免疫抑制的

The results showed that LRK could significantly inhibit the mammary hyperplasia caused by estrin, reduce the content of serum 〓, raise P level, promote the production of DA in hypothalamus and mammary tissue, decrease the release of 5-HT, inhibit the secretion of serum PRL, reduce the sensitivity of mammary tissue to estrin and progestogen, prevent the expression of mammary tissue on PCNA, the effect was equal to triphenylamine oxide, obviously superior to the control group of Ru Zeng Ning.

通过实验研究，建立了具有肾虚肝郁证侯群的乳腺增生病理模型，利用免疫组化技术、图像分析系统、荧光分光光度法、放射免疫法等现代医学研究方法，从病理形态学、医用体视学、神经内分泌学、阴道细胞学等方面，观察了利乳康口服液对模型动物整体状态、乳腺组织病理形态、血清激素、神经递质以及卵巢功能的影响，结果表明利乳康口服液能明显抑制雌激素引起的乳腺组织增生，降低血清E〓含量，升高P水平，促进下丘脑及乳腺组织中DA的产生，减少5-HT释放，抑制血清PRL分泌，降低乳腺组织对雌、孕激素的敏感性，阻止乳腺组织PCNA的表达，其效应与三苯氧胺相当，明显优于乳增宁对照组；另外，利乳康口服液还能缓解模型动物肾虚肝郁证侯群，增加其体重，调节其整体状况，改善模型动物不规则动情周期，减轻其子宫及卵巢的病变，抑制子宫内膜的过度增长，调节卵巢功能，揭示了中药利乳康口服液对神经内分泌和卵巢功能的整体调控作用，以及对本病发生、发展的预防作用

It is characterized by malignant unusual hyperplasia and soading of cells in medulla ossium and other tissues, which can damage the normal hemopoiesis function and the mechanism of blood coagulation fibrinolysis and immunology.

机体的免疫功能与肿瘤的发生和发展有密切关系，当宿主免疫功能与肿瘤的发生和发展有密切关系，当宿主免疫功能低下或受抑制时，肿瘤发病率增高，而在肿瘤进行性生长时，患者的免疫功能进一步受到抑制。

A series of indexes reflecting free radical changes, anti-oxidations, Immune function, endocrine function was studied by Chemiluminescence method, immunoturbidimetry, ANAE dye method, micro-hemagglutination inhibition test and radio immunoassay in the chicken with encephalomalicia induced by giving the feed being deficient in VE and extra fish oil artificially. The pathological changes including microscopic and ultramicroscopic changes in the relative organs and tissues were observed.

本实验以一日龄150只海兰兰蛋鸡为实验动物，用低维生素E且添加鱼油日粮饲喂雏鸡，复制脑软化症动物模型，采用化学发光法、免疫浊度测定法、酸性α-醋酸萘脂酶染色法、微量血凝抑制试验、放射免疫测定技术等方法系统地检测了脑软化雏鸡的自由基代谢和抗氧化功能、红细胞免疫功能、细胞免疫功能、体液免疫功能及非特异性免疫功能等指标，并对患雏病理组织进行了显微及超微结构观察。

Through active immunization of Inhibin antige n from porcine seminal plasma and super ovulation of allochthonous purified FSH, the effect of IB active immunization on cow was studied and its mechanism was al so further ascertained by examination of relative hormone concentrations in seru m at different physiological periold.12 Hostein cows and 12 Xiza young cows of 3 -5 years old (postpartum 50 d and above),were selected and divided into the exp e rimental group and control group randomly according to the estrum order.

张志胜 ，田树军，王志刚，桑润滋用猪精液抑制素抗原主动免疫供体母牛，结合外源纯化FSH超排处理，观察了抑制素主动免疫对牛超排效果的影响，并通过检测不同生理时期血液中相关生殖激素的水平，探讨了抑制素免疫影响牛超排效果的机制。

Thunberg Fritillary Bulb is one of the most popular Traditional Chinese herbs. Some trials in vivo and animals indicated that Peimine and Peiminine, extracted from Thunbery Fritillary Bulb, have the function of reversing MDR of two different kinds of mechanisms. They can improve the cytotoxic of ADR to cancer cells by 5～10 folds under concentration of 10% cancer cell growth inhibitory rate. Peimine and Peiminine not only feather typical character of modifier of MDR, but also differ from other moldifiers such as Calcium channel blocker, Cyclosporines in chemical structure, they belong to cevine groups alkaloid, a subgroup of C-Nor-D-homosteroid alkaloid.

浙贝母是临床常用中药，体外及动物试验证明，浙贝母中的有效活性成分贝母甲素和贝母乙素具有逆转肿瘤细胞MDR的作用，而且能逆转两种不同机制的多药耐药肿瘤细胞的耐药性，在无明显细胞毒剂量下，可使阿霉素对耐药肿瘤细胞的杀伤活性提高5-10倍，不但具备明确的多药耐药逆转剂的特征，而且在化学结构上属异甾类生物碱中的瑟文类生物碱，完全不同于钙离子拮抗剂、免疫抑制剂和其它现有的耐药逆转剂，具有作用靶点宽、化学结构独特的特点。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

IDO inhibits the proliferation of eukaryotic intracellular pathogens or tumor cells by depriving them of tryptophan. IDO has been targeted for neurological disorders: it causes decrease of serotonin level in the brain resulting depression, and it causes the accumulation of neurotoxic quinolinic acid. IDO plays a key role in the suppression of T cell response and antitumor immune, it induces immune tolerance to embryo and explant.

IDO可通过降低微环境中色氨酸的浓度而达到抑制病原微生物增殖的作用； IDO与神经系统疾病也密切相关，它能降低5-羟色胺的水平而导致抑郁，也可造成脑中喹啉酸等具有神经毒性的代谢产物的累积； IDO在抑制T细胞免疫和抗肿瘤免疫、诱导母胎免疫耐受和移植物免疫耐受中均发挥重要的代谢性免疫调节作用。

These results indiacated that tranferring macrophages combinedwith cytokines could be a new adoptive immunotherapy protocol foradvanced cancer,and rTNF used in vivo locally could induce andactivate macrophages to kill tumor cells.Monocytes when activatedunderwent a series of phenotypic and functional changes includingthe expression of IL-2R which may provide an important immunoregu-latory pathway.The presence of lectin-like molecules on thesurface of monocytes and tumor cells may bring theeffector/target cells together,thus facilitating the inductionof apoptosis in target cells by triggering the production ofcytolytic factors and the modification of target of targer cell surface anti-gens.(such as HLA-DR).

综合以上结果可以得出以下结论：转输巨噬细胞并结合细胞因子的联合应用是肿瘤继承性免疫中的又一新的、有潜力的方法；抗肿瘤细胞因子rTNF可通过激活巨噬细胞在体内抑制对其不敏感的肿瘤细胞的生长；人单核细胞在一定条件下激活后，可表达IL-2R，进而一方面增强其自身对IL-2的敏感性，另一方面在体内也具有调节T细胞功能的作用；单核细胞和肿瘤细胞上存在的凝集素类受体可促使两种细胞之间在凝集素介导下相互接近，并诱发单核细胞产生细胞毒因子，以及通过调节靶细胞上表面抗原的表达，促进诱导靶细胞的细胞凋亡。

Allogeneic and syngeneic fresh bone, autolyzed antigen-extracted bone, bone matrix gelatin, demineralized bone matrix were implanted into the muscle pouch of mice, at different times after implantation, the immunological, histological and alkaline phosphatase assay were conducted. The results revealed that all four kinds of allogeneic implants activated specific cellular and humoral immune responses, most notably in fresh bone group, the AAA, BMG and DBM inhibited the proliferation of the lymphocytes in vitro and BMG had the most powerful inhibiting action, allogeneic AAA, BMG and DBM might induce heterotopic osteogenesis in vivo, however, there were obvious difference in ALP and histomorphometry between allografts and isografts.

在小鼠肌肉内植入同种异体或同系新鲜骨、自消化抗原去除骨、骨基质明胶、脱钙骨基质，术后免疫学、碱性磷酸酶、组织学检查发现：4种同种异体植入物均可引发特异性细胞免疫反应和体液免疫反应，其中以新鲜骨移植引发的免疫反应最为显著；自消化抗原去除骨、骨基质明胶、脱钙骨基质在体外具有抑制淋巴细胞增殖的作用，骨基质明胶对淋巴细胞增殖的抑制作用最显著；同种异体与同系4种植入物在体内成骨活性有显著差异；自消化抗原去除骨、骨基质明胶、脱钙骨基质均有异位诱导成骨活性，其中骨基质明胶骨诱导活性最佳。

objective to compare image manifestations values between high resolution computerized tomography and x-ray chest film in idiopathic interstitial pneumonia in the elderly.methods we analysed and compared image manifestations between hrct and x-ray chest film with 12 cases of clinically suspected iip in the elderly.results ct and hrct had more sensitivity and specificity than x-ray chest film.there were more image manifestations in hrct such as grand grass,net,line,nodular,honeycombing,bronchiectasis,honeycombing cyst,peribronchovascular interstitial thickening and irregularity.there were significant difference between them （p.05）.these patients who were suspected iip had better control and remission after being treated with hormone and immuno-suppressive drug.conclusion it have more significant virtues in hrct to manifest iip in the elderly than x-ray chest film,and it also may manifest iip curative effect.

目的 比较高分辨断层摄影术、x线胸片检查对老年特发性间质性肺炎的诊断价值。方法对12例临床疑诊为间质性肺炎的老年患者，进行胸部hrct与胸部平片检查，并对照分析。结果胸部ct在诊断方面比常规胸片有较大的优越性，hrct则有更高的敏感性和特异性，hrct表现出磨玻璃影、网状、线条、小结节、蜂窝影，支气管扩张、细支气管扩张、支气管壁和血管壁增厚及不规则等征象，12例临床疑诊为间质性肺炎的患者，经胸部hrct诊断的12例，而胸部平片诊断的仅有2例，二者比较差异有显著性（p.05），hrct疑诊的iip患者，经临床激素及免疫抑制剂治疗，病情均得到控制或缓解。结论 hrct表现老年间质性肺炎的特点明显高于胸部平片，且hrct可以反映间质性肺炎的疗效。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。