免疫化学

The distribution of the growth-associated protein (GAP-43) which is close related to the axon regeneration was examined by immunocytochemical method in the adult rat brain and the rat parietal lobus injuried into which the fetal cerebral suspension was lransplanted.

在发现流式细胞计可用于神经科学的研究后，用FCM研究了大鼠脑发育过程中细胞周期的变化和胚胎大脑细胞移植到成年大鼠顶叶后对宿主顶叶脑细胞分裂的影响；由于生长相关蛋白(GAP-43)与轴突再生密切相关，用免疫细胞化学法研究了GAP-43在成年大鼠脑内的分布及顶叶损伤植入胚胎大脑细胞后GAP-43的变化。

Methods 5-HT immunoreaction in rats brain was detected after the acute and chronic adminisstration of four antidepressant drugs by immunohistochemistry methos.

采用免疫组织化学方法，对一次和长期给予4种抗抑郁药的大鼠进行研究。

Results After 2 weeks, the number of skeletal myoblasts reached 107 per millilitre. Immunocytochemical analysis showed that over 90% cells were stained positive for myogenin, a factor specific to skeletal myocytes.

结果 采用组织块培养法，2周后成肌细胞的密度可达107/ml，免疫细胞化学分析显示90%以上的细胞呈骨骼肌特异的myogenin抗体染色阳性。

And each group included 10 cases as well as 10 cases of normal third molars teeth as control.

采用免疫组织化学方法观察MMP-8在不同龋损中表达。

In this reach ,we detected the change of nucleus of apoptotic cell and quantity of apoptotic bodies by the technique of immunofluorescence,and the actitity of the en- zymes concerned with cell apoptosis was detected by chemical methods.

本研究应用免疫荧光技术检测凋亡细胞核变化和凋亡小体的数量，同时还应用化学方法检测与细胞凋亡有关酶的活性。

Results 24 patients of melanoma in primary female genital tract,13 cases occurred in valvar,7 vagina,4 cervical.

复习临床、病理学资料，全部病例进行随访，免疫组织化学方法用s|p法。

Immunohistochemical analysis for CD3, CD4, CD8, CD20, CD56, Fas and FasLwas performed, and the types and distribution of TILs in the three kinds of tumorswas measured.

采用免疫组织化学染色，检测三种肿瘤组织中CD3、CD4、CD8、CD20和CD56的表达，分析三种肿瘤中TILs的种类和分布特点；并检测三种肿瘤组织中Fas和FasL的表达； 3。

Methods: Proximal tibias from neonatal rats were collected. The protein expressions of VEGF and its receptors Flt-1 and KDR/Flk-1 in osteoblasts in proximal tibias of neonatal rat were detected by immunohistochemistry.

切取新生鼠胫骨上端组织，采用免疫组织化学染色方法，检测VEGF及其受体Flt -1和KDR/Flk -1在新生鼠胫骨上端成骨细胞中蛋白质的表达。

METHODS: Lipofectamine method: The BMMSCs were obtained from the tibias and femurs of the guinea pigs. The third passage BMMSCs were cultured with plasmid-lipofectamine mixture for 6 hours, followed by fetal bovine medium for 48 hours. Immunohistochemistry was performed for transient expression. G418 was added after 48 hours.

①脂质体法：取体外分离、培养的第3代豚鼠骨髓间充质干细胞，将质粒-脂质体混合物加入含细胞的培养基中培养6 h，再加入胎牛血清的培养基，孵育48 h 后行免疫组织化学检测，即为瞬时表达。48 h后加入含G418培养基筛选。

Methods The expression of Galectin-3 was investigated by immunohistochemistry in the tissures of 50 thyroid cancers （including 32 papillary cancers and 18 follicular cancers）, 45 thyroid adenomas and 20 normal thyroids.

运用免疫组织化学方法，检测50例甲状腺癌（其中甲状腺乳头状癌32例，甲状腺滤泡状癌18例）、45例甲状腺腺瘤及20例正常甲状腺组织中Galectin-3的表达。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。