免疫单位

Weeks later, all the rats were killed, the levels of sodium pump α1-,α2-, and α3-subunit mRNA and protein in the cortex of kidney were detected with reverse transcription polymerase chain reaction method and immunohistochemical assay, respectively.

分别应用RT-PCR及免疫组化技术，探讨1k1c高血压大鼠肾脏皮质钠泵α1、α2及α3亚单位mRNA及蛋白水平基因表达的改变。

Weeks later, all the rats were killed and sodium pump α1-,α2-, and α3-subunit in aortic smooth muscles were detected with reverse transcription p ol ymerase chain reaction method and immunohistochemical assay at both mR NA and protein levels, respectively.

分别应用分子生物学 RT-PCR及免疫组化技术，探讨1k1c高血压大鼠动脉平滑肌细胞钠泵α1、α2及α3亚单位mR NA及蛋白水平基因表达的改变。

To get the censored units by two?stage PPS sampling,investigate its service forms and service periods of EPI,together with basic vaccination rate.Quality control measures were managed through investigations. Software SAS 6.12 was applied for analysis of canonical correlation and redundancy.

按两阶段PPS法抽取审评单位，调查预防接种服务形式、服务周期和基础免疫接种率，在整个调查过程中予以质量控制，并应用SAS6.12进行典型相关分析和冗余度分析。

Methods: Single lung in situ ischemic reperfusion animal model was used. 30 rabbits were randomly divided into three groups: sham group, ischemic/reperfusion group and ischemic preconditioning group. Wet to dry ratio and injured alveoli rate were determined. Ultramicroscopic structural change was observed under electron microscope. Heat shock protein-27 was detected with immunohistochemistry.

采用在体兔单位缺血瑞灌注模型，30只日本大耳兔随机均分为三组：假手术组、缺血／再灌注组及缺血预处理组，实验检测肺组织混干重比和肺泡损伤数比值，电镜评定肺组织起微结构损伤，免疫组化检测肺组织热休克蛋白27的定位及表达。

The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

Non-fluorescent double staining technique was used to retrogradely identify the neurons immunocytochemically.

采用霍乱毒素B亚单位结合胶体金逆行标认神经元复合免疫细胞化学反应的非荧光双标方法。

Non-fluorescent double staining technique was used to retrogradely identify the neurons immunocytochemically.

采用霍乱毒素B亚单位结合胶体金逆行标认成年人神经元复合免疫孕妇细胞早泻化学反应的非荧光双标方法。

Results The rubrospinal neurons were retrogradely identified by cholera toxin subuint B coulpled with colloidal gold (CB-Au,prepared by our laboratory).

采用霍乱毒素B亚单位结合胶体金逆行标认神经元复合免疫细胞化学反应的非荧光双标方法。

Dryads - 145 Gold for Slow Poison, Superfast speed, Magic Immune, High-damage ranged units.

森林女神—只值145金的慢性毒药、超快速度、魔法免疫加高伤害远程单位，只要145金！

Aim:(1) The purpose of this study is to examine whether protein tyrosine kinase and AMPA receptor in the nucleus tractus solitarius and nucleus ambiguus were involved in the regulation of normoxia and hypoxia respiratory response by microinjection of PTK inhibitor and non-NMDA receptor antagonist;(2) To observe the change of number of phosphotyrosine immunopositive neurons in NTS and NA before and after hypoxia using immunohistochemical methods and whether tyrosine phosphorylation and GluR1 in postsynaptic membrane co-localize in NTS and NA neurons.

目的：(1)观察向家兔脑干孤束核或疑核内微量注射酪氨酸蛋白激酶阻断剂和谷氨酸AMPA受体阻断剂对常氧呼吸和低氧呼吸反应的影响，探讨PTK和AMPA受体在低氧呼吸反应中的作用。(2)观察低氧通气后NTS和NA磷酸化酪氨酸免疫阳性神经元的数量变化；观察磷酸化酪氨酸和AMPA受体亚单位GluR1在突触后膜共存的情况，推测在低氧呼吸反应中AMPA受体和PTK信号转导途径的关系。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。