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免疫化学

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The expression of AT1 and AT2 receptor was examined in ureteric bud cell of the embryonic mouse, including embryonic days 12, 14, 15, 16 by immunohistochemical technique.

采用免疫组织化学方法检测胚龄12,14,15,16 d小鼠肾脏发育早期输尿管芽分支中血管紧张素Ⅱ受体1和受体2的含量。

Results; The histological change of kidney iNOS was markedly increased in the IR+NS group. The levels of serum creatinine and blood uria nitrogen were significantly upregulated.

应用免疫组织化学方法和图像分析系统检测肾组织中INOS的表达和分布,比较各组血清肌酐、尿素氮,同时观察肾脏组织形态学改变。

Key Words: Uronology;prostatic carcinoma;P504S;p63;CK34βEl2;PSA; immunohistochemistry

泌尿外科学;前列腺癌;P504S;p63;CK34βEl2;PSA;免疫组织化学

Methods Western blotting and immunohistochemical staining were used to detect CD147 expression in cervical cancer or normal cervix uteri tissues.

应用蛋白印迹及免疫组织化学方法检测CD147在宫颈癌和正常宫颈组织中的表达。

Methods The expressions of TNF-α and TGF-β1 proteins in uteroplacental tissues were detected with immunohistochemistry in 28 early spontaneous aborters and 28 normal early pregnant women.

采用免疫组织化学技术半定量测定28例早期自然流产患者和28例正常妊娠妇女绒毛和蜕膜组织内TNF-α及TGF-β1表达强度。

Methods The expressions of IGF-1R were detected by immunohistochemistry from 41 cases of uveal malignant melanoma.

用免疫组织化学SABC法检测IGF-1R在41例葡萄膜恶性黑色素瘤组织的表达。

METHODS We performed immunohistochemical staining on 192 circumferential venoatrial segments (32 veins) harvested from 8 autopsied human hearts using antibodies to tyrosine hydroxylase and choline acetyltransferase.

研究者利用酪氨酸羟化酶及乙酰胆碱转移酶抗体对从8名尸解的人体心脏中采集的192例肺静脉同左心房连接处周缘的肌肉片断(32个静脉)进行免疫组织化学染色。

Results as follows;(1) At 25th week of fetal age, the staining intensity and cell density almost equalled to that at birth in cervical spinal cord, brainstem, hippocampus, and vermis of cerebellum; while there are only 1/4 cell densities in cerebral cortex.

用胶质原纤维酸性蛋白抗体进行免疫组织化学染色。结果表明:(1)在颈段脊髓、脑干、海马和小脑蚓部于胚胎25周其GFAP染色强度、细胞密度接近出生时水平。

Key words: Schwann cell; Wallerian degeneration; artificial nerve; tissue engineering

10 d龄SD大鼠坐骨神经进行结扎预变性,分别于术后24 h、3 d、5 d、7 d和9 d,进行雪旺细胞培养和S-100免疫组织化学染色观察。

Results: Dynamic observation with histopathology and immunohistochemical staining showed that, after the nerve root nerve root compression, the myelinated nerve fibers decreased and there was ultrastructure changes of the nerve fibers, such as Schwann cell cytoplasm swelling and edema, Wallerian degeneration, and demyelinated degeneration of axons.

结果:通过对压迫神经根及神经根节大体观,组织病理学和免疫组织化学的光学显微镜的动态观察,可见神经根压迫后其有随神经纤维数目减少和超微结构的改变,如神经模细胞胞质肿胀和细胞水肿、华勒变性、神经轴突脱髓鞘变等。

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