免疫化学

FDCS is a rare malignant tumor and differential diagnosis includes Langerhans cell sarcoma,interdigitating dentric cell sarcoma, malignant fibrous histocytoma, melanoma, metastatic spindle cell carcinoma and others.

正确诊断需要病理组织形态、电镜及免疫组织化学相结合，并应与朗格汉斯细胞肉瘤、指突状树突状细胞肉瘤、恶性纤维组织细胞瘤、黑色素瘤、梭形细胞癌等相鉴别。

The expression was higher in undifferentiated NPC than in keratinizing squamous cell carcinoma and non-keratinizing carcinomas.

用免疫组织化学方法检测CD25细胞在鼻咽癌组织和鼻咽慢性炎症组织中的表达情况。并用组织原位杂交技术检测EB病毒对其表达的影响。

Methods: Human luteinized granulosa cells were collected from patients undergoing in-vitro fertilization-embryo transfer.

收集体外受精－胚胎移植过程中的人黄素化颗粒细胞进行体外培养；应用RT-PCR法和免疫细胞化学法分别检测HB-EGF的mRNA和蛋白表达；应用实时RT-PCR法检测HB-EGF mRNA量的变化。

MSC induced by 5-aza could be identified by the positive staining for Troponin I and Connexin43. The number of cells stained positively in the 10umol L-1 and 20umol L-1 5-aza was visually greater than that of other groups , but more than 50% the cells necrosed in 20umolL -1 5-aza. Electron microscopy revealed sarcomeric organization and gap junction.

结果MSCs体外贴壁生长，呈成纤维细胞样形态，经5一aza诱导后细胞体积增大，变为球状或者长杆状，4w后荧光免疫细胞化学方法检测肌钙蛋白、肌动蛋白及Connexin43表达阳性，10umol·L一'5一aza组及20umoz·L一'5一aza组阳性表达较高，但20umol·L一'5一aza组约50%细胞死亡。

Material and Method: 40 paraffins of prostate carcinoma from 1996 to 2005 were collected.

材料和方法：搜集昆明医学院附属第一院和第三院1996年～2005年间临床资料完整的40例未经治疗前列腺癌患者的前列腺癌组织标本，应用免疫组织化学方法检测雄激素受体的表达。40例病例根据肿瘤的组织学形态进行病理分级。40例病例术前均行了盆腔前列腺CT扫描。

After natural purification and three passaging, we detected CD90 expression in fibroblasts grown on cover glass by SABC immuocytochemistry techniques.

经过3次传代、自然纯化后得到性质稳定的成纤维细胞，冻存后复苏传1-3代，制成细胞爬片，免疫细胞化学SABC法染色检测CD90的表达情况。5。

The lung tissue was cut to 4μm sections, deparaffinized and rehydrated, which were subjected to immunostaining using avidin -biotin -complexed method according to standard procedures.

取厚4μm的肺组织切片经过脱蜡、水化，严格按照免疫组织化学ABC试剂盒说明书操作。

Clinical files were available for three of them, and we found nuances in the immunohistochemistry from these previous reports. Here, we present these rare sarcomas and review the literature.

我们发现与以往文献上些许不同的免疫组识化学染色差异，在此我们提出这罕见的肿瘤，并回顾一下文献纪录。

Most 5-HT cells appeared in the base part of gland in the rat, while in the human they were mainly located in the intercellular substance and a few scatted in the glandular epithelium.

方法采用免疫细胞化学方法，检测人和大鼠胃窦部粘膜内生长抑素细胞、胃泌素细胞、5-羟色胺细胞（5-HT细胞）、嗜铬粒素A细胞的分布。

Immunohistochemistry results showed that the gray of PC12 cell cytoplasm appeared shallowing after HFS, suggesting that tyrosine hydroxylase activity decreased; The numbers of visible mitochondria in the cytoplasm, secretory granules, processes and synapse were decreased.

结果：相比对照组，HFS后PC12类神经细胞光镜下突起长度显著缩短，突起个数明显减少；酪氨酸羟化酶免疫组织化学结果显示HFS后PC12细胞胞质灰度降低，提示酪氨酸羟化酶代谢水平下降；电镜下可见胞质中的线粒体、分泌颗粒减少，突起和突触减少。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。