免疫测定

Methods: To weight immune organs, detect macrophage phagocytosis and hemolysin antibody production, lymphocyte transfermation and MTT.

采用免疫重量法计算脾脏和胸腺指数、巨噬细胞测定法及溶血素测定法、淋巴细胞转化实验、MTT法测定。

Immunohistochemistry technology was used to evaluated tyrosine hydroxylase of DA synthesis. DA, 5-hydroxytryptophan(5-HT) and their respective metabolic products 3,4 -dihydroxyphenylacetic, homovanillic are also precisely quantified in this research by using high-pressure liquid chromatography with electrochemical detector. The main metabolism monoamine oxidase of DA was processed too. RESULTS:WD can increase the synthesis and the metabolism process of DA .

本文观察了温胆汤及其配伍对大鼠刻板行为的影响，采用了免疫组化技术对多巴胺的合成酶—酪氨酸羟化酶进行了含量测定，应用高效液相-电化学法测定了DA、5-羟色胺（5-HT）及代谢产物3，4—二羟基苯乙酸、高香草酸的含量，并测定了DA主要降解酶—单胺氧化酶。

The rats of model group,acupointⅠgroup, acupointⅡgroup were treated with passive smoking.The rats of acupointⅠgroup,acupointⅡgroup were treated with placing Chinese medicine at the point of feishu,pishu for 5 hours or 10 hours after depilation.

治疗结束后观察支气管组织病理特征及细胞浸润情况，并对其进行气道炎症病理评分；酶联免疫吸附法测定被动吸烟大鼠肺泡灌洗液中α_1-抗胰蛋白酶、TNF-α、IL-8含量；黄嘌呤氧化酶法测定被动吸烟大鼠肺泡灌洗液SOD的活性、TBA法测定MDA的含量。

The activity of erythrocyte AR and 〓-ATPase, the level of the plasma NO and NOS, the content of sorbitol in sciatic and erythrocyte were detected by spectrophotography; The level of ET in plasma were detected by radioimmunassay .

荧光法测定红细胞AR活性；分光光度法测定红细胞膜〓-ATPase活性、血浆NO及NOS水平、坐骨神经及红细胞内山梨醇含量：放射免疫方法测定血浆ET水平：RT-PCR方法半定量坐骨神经内ARmRNA水平。

Groups were set as piglets immunized with pCIL2gD or pCIL6gD. Both antibody titres and CTL responses were tested and the results were compared with those of pcDD and inactivated vaccine groups.

用pCIL2gD和pCIL6gD分别免疫断奶仔猪，测定抗体水平、CTL活性及对病毒攻击的免疫保护，与pcDD免疫组的各项指标进行比较。

To elucidate the molecular basis of the attenuation ofDLA-EIAV in virulence thus provide theory foundation for designing HIV vaccine, thewhole genomes of DLA-EIAV and EJAV L provirus were cloned and sequenced. Aninfectious molecular clone derived from DLA-ELAV was constructed and characterized.Three DLA-EIAV/ L chimeric viruses were constructed. Promoting efficacy of the longterminal repeat of the DLA-EIAV was charactenzed.

为了揭示我国马传贫弱毒疫苗毒力致弱及免疫保护的分子机制，为人免疫缺陷病毒及其它慢病毒的免疫预防提供借鉴，我们对马传贫驴白细胞弱毒及其亲本马强毒EIAV L株前病毒进行了全基因克隆和序列测定，比较分析了二者的前病毒基因组核苷酸序列，在此基础上构建了EIAV驴白细胞弱毒株的感染性分子克隆和三株含有EIAV强/弱毒嵌合病毒基因组的重组质粒，并对EIAV弱毒株长末端重复序列的启动子活性进行了初步研究。

To elucidate the molecular basis of the attenuation ofDLA-EIAV in virulence thus provide theory foundation for designing HIV vaccine,thewhole genomes of DLA-EIAV and EIAV L provirus were cloned and sequenced.Aninfectious molecular clone derived from DLA-EIAV was constructed and characterized.Three DLA-EIAV/L chimeric viruses were constructed.Promoting efficacy of the longterminal repeatof the DLA-EIAV was characterized.

为了揭示我国马传贫弱毒疫苗毒力致弱及免疫保护的分子机制，为人免疫缺陷病毒及其它慢病毒的免疫预防提供借鉴，我们对马传贫驴白细胞弱毒及其亲本马强毒EIAV L株前病毒进行了全基因克隆和序列测定，比较分析了二者的前病毒基因组核苷酸序列，在此基础上构建了EIAV驴白细胞弱毒株的感染性分子克隆和三株含有EIAV强/弱毒嵌合病毒基因组的重组质粒，并对EIAV弱毒株长末端重复序列的启动子活性进行了初步研究。

Wistar rats were randomly divided into normal group, sham-operation group, 4 weeks diabetes mellitus model group (M4), 6 weeks diabetes mellitus model group (M6) and 8 weeks diabetes mellitus model group (M8). Behaviour was tested with Morris water maze and shuttle box test.

穿梭箱实验、Morris水迷宫实验检测大鼠认知行为学改变，免疫组织化学法、免疫印迹法、RT-PCR和酶联免疫吸附法检测BACE1；ELISA法检测Aβ在糖尿病大鼠脑组织中的表达，用图像分析仪测定吸光度值。

In our research,Wistar rats and SD rats which were parathyroidectomized were used as donors and recepients respectively.The parathyroid gland of the Wistar rat were allografted into the cerebroventricle and other different sites of the brains in SD rat s tereotaxically. Using methods of testing serum Ca~(2+) concentration,histological examination,histochemical and immunocytochemical methods and electron microscopic observation,we investigated the survival of the grafting tissue,its revascularization, infiltrating of monocytes, changes of microglia and astrocytes, the relationship of the CNS with the immune system, and the mechanism of rejection were investigated.The results as following: I .Effects on PTG after allografting into cerebroventricle and cerebral parenchyma of the brain: Testing serum Ca~(2+) conc.

本研究以Wistar大鼠作为供体，切除甲状旁腺的SD大鼠作为受体，在立体定位下将同种异体的PTG移植到大脑的不同部位，设计了侧脑室-内分泌系统移植的代替途径，比较了侧脑室内移植和脑实质内移植的异同，通过测定血清Ca~(2+)的浓度、组织学检查、组织化学和免疫细胞化学以及电镜观察对移植后PTG组织的存活、血管的再生、单核细胞的浸润、CNS内胶质细胞的变化、对免疫系统的影响及免疫排斥的机制进行了研究，结果如下：一、同种异体PTG侧脑室内移植与脑实质内移植的比较：血清Ca~(2+)的检查发现，PTG侧脑室内移植后第7天，血清Ca~(2+)的浓度恢复到8mg％以上，一直持续到移植后第8-12周；移植后第12周仍有60％移植PTG的动物血清Ca~(2+)维持在正常水平。

Pedal swelling induced by 1% agar in mice, dermal c apillary hyper-permeability induced by histamine in rats were used to evaluate the anti-inflammatory of Kangning granules, and the clearance rate of intraveno us ch arcoal particles was used to study its effect on non-specific immunologic funct i ons. Serum haemolysin level and the number of antibody secretory cells in spleen were determined to assess its effects of specific immunologic functions.

运用小鼠足跖肿胀和大鼠皮肤毛细血管通透性实验观察其对炎症反应的影响；用碳粒廓清实验评价其对非特异性免疫功能的影响、测定免疫小鼠血清抗体水平和脾细胞抗体分泌细胞数量以评价感冒康宁颗粒对特异性体液免疫功能的影响。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。