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免疫化学

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The tumor-bearing mice received corresponding treatments for 15 consecutive days, after which their thymuses and spleens were isolated and weighed to calculate the thymus and spleen indices. The tumor mass was also weighed and the tumor inhibition rate calculated.

连续用药15d。第15天处死小鼠,计算胸腺指数、脾指数;剥离瘤块称重并计算抑瘤率;用免疫组织化学方法检测各组荷瘤小鼠肿瘤组织的增殖细胞核抗原的表达强度。

Methods Adult male waster rats were randomly divided into the normal control pseudo-group and the experimental operation-group. In the operation-group, the right sciatic nerve was transected and survived for 1, 2, 4, 6 and 8 weeks after operation.

选取健康成年雄性Wister大鼠80只,随机分为假手术组和坐骨神经切断组,分别于术后1、2、4、6、8周五个时相处死后取患侧L4-L6背根神经节,应用Western Blot和免疫组织化学技术检测P-ERK1/2在背根神经节中的表达变化。

In this study, we explore the change on expression of Fas and FasL of promoting apoptosis at different period of time after spinal cord injury by making the model of transecting injury completely in SD rat and applying to the immunohistochemical method.

通过制备SD大鼠脊髓全横断性损伤模型,应用免疫组织化学ABC法,揭示促凋亡因子Fas和FasL与脊髓在完全性损伤后的不同时间点之间的相互变化趋势,以探讨Fas和FasL在脊髓损伤中的作用。

Methods: We transplanted neural stem cells from embryobrain of rats in hippocampi of AD model rats transecting fimbria fornix with or without FG. 30 days after transplanting, test these AD models' behavior then inspect living and differentiation of NSCs by immuno -fluorescence.

体外分离培养大鼠胚脑神经干细胞,用纤维蛋白凝胶为支架移植入穹隆海马伞切断AD模型鼠海马,行为学测试后用免疫组织化学方法检测神经干细胞存活和分化情况。

HSP70 existed in cytoplasm in normal condition, and predominantly translocated into nucleus after heat shock.

免疫细胞化学技术发现:L-02细胞在正常培养时,HSP70表达很低,且主要分布于胞浆;应激后表达明显增加且大部分分布于细胞核。

On the sixtieth postoperative day, the graft was harvested and the blood was taken from inferior cava venae. Histological changes were monitored by HE stain. The infiltration of CD4+ and CD8+ T lymphocytes and the expression ofα- smooth muscle actin were detected by immunohistochemistry.

术后60天取移植血管做病理组织学检查并利用计算机图象分析系统测量血管内膜厚度;免疫组织化学检测CD4+、CD8+T淋巴细胞的浸润及α-平滑肌肌动蛋白的表达;ELISA法检测血清中血管内皮生长因子的变化。

In the 56 , 22 were in sinus ventriculi, 6 in corpus ventriculi and 28 in fundus and cardia ventriculi.Tumoer size that was smaller than 5cm were 34 cases,while that was bigger than 5cm were 22 cases.

所有标本常规经10%福尔马林固定,石蜡包埋,定向切片;分别采用NF-κBp65鼠单克隆抗体和uPA兔多克隆抗体,以S-P法进行免疫组织化学染色,观察其在各组织标本中的表达。

Methods: Immunohistochemicl method was used to detect the expression of Ki -67 in human villi and GTD.

采用免疫组织化学的方法,观察正常早期妊娠绒毛和妊娠滋养细胞疾病组织中Ki-67的表达。

We talced depigmentized skin of 60 patients respectively before and after treatment by Chinese herb, contrasting with the vulvae skin of 20 healthy persons and observed their different expressions of reproduction antigen Ki-67 and human primary haematopoietic cell antigen CD34 in different skin tissue by immunohistochemical method.

观察组60例患者于治疗前后分别取患处皮肤与20例正常人外阴部皮肤对照,采用免疫组织化学的方法,分别检测增殖性抗原Ki—67以及人原始造血细胞抗原CD34在不同皮肤组织中的表达并进行观察。

Leukoplakia Vulvae;;Endothelial Growth Factors;;Tissues;;Blood Vessels;;Vulva;;Immunohistochemistry;;Skin

外阴白斑;;内皮生长因子;;组织;;血管;;外阴;;免疫组织化学;;皮肤

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