免疫原性

Recombinant colony, E.coli DH5α(pMG320),expressing O\|antigen of V.cholerae O 139 was detected from the bank by immunological agglutinative reaction.

经鉴定分析重组克隆所表达的O 抗原具有良好的免疫原性及反应原性。酶切分析质粒 pMG32 0 ，推知其O 抗原基因大小约 4 6kb。

Results HlyA436 was successfully expressed in prokaryotic expression system, have elicited high titer antiserum and has certain protective efficacy.

结果 HlyAN436成功地在大肠杆菌表达系统表达，动物实验证实其具有良好的免疫原性和反应性以及一定的免疫保护性。

Evaluation of IEM103 in rabbit animal model.The evaluation was based on the control-challenge study (IEM101 as control). Rabbits were laparotomized, injecteded 10〓 cfu directly into proximal small bowel, and 28 days later, challenged with 0.25--2ug CT and 10〓-10〓 cfu 4 wild toxic strains with different biotypes and serotypes. Experimental group developed antitoxic and significant vibriocidal immune responses to vaccination, and could protect no less than 2ug CT and 10〓-10〓 cfu all wild strains challenge.

IEM103的免疫原性评价：经兔肠段结扎免疫〓cfu IEM103，7天后在外周血中可检测到外周淋巴细胞分泌性抗CT IgG，血清中可检测到抗CT抗体、杀弧菌抗体，P／N均≥2，且可持续至28天。28天后用0.25-2ug CT毒素，四种毒株滨-43，吴江-2，395，1119〓cfu菌量攻毒，实验组可保护至少2ug CT攻毒，而对照组可保护1ug以下CT攻毒，两组均可保护〓cfu四种毒株攻毒（以肠积液量≥1.0ml／cm为不保护判断标准）。

The homology of the nucleotide sequences and genetic variation analysis of NDV strains suggested that mere were obvious divergence in gene lever among NDV vaccine strains and epizootic strains isolated from different areas and different ages.The divergence may have effect on amino acid and structure and function of protein in some extent and lead to difference of immunogenicity ,which was one of reason being difficult in preventing from ND.

通过对NDV F基因核苷酸序列同源性比较及遗传变异分析发现：我国不同年代、不同地区分离的NDV毒株与疫苗毒株之间在基因水平上存在较大差异，这种差异对氨基酸及蛋白质的结构和功能可能会有一定影响，而NDVF蛋白的差异同时也造成免疫原性的差异，这很可能是新城疫的免疫防治十分困难的原因之一。

These results suggest that hemolysin is enterotoxigenic.Other experiments confirmed that hemolysin had immunity and immunoreaction.

提示蜡样芽胞杆菌溶血素具有肠毒素的性质，试验也证实所提取的溶血素有按摩免疫原性和按摩免疫反应性

Be a kind of big member of glycoproteins, it is still a kind of immunogen, and it can be divided into various serotypes according to the immunogen of its heavy chain constant region.

作为一种大分子糖蛋白，Ig 还是一种强的免疫原，并可根据其重链恒定区的免疫原性差异将其分为多种血清型。

To evaluate the diversity of immunogenicity of IROMPs, OMPs and whole-cell bacterin of leporid P. multocida, and evaluate their cross protection in mouse model.

本试验以小鼠为动物模型，比较了兔多杀性巴氏杆菌铁调节外膜蛋白的免疫原性及分析交叉免疫保护效果。

Viral vector mainly contain adenovirus, retrovirus,adeno-associated virus and so on, but it has potential danger of safety; it isrepeled by immune system when it is injected to organism for a greatimmunogenicity. An injection with adenovirus vector with highconcentration, it leads to serious inflammatory reaction of liver. Viralvector such as liposome and polycation are commonly used lately. But,liposome and polycation have low specificness and targetness of genetransfer tissue, have lower transfection efficiency and short period ofgene expression, for they can be phagocytized by endothelial system.

许多的载体，病毒载体和非病毒载体以前已广泛应用，病毒载体主要包括腺病毒，逆转录病毒，腺相关病毒等；但病毒载体在安全性方面存在潜在的危险；免疫原性比较强，注射到机体后很快会被机体的免疫系统排斥，当静脉注射高浓度的腺病毒载体会使肝脏发生严重的炎症反应；非病毒载体目前常用的有脂质体及多聚阳离子聚合物；但脂质体和阳离子聚合物介导基因转移缺乏组织的特异性和靶向性，转染效率较低且易被网状内皮系统吞噬，基因表达时间短；因此研制新型的非病毒载体已成为研究的热点，纳米颗粒具有小尺寸效应，表面效应，随着颗粒直径变小，比表面积将会显著增大，故具有很高的化学活性，因而纳米成为了最有应用前景的非病毒载体。

This suggested that the purified and dialyzed SjR47 fractional protein should have better immunogenicity and immunoreactivity, and share egg origin antigen.

实验结果表明，经纯化透析后的SjR47组分蛋白具有较高的免疫原性和免疫反应性，该蛋白分子属血吸虫卵源性组分抗原。

It is suggested that six OMPs ofV.parahaemolyticus ompV, ompW, ompK, ompU, psuA and pvuA, are immunogenicduring in vivo infection, and may be of great significance for vaccine development.Especially, fish group immunized with recombinant ompK achieved a high protection of90%. For the wide expression of this conserved protein among vibrio species, ompKmust be paid enough attention for vaccine development against vibriosis.

研究结果显示了6种副溶血弧菌外膜蛋白ompW、ompV、ompK、ompU、psuA和pvuA具有良好的免疫原性，是宿主免疫应答的保护性抗原；尤其是ompK单个蛋白免疫组获得90％的保护率，由于该蛋白是弧菌中广泛存在的保守性蛋白，极有可能作为制备亚单位疫苗等高效弧菌疫苗的候选抗原。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。