免疫单位

Immunofluorescence confirmed that sweat glands in normal mice did not present gamma-aminobutyric acid receptor subtype GABAA andα-amino-3-hydroxy-5-methylisoxazole-4-propionic acid/kainic acid subtype of glutamate receptor. Mature mice with reactive sweat glands that declined more than 25% compared to baseline were defined as anhidrotic mice.

免疫组织荧光染色未发现在正常小鼠皮肤组织及汗腺的分泌细胞存在γ-氨基丁酸A型(gamma-aminobutyric acid，GABAA)受体α1亚单位、谷氨酸受体亚型α-氨基羟甲基恶唑丙酸(α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid ， AMPA )GluR1/2/3/4亚单位和海人酸(kainic acid，KA)受体GluR5/6/7亚单位的表达。

Immunofluorescence confirmed that sweat glands in normal mice did not present gamma-aminobutyric acid receptor subtype GABAA andα-amino-3-hydroxy-5-methylisoxazole-4-propionic acid/kainic acid subtype of glutamate receptor.Mature mice with reactive sweat glands that declined more than 25% compared to baseline were defined as anhidrotic mice.

免疫组织荧光染色未发现在正常小鼠皮肤组织及汗腺的分泌细胞存在γ-氨基丁酸A型(gamma-aminobutyric acid，GABAA)受体α1亚单位、谷氨酸受体亚型α-氨基羟甲基恶唑丙酸α-amino(来源：Ad1d1BC论文网www.abclunwen.com-3-hydroxy-5-methylisoxazole-4-propionic acid ， AMPA GluR1/2/3/4亚单位和海人酸(kainic acid，KA)受体GluR5/6/7亚单位的表达。

The heterologous protein fragments are so-called CTL epitope, which is the linear fragment or the space conformation structure recognized specifically by immune cell and plays a key role in CTLs activation. It is the elementary event of inducing immune responses and it decides CTL specific cytotoxic effects, therefore, selection of antigen epitope makes the pivotal step to identification of specific antibodies.

抗原肽是抗原中能被免疫细胞特异性识别的线性片段或空间构象性结构，是引起免疫应答和免疫反应的基本单位，在机体免疫中发挥关键的作用，抗原表位的选择也是能否获得特异性抗体的关键。

Ensnare - This ability is no longer dispellable, and it can target magic-immune units.

诱捕 -这个技能不再能够被驱散，并且可以作用于魔法免疫单位。

Increased Lumber Mill's cost to 375, buffed Shredder's armor, hitpoints, attackspeed, cleave range and added an explode on death ability and an inducted rage ability

增加伐木场花费为375，强化伐木机的护甲，血量，攻速，攻击距离，增加死亡爆炸和激怒能力增加阻挡特效，当免疫单位被特殊塔集中

Through immunocytochemistry study, we found that the α3 and/or α5 subunits of nicotinic acetylcholine receptor are predominantly expressed in the oxytocinergic neurons. Our finding is quite different from the conclusion of the British scholar Dr. Mason's work in 1980s. We also found in this study that, the α3 and/or α5 subunits of N-AChR can also be expressed on the axons of oxytocinergic neurons, and these immunoreactive axons could be seen projecting to the median eminence and further to the posterior end of neurohypophysis. In the hypothalamus of the 7-day postnatal pup rats, the expression patterns of α3 and/or α5 subunits of N-AChR are similar to that in adult rats.

我们用免疫组化的方法发现，α3和/或α5型烟碱型乙酰胆碱受体(Nicotinic acetylcholine receptor，N-AChR)亚单位在大鼠下丘脑OT能神经元中呈优势性的表达，这与上世纪八十年代英国学者Mason类似工作的结论不一致；而且我们还发现α3和/或α5型N-AChR亚单位还表达在OT能神经元的轴突，此阳性免疫反应的轴突一直延续到正中隆起和神经垂体的末端；在新生7天大鼠的下丘脑，α3和/或α5型N-AChR亚单位的分布类似成年鼠。

Serum immunoglobulin of Amur sturgeon Acipenser schrenckii, Chinese sturgeon Acipenser sinesis and Huso sturgeon Huso dauricus which were healthy and unimmuned, was first time respectively purified by using saturation ammonium sulfate precipitation followed by Sephadex G-200 gel column chromatography. Molecular weight and partial characterization of Ig and heavy chain and light chain of Ig were measured by polyacrylamide gel electrophoresis, SDS polyacrylamide gel electrophoresis, Western blotting and Ouchterlony method.

采用饱和硫酸氨分步沉淀和Sephadex G-200凝胶层析的方法，首次分别纯化制备了健康非免疫状态下中华鲟、史氏鲟和达氏鳇的血清免疫球蛋白，并采用聚丙烯酰胺凝胶电泳、SDS-聚丙烯酰胺凝胶电泳、蛋白免疫印迹及免疫琼扩实验等方法对其Ig及Ig亚单位的分子量和部分特性进行了分析。

Methods The SpaA was purified by electroelution from NaOH-extracted antigen of Erysipelothrix rhusiopathiae strain C43311. The rSpaA-N was expressed in E. coli BL21 as a soluble protein by IPTG inducing,and purified with GST affinity chromatography. Mice of each group were subcutaneously immunized three times with 50μg or 100 μg of native SpaA, rSpaA-N or NaOH-extracted antigen with in complete or incomplete Freund adjuvant at 2-week intervals.

将猪丹毒丝菌C43311株SpaA-N以可溶形式表达在大肠杆菌BL21中，用GST Bind Resin纯化试剂盒纯化rSpaA-N，采用电洗脱法从猪丹毒丝菌C43311株NaOH提取抗原中纯化天然SpaA，将rSpaA-N、天然SpaA和NaOH提取抗原制成亚单位疫苗，同时设GST及生理盐水对照组，间隔2周分3次皮下免疫小鼠，第3次免疫后2周用100LD50猪丹毒丝菌C43065株进行腹腔攻毒，采用间接ELISA方法检测免疫组小鼠血清的抗体动态变化。

The dynamic changes of the number of T or B lymphocytes and the content of immunoglobulin G, IgM, IgA in peripheral blood, the number of CD〓, CD〓 lymphocyte subpopulation, T〓 cells and IgG, IgM, IgA antibody-producing cells, the ratios of CD〓 and CD〓 T cells in immune organs (thymus, bursa of Fabricius and spleen) and immune tissues (cecal tonsils and Harderian glands), the content of IgA, IgM, IgG in local fluid (tear, tracheal fluid, intestinal fluid and bile) of one-day chicks infected with chicken infectious anemia virus and CIAV-infected chicks immunized with Newcastle disease vaccine were determined respectively by immunohistochemistry, immunogold-silves and immunoenzyme staining in order to reveal mechanism of immunopathogenesis of chicks infected with CIAV and influence of immune responses of CIAV-infected chicks to ND vaccine.

中文题名雏鸡感染鸡传染性贫血病毒后免疫变化和对新城疫疫苗的应答及其免疫机理副题名外文题名 Immune changes of chicks infected with chicken infectious anemia virus and immune response and mechanism to Newcastle disease vaccine immunity 论文作者郑世民导师刘忠贵教授学科专业基础兽医学研究领域\研究方向学位级别博士学位授予单位东北农业大学学位授予日期2002 论文页码总数105页关键词鸡传染性贫血病雏鸡新城疫疫苗馆藏号BSLW /2003 /S858 /9 本项研究采用免疫组织化学、免疫胶体金及免疫酶方法，首次对CIAV感染1日龄雏鸡和CIAV感染雏鸡ND疫苗免疫及vNDV攻击后，其外周血液的T细胞、B细胞数量和IgG、IgM、IgA含量；中枢免疫器官胸腺和法氏囊，外周免疫器官脾脏及呼吸道和消化道局部粘膜免疫组织哈德尔腺和盲肠扁桃体的ANAE〓、CD4〓、CD〓T细胞、IgG、IgM、IgA抗体生成细胞数量和CD〓与CD〓T细胞比值以及泪液、气管液、肠液、胆汁的IgA、IgM、IgG含量的动态变化规律和CIAV感染对ND疫苗的免疫保护率等进行了全面系统地研究。

Fluorescence resonance energy transfer analysis of subunit assembly of the ASIC channel Homo-and heteromeric ASIC complexes have been verified by classical methods such as co-immunoprecipitation,or functional assays applying electrophysiology.However,the exact subunit assembly and stoichiometry of heteromeric ASIC channels in living cells are largely unknown.

用荧光共振能量转移方法研究酸敏感离子通道的装配用经典的方法如免疫共沉淀或电生理分析，认为大多数亚单位可以形成同聚体或异聚体复合物，然而关于活细胞中ASICs亚单位装配以及ASICs异聚体中各亚单位分子比的情况并不清楚。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。