酸含量。

We killed some mice at the day 6th , the others were continue to expose to formaldehyde and observe their estrous cycle. HE staining was used to study the pathological changes happened in ovarian tissue. Uranium acetate and lead acetate double staining was used to study the ultrastructure of oocyte. E2 and LH level in blood serum was measured by radioimrnunoassay reagent kit.

第6d处死一批小鼠，用HE染色观察小鼠卵巢组织的病理改变，用醋酸铀—醋酸铅双重染色电镜下观察小鼠卵母细胞超微结构的损伤；用GSH测定试剂盒测定小鼠卵巢组织中的GSH含量；用E_2和LH放射免疫分析试剂盒分别测定小鼠血清中E_2及LH水平；采用超排卵法，计数超排卵数并收集卵母细胞，于油镜下观察生殖细胞遗传物质的损伤。

It has streptomycin and dihydrostreptomycin crossing rate of 100 %, simple sample pre-treatment including water dilution of milk sample and extraction with buffering phosphoric acid solution and pH regulation of pork and kidney sample, fast detection, high detection sensitivity up to 11.2 microgram/L and other advantages, and may be used in detection of residual streptomycin and similar medicines in animal food products.

本发明的检测链霉素药物的酶联免疫试剂盒为多残留检测试剂盒，链霉素和双氢链霉素的交叉率为100％；对样品的前处理要求低，样品前处理过程简单，牛奶样品用水稀释后可直接测定，猪肉和肾脏经磷酸缓冲液抽提后调pH即可上样，并且能同时快速检测大批样品；测定方法简单，省时，牛奶样品在1小时之内可得出测定结果；最低检测限达11.2μg/L，具有高特异性、高灵敏度、高精确度、高准确度等特点，可在动物性食品链霉素药物残留检测中发挥重要作用。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。