免疫化学

Methods Samples of 46 cases with PCa were examined using immunohistochemical staining with TGF-β1 and β3 antisera.

采用免疫组织化学方法对46例PCa标本进行染色。

Methods Samples from 78 patients with pituitary adenomas, including 37 invasive and 41 noninvasive, and 5 normal pituitary tissue specimens from autopsied patients were examined. The expressions of OPN mRNA and OPN were examined by real-time fluorescence quantitative PCR and immunohistochemical SP method, respectively.

分别对78例垂体腺瘤手术标本（其中侵袭性37例，非侵袭性41例）和5例来源于尸检的正常垂体组织进行检测，采用实时荧光PCR技术检测组织中OPN mRNA表达，免疫组织化学SP法检测OPN蛋白表达，结合临床资料分析OPN mRNA及其蛋白的表达与垂体腺瘤侵袭性的关系。

Methods samples from 78 patients with pituitary adenomas, including 37 invasive and 41 noninvasive, and 5 normal pituitary tissue specimens from autopsied patients were examined. the expressions of opn mrna and opn were examined by real-time fluorescence quantitative pcr and immunohistochemical sp method, respectively. the relation of opn and opn mrna expressions to invasiveness of pituitary adenomas was analyzed.

分别对78例垂体腺瘤手术标本（其中侵袭性37例，非侵袭性41例）和5例来源于尸检的正常垂体组织进行检测，采用实时荧光pcr技术检测组织中opn mrna表达，免疫组织化学sp法检测opn蛋白表达，结合临床资料分析opn mrna及其蛋白的表达与垂体腺瘤侵袭性的关系。

Methods With in situ hybridzation to detect the expression and activity of telomerase. bcl|2 and bax were studied by strept Avidin|Biontin Complex immunohistochemistry.

用原位杂交方法检测端粒酶的表达及其活性，采用免疫组织化学技术SABC法检测bcl|2和bax。

Bcl|2 and bax were studied by strept avidin|biontin complex immunohistochemistry.results the expression rate of telomerase in breast cancer was significantly higher than that in precancerous breast(p＜0.01) and in breast benign tumors.

用原位杂交方法检测端粒酶的表达及其活性，采用免疫组织化学技术sabc法检测bcl|2和bax。结果端粒酶在乳腺癌中的表达率明显高于癌前病变（p.01）和乳腺良性肿瘤。

Objective To investigate the distribution of basophils in airway walls of fatal asthmatics, nonfatal asthmatics and non-asthmatics by using a novel monoclonal antibody.

目的 采用一种新的针对嗜碱性粒细胞的单克隆抗体，对不同程度支气管哮喘患者支气管肺组织进行免疫组织化学检测，以明确嗜碱性粒细胞在不同组织结构中的分布及其临床意义。

BE1 monoclonal antibody can be used to detect basophils in tissue.

BB1单克隆抗体可有效地用于支气管肺组织标本的嗜碱性粒细胞免疫组织化学检测，同时急性发作的致死性哮喘患者气道中嗜碱性粒细胞明显增加，可能与哮喘病情恶化、甚至死亡有关，而非致死性的慢性哮喘患者气道中嗜碱性粒细胞与对照组无异，说明嗜碱性粒细胞可能主要参与哮喘的急性病程。

To answer these questions, in the present study, an attempt was made to examine:(1) the GABA responses in mechanically dissociated Vme neurons with attached synaptic boutons by using the whole cell patch clamp technique;(2) the change of expression of KCC2 and NKCC1 during the postnatal stage in the Vme by using the Western blotting technique;(3) the percentage of colocalization of PV with KCC2 or NKCC1 during the postnatal stage in the Vme by using the double immunofluorescence histochemical techniques.

基于此，本论文进行了如下研究：(1)运用机械分离带终扣的单细胞膜片钳技术对大鼠Vme神经元对GABA的反应进行观察；(2)运用Western blotting技术结合图像分析方法观察生后发育过程中Vme神经元内NKCC1和KCC2的表达变化；(3)运用免疫荧光组织化学双重标记技术对生后发育过程中Vme神经元内NKCC1和KCC2与PV之间的共存关系进行了系统观察。

Methods: Myoblasts isolated from rat muscle samples were cultured and purified clonally in vitro. The 3rd passage cells were incubated with medium including basic fibroblast growth factor and dimethyl sulfoxid.

体外分离培养大鼠成肌细胞并克隆纯化，培养至第3代时加入含有碱性成纤维细胞生长因子和二甲基亚砜的培养液诱导分化，进行RT-PCR分析和免疫荧光细胞化学染色鉴定。

Methods Immunohistochemical staining were used to detect the expression and location of calpain in aging rat cochleas, and real time PCR assay were used to detect mRNA level of calpain and calpastatin in cochlear extracts.

采用免疫组织化学染色确定钙蛋白酶在老年大鼠耳蜗中的表达水平和组织定位，采用即时PCR技术分析耳蜗提取物中钙蛋白酶和钙蛋白酶抑素 mRNA水平的变化。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。