免疫原性

The effect of the specific inhibitor cibacron blue of GSTs and antiparasitic drugs albendazole, praziquantel and artesunate on the activities of recombinant GSTM, GST1 and GST2 was evaluated.

研究目的解析三个胞质GSTs的基因结构及其特点，克隆三个胞质GSTs基因，鉴定原核表达的重组GSTs的免疫反应性、免疫原性、催化活性及其免疫定位和抑制剂、常用抗寄生虫药对催化活性的影响。

Coli mainly in a form of inclusion body. The expressed product contained about 25% of total somatic protein and showed good reactogenicity as proved by Western blot. The IgG level against GAMP induced by GAMP-CRM197 conjugate was significantly higher than those by GAMP and by the mixture of CAMP and rCRM197. Repeated immunization with the conjugate induced immunopotentiation, indicating the effect of CRM197 as a carrier protein.

结果重组CRM197在大肠杆菌中主要以包涵体形式表达，表达量占菌体总蛋白的25%左右；Western blot证明重组rCRM197具有良好的反应原性；各针接种后，结合物诱生的多糖特异性IgG水平均显著高于GAMP组和GAMP+rCRM197混合物组，具有较强的免疫原性；多次接种产生了免疫增强效应，重组CRM197具有载体蛋白的作用。

The present invention relates to immunogenic compositions for intradermal delivery of an antigenic or immunogenic agent in combination with one or more excipients.

本发明涉及免疫原性组合物，其用于皮内输送与一种或多种赋形剂相组合的抗原性或免疫原性试剂。

Based on these previous work, in this research, in order to explore the the molecular mechanism of enhanced tumor cell immunogenicity treated by elemene and mytomycin C compositely, we used murine Hca-F ascitic hepatic carcinoma ( high lymphatic metastasis substrain of H22) as an experimental model to compare the immunoprotective effects on Hca-F cells as well as the mechanism of the effects caused by HSP70-peptide complex purified from untreated tumor cells, EC-TCV and BCG; We studied the mechanism of increased tumor cell membrane HSP expression treated with elemene by the techniques of RT-PCR and immunoelectron microscopy; In order to deeply elucidate the antitumor effects and the molecular mechanism of elemene in increasing tumor cell immunogenicity we analyzed and compared the effects of elemene and heat shock on the gene expressions of tumor cell using gene expression profile chip.

本论文在上述研究成果的基础上，选择小鼠肝癌H22的高淋巴道转移亚系Hca-F为模型，进一步比较了未处理肿瘤细胞来源、榄香烯复合瘤苗来源的和卡介苗来源的HSP70-肽复合物对Hca-F的免疫保护效应、效应机制，探讨榄香烯-丝裂霉素复合处理增强肿瘤细胞免疫原性的分子机制；以Hca-F和人肝癌细胞株HepG〓等为模型研究了榄香烯处理增强肿瘤细胞膜热休克蛋白表达的机制；以HepG〓为模型用表达谱基因芯片研究比较了榄香烯与热休克处理对肿瘤细胞基因表达的影响，从而进一步阐明榄香烯抗肿瘤作用及增强肿瘤细胞免疫原性的分子机制。

Healthy rabbits were inoculated each with vaccines of the recombinant Pilin and the recombinant fused Pilinn-IL2 protein at 250ug per dose to potentiate the immunogenicity. The D. nodosus Pilin vaccine and the killed D. nodosus whole cell vaccine were also inoculated into rabbits as controls in order to compare with the above two recombinant vaccines.

用纤毛蛋白基因工程疫苗、Pilin-IL-2融合基因工程疫苗分别免疫健康家兔，初步进行了纤毛蛋白疫苗和Pilin-IL-2融合基因工程疫苗的免疫原性试验，并同时用节瘤拟杆菌菌体灭活疫苗及菌体纤毛蛋白疫苗免疫家兔，进行了免疫原性比较试验。

Apoptotic cells have immunogenicity,and may be the major source of autoantigens in lupus BXSB mice.Lupus genetic factors and Yaa gene can potentiate the immunogenicity of apoptotic cells or accelerate their inducing the lupus nephritis.

凋亡细胞具有免疫原性，它可能是狼疮性BXSB小鼠体内自身抗原的主要来源；狼疮性遗传因素和Yaa基因能促进凋亡细胞的免疫原性和增强凋亡细胞的致肾炎作用。

Apoptotic cells have immunogenicity,and may be the major source of autoantigens in lupus BXSB mice.Lupus gecnic factors and Yaa gene can potentiate the immunogenicity of apoptotic cells or accelerate their inducing the lupus nephritis.

凋亡细胞具有免疫原性，它可能是狼疮性BXSB小鼠体内自身抗原的主要来源；狼疮性遗传因素和Yaa基因能促进凋亡细胞的免疫原性和增强凋亡细胞的致肾炎作用。

ConclusionApoptotic cells have immunogenicity,and may be the major source of autoantigens in lupus BXSB mice.Lupus genetic factors and Yaa gene can potentiate the immunogenicity of apoptotic cells or accelerate their inducing the lupus nephritis.

结论凋亡细胞具有免疫原性，它可能是狼疮性BXSB小鼠体内自身抗原的主要来源；狼疮性遗传因素和Yaa基因能促进凋亡细胞的免疫原性和增强凋亡细胞的致肾炎作用。

Apoptotic cells have immunogenicity,and may be the major source of autoantigens in lupus BXSB mice.Lupus genetic factors and Yaa gene can potentiate the immunogenicity of apoptotic cells or accelerate their inducing the lupus nephritis. Apoptosis; Mice,BXSB; Lupus erythematosus,systemic

凋亡细胞具有免疫原性，它可能是狼疮性BXSB小鼠体内自身抗原的主要来源；狼疮性遗传因素和Yaa基因能促进凋亡细胞的免疫原性和增强凋亡细胞的致肾炎作用。

Rabbit was immunized by the conjugated alginate-BSA (1.0mg/kg) for 40-days routine immunity method; ELISA method was used to examine the titration of anti-alginate serum; The first generation parotid acinar cells were chosen to divided five groups (group A :contrast, group B: BAS, group C: alginate, group D: anti-alginate serum, group E: alginate +anti-alginate serum), We examined the proliferation of each part by MTT method at four times (lh, 6h,12h and 24h); In the meanwhile, The growth and shape of parotid acinar cells were observed under phase contrast microscope and scanning electron microscope.16 of the immunized rabbits by alginate were divided equally into Al and Bl groups, 12 normal rabbits were divided equally into A2 and B2 groups as control groups, Each rabbit was affused by 0.3ml(40fil/ml)alginate though parotid duct once time two days.

将藻酸盐与BSA偶连形成具有较强免疫原性的Alginate-BSA交联物免疫兔，按抗原量1.0mg/kg接种于新西兰白兔皮内，间隔10d加强免疫1次，共加强4次后，利用间接ELISA法检测藻酸盐抗体效价：将原代培养的兔腮腺腺细胞按10~4个/ml接种到96孔板上，置入细胞培养箱继续培养3d，分为5组：A空白对照组、B牛血清白蛋白组、C藻酸盐组、D抗藻酸盐血清组和E抗藻酸盐血清+藻酸盐组，分别加入PBS、牛血清白蛋白、藻酸盐或新鲜抗藻酸盐血清继续培养，分别于1h、6h、12h和24h不同的时间点，取出96孔培养板，采用MTT法检测5组腮腺腺细胞增殖情况

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。