免疫化学

Methods: The immunohistochemical method was used to detect the expression of TGF-β1 and Cyclin Dl in the proliferating and involuting human hemangioma and normal skin tissues.

采用免疫组织化学方法检测人皮肤血管瘤增生期、退化期及其正常皮肤组织中TGF-β1、Cyclin D1的表达水平，并进行相关性分析。

The expressions of Bax and TGFβ1 in 50 children cases of dermal hemangioma which were in proliferative phase or in involuting phase were detected by HE staining and immunohistochemical method. We also analysized the difference between the different phases of hemangioma , and try to find out the role of Bax and TGFβ1 in hemangiomatous proliferation and regression .

采用HE染色和免疫组织化学方法检测50例小儿皮肤血管瘤增生期、退化期Bax、TGFβ1的表达水平，并分析它们在不同时期血管瘤中的差别，近而探讨Bax、TGFβ1在血管瘤增生、消退中的作用。

and materials:By immunohistochemistrical method, the expression of VEGF, c-myc and P53 were respectively observed in the tissue sections of 22 proliferative hemangiomas,23 involuting hemangi- omas and 16 vascular malformations.

材料和方法用免疫组织化学SP法检测22例增生期及23例消退期血管瘤及16例血管畸形组织中VEGF、c-myc及 P53蛋白的表达。

Each group was treated by intraperitoneal injection once every two days. The accumulation and metabolism of Al was analyzed by atomic absorption spectrum, expression of ED-1 by Kupffer cell was analyzed by immunohistochemistry.

采用原子吸收分光光度法测定铝元素在肝组织中含量的变化；免疫组织化学染色观察肝组织中枯否细胞的活化；RT-PCR检测凋亡相关基因Bax及Bcl-2 mRNA表达的改变。

The cell biological features were observed by inverted phase contrast microscope, l ight microscope, electron microscope, cell vital ity assay, cell growth curve and cells staining after harvest and during the periods of culturing the primary, the 1st passage and 2nd passage.

分别在取材后、原代、第1 代、第2 代细胞培养期间，进行髓核细胞活力测定；爬片培养后进行甲苯胺蓝、HE、聚集蛋白聚糖番红O、Ⅰ型及Ⅱ型胶原免疫组织化学染色观察；MTT 法绘制髓核细胞生长曲线，并行原代及第2 代细胞透射电镜观察，对体外细胞的生物学特性进行研究。

RESULTS: Light microscope showed that the collagen fibers arranged regularly in AECM with blankness of bone lacunas by HE, Mallory-Heidenhain rapid one-step dyeing and that the region around bone lacunas was stained different degrees of blue-green by Alcian blue dyeing.

结果 HE、Mallory- Heidenhain氏快速一步法染色后镜下可见AECM胶原纤维排列规则，骨陷窝空虚；阿利新蓝染色可见 AECM骨陷窝周边染色呈不同程度蓝绿色；免疫组织化学染色可见 FN和 L N的抗体染色阳性部位在细胞外基质。

The exemplary anti-cancer compositions of the present invention may also be used as adjuncts with one or more of the following: a surgical procedure, a laser therapy, a micro-radiation pellet implant, a cryogenic procedure, a chemotherapy, a radiation therapy, a electrodessication/curettage, a laetrile therapy, an immuno-therapy, and an enzyme therapy.

本发明技术抗癌制剂独特之处还在于可以与下列之一或多者结合使用：手术治疗、激光疗法、微放射颗粒植入、冷冻疗法、化学疗法、放射疗法、电烧法/刮除术、苦杏仁苷、免疫疗法和酶替代疗法。

Methods The SD experimental rats were divided into four ovarian stages basing on the vaginal lavages, from which the ovaries were obtained in every cycling stage.

利用阴道涂片的方法将实验大鼠分为四组不同的卵巢周期，采集不同时期卵巢标本，采用免疫组织化学方法观察卵巢中NR4A1蛋白的定位及表达情况。

Because of the low efficiency, only those detection methods that dont require too much cells, such as immunocytochemistry and RT-PCR, can be adopted in the traditional way, which limites the application of this differentiation model in science research.

传统ATRA法诱导分化的效率较低，只能采用免疫细胞化学或PCR等细胞需求量较小的方法进行相关检测，这样，就大大限制了这一分化模型在科研中的应用。

Breast tissues were taken at surgery from patients with breast adenoma, nodule of lobuli glandulae mammariae and breast cancer. Normal samples adjacent to breast cancer were used as control. NIS mRNA and protein were assessed in these samples by reverse transcription polymerase chain reaction , Western blot and immunohistochemistry methods.

1。乳腺组织取自乳腺纤维瘤、乳腺小叶增生以及乳腺癌患者，另取癌旁正常乳腺组织作为对照，分别运用半定量逆转录聚合酶链反应、Western Blot方法和免疫组织化学技术检测NIS的基因和蛋白表达情况。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。