免疫化学

Methods Fifty white guinea pigs with normal hearing were randomly divided into the control group, ligustrazine group and traditional Chinese drug group. The alternations of hearing function were evaluated by the examination of auditory brainstem response. Some specimens were perfused by AgNO3 and observed the damage of bristle cell. And some were sent for immunohistochemistry to detect the expression of iNOS. Results Compared with the control group, the threshold of ABR in ligustrazine group and TCD group were tignificantly different.

将50只健康白色红目豚鼠随机分为对照组、顺铂组和中药组，各组动物均于用药前及停药后测试听觉脑干诱发电位，采用耳蜗铺片观察毛细胞损伤情况，应用免疫组织化学方法测定各组动物耳蜗内iNOS的表达情况。

RESULTS After treatment with CDDP at15mg L-1 ,there were apoptotic peaks in cell cycle analysis on FCM for48h and expression of MAPKs and transcripton factors in cell plasm and/or nucleus for72h.

结果 15mg L-1 顺铂作用肝癌SMMC-7721培养细胞48h可观察到明显的凋亡峰，72h免疫细胞化学染色发现胞质或胞核中MAPKs（JIN/SAPKs，p38MAPK，ERK）及转录因子均有明显表达。

Methods The chemical hypoxia was induced by incubation of U251 cells with cobalt chloride.

以氯化钴模拟缺氧，采用RT-PCR、免疫细胞化学方法分别检测缺氧诱导因子-1α和血管内皮生长因子mRNA和蛋白的表达，鸡胚绒毛尿囊膜模型检测缺氧时胶质瘤细胞血管生成情况。

Methods The expression of E-cd, MMP-2 and CD44v6 were examined in 50 patients with glioma and 9 cases of control brain tissues by immunohistochemi-cal assay.

采用免疫组织化学方法对50例神经胶质瘤患者和9例对照者脑组织中E-cd、MMP-2和CD44v6蛋白表达进行检测。

Prior to corneal infection, immunohistochemical staining demonstrated the constitutive expression of PBP in the cornea, lens and retina.

角膜感染前，免疫组织化学染色表明，PBP在角膜、晶状体和视网膜组成型表达。

This chemical is known to stimulate the immune system and help fight off disease-causing bacteria.

这种化学物质能启动免疫系统抵抗致病细菌。

The inhibition of SPG-Rg3 on the growth of MCF-7 cells was detected by MTT assay,IC50 was calculated to obtain the effective concentration; flow cytometry was used to observe the cell cycle of MCF-7 cells; AO/EB fluorescence double-dye technology was performed to observe the apoptosis of cells in morphology,immunocellularchemistry and RT-PCR were utilized to investigate the apoptosis of MCF-7 cells and its relationship with caspase-8 from protein level and molecule level.

采用MTT法观察SPG-Rg3对MCF-7细胞生长的抑制作用，并计算出IC50，依据IC50值确定SPG-Rg3的有效浓度；流式细胞术检测SPG-Rg3作用后MCF-7细胞周期的变化；AO/EB双染从形态学上观察SPG-Rg3对MCF-7细胞凋亡的作用；免疫细胞化学染色和RT-PCR技术分别从蛋白水平和分子水平上检测SPG-Rg3对MCF-7细胞的诱导凋亡作用及其与caspase-8基因的关系。

Control group was also set up.Then the AO/EB fluorescence double-dye technique,immunocytochemistry staining,Fluo-3 staining and Rhodamine 123 uptake assay approaches were adopted to observe the apoptotic rate,expression levels of Bcl-2 and Bax proteins,concentration of cytoplasmic free Ca2+ and mitochondrial transmembrane potential of NCI-H460 cells.

采用AO/EB荧光双染、免疫细胞化学染色、Rhodamine 123摄取法及Fluo-3/AM染色分别观察NCI-H460细胞的凋亡、Bcl-2及Bax蛋白的表达量、线粒体跨膜电位及细胞内游离钙离子浓度。

When we begin to the medicine, urine protein was measured in urine volume of 24 hour the every two day. 4. Specimen collections and laboratory check: In the 21 days of pregnant rats, infants were taken which registered weight and height after aesthesia. Cr and BUN in the blood were measured.

标本收集和实验室检查：于妊娠第21天在戊巴比妥钠(50mg/kg)麻醉下，剖宫取胎，记录胎仔体重，身长；同时抽取腹腔静脉血，分离血清以待测定血尿素氮，血肌酐；取左肾部分置于10%甲醛液浸泡固定，待用HE染色、PAS染色及免疫组织化学染色；另取部分肾脏剪成体积约1cm〓大小组织块，生理盐水冲洗至发白，-70℃保存。

Epilepsy rat models were made by injecting KA into amygdale under stereotactic instrument.The ERK1/2 protein expression in various groups were tested with immunohistological method.

采用立体定位仪下杏仁核内注射KA方法制备大鼠癫痫模型，于致痫后不同时程进行灌流固定、心肌组织的石蜡包埋、切片及免疫组织化学染色，检测不同时程心肌细胞ERK1/2表达的灰度值。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。