免疫化学

PH values ; Fixatives ; Antigen retrievial buffers ; Immunohistochemistry ; Stains and staining ; Hydrogen ion concentration

pH值；固定剂；抗原修复液；免疫组织化学；染剂和染色法；氢离子浓度

Methods A total of 90 samples of thyroid tissues (60 thyroi d carcinomas, 10 thyroid adenomas, 10 goitres and 10 normal thyroid tissues) wer e studied by SP immunohistochemical method.

应用免疫组织化学SP法检测 6 0例甲状腺癌、10例正常甲状腺组织、10例结节性甲状腺肿及 10例甲状腺腺瘤组织中hTERT和P5 3的表达。

RESULTS AND CONCLUSION: Osteoblast was fusiformed-shaped and had plentiful processes. Nucleus was orbicular-ovate and leaning to lateral side. Soma was large, and plasma was abundant. Alkaline phosphatase staining suggested that a great number of gray-black particles were observed in plasma, and some region was darkly stained.

结果与结论：成骨细胞呈梭形，多突起；细胞核呈卵圆形，偏于一侧；胞体大，胞浆丰富；碱性磷酸酶染色可见胞浆中含有大量的灰黑色颗粒，某些部位染成黑色，定量分析细胞内的碱性磷酸酶、骨钙素水平明显高于成纤维细胞；细胞免疫组织化学染色表明其主要合成Ⅰ型胶原。

Groups were divided as follows: diabetic group, 1,25-2D3 treated group [1,25-2D3 group] and control group. At the 12 week, haematuria and proteinuria were determined and the kidneys were removed by histopathology, immunostaining, and the expression of Nephrin mRNA and protein in renal cortex were detected by reversed transcription-polymerase chain reaction.

建立糖尿病大鼠模型，实验12周末观察各组血尿、蛋白尿、肾组织光镜电镜、免疫组织化学，采用反转录聚合酶链反应检测其肾皮质Nephrin mRNA及蛋白的表达。

To defend the body, the immune system reacts, often by creating antibodies which release chemicals called histamines.

由于身体的抵抗力，免疫系统会进行抵抗反击，通常会增加抗原体来释放一些化学物质，通常被叫做组织胺。

Expression of hbFGF in the RPE cells was determined by in situ hybridization and immunohistochemical met hods.

于荧光显微镜下观察GFP的表达情况，用原位杂交和免疫组织化学染色法检测bFGF在RPE细胞的表达情况。

With the establishment of animal models of aortic homograft trans-plantation,using immunohistochemical staining,the differ-ence of the expression of MHCⅠantigen and MHCⅡanti-gen was detected between fresh aortic homografts and cryop-reserved aortic homografts.

建立大鼠同种异体主动脉移植模型，采用免疫组织化学实验研究新鲜主动脉和低温保存的主动脉同种异体移植后MHCⅠ类抗原和MHCⅡ类抗原表达的区别。

Results The signal intensity of infracted tissue on DWI images was markedly higher than that of normal brain tissue at one hour after stroke, and becaming higher and higher at the following three scan time.

测量ADC值、T2信号强度，根据CD34免疫组织化学染色结果计数毛细血管数，方差分析法分析不同时间点结果的差异，并进行相关性分析。

objective to investigate whether hypoxia preconditioning exist in adipose tissue,and construct the hypoxemic model of adipose tissue.methods after injected different density cocl2 to rat's subcutaneously of groin,use immunohistochemical method detect hif-1α protein,and use rt-pcr detect hif-1α mrna.results after injected different density cocl2,the adipose tissue of rat's groin express different degree of hif-1αprotein and hif-1αmrna.,and reach the peak when the density is 20-40 mg/kg and the time is 3 h.conclusion hypoxia preconditioning exist in adipose tissue,and the effect of preconditioning is most evidence when the density is 20-40 mg/kg and the time is 3 h.

目的 探讨脂肪组织中是否存在缺氧预适应现象，构建cocl2致缺氧的缺氧模型。方法不同浓度的cocl2大鼠腹股沟区皮下层浸润注射，分别用免疫组织化学法和rt-pcr法检测该区脂肪hif-1α蛋白和hif-1α mrna的表达情况。结果用不同浓度的cocl2行化学缺氧后在不同时间点观察有不同程度的hif-1α蛋白和hif-1α mrna表达，其中，在浓度为20～40 mg/kg，时间为3 h时脂肪组织中hif-1α表达达高峰。结论脂肪组织存在缺氧预适应现象；cocl2的浓度选择20～40 mg/kg，时间选择3 h时缺氧预适应效果较佳。

Methods: The immunohistochemical method was used to detect the expression of TGF-β 1 and Cyclin D1 in the proliferating and involuting human hemangioma and normal skin tissues.

采用免疫组织化学方法检测人皮肤血管瘤增生期、退化期及其正常皮肤组织中TGFβ1、CyclinD1的表达水平，并进行相关性分析。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。