免疫化学

Methods: the expression of hALK in HepG2 cells was observed immunocytochemistry and reverse transcriptive PCR. Proliferation of HepG2 cells after neutralization was detected by 3H-TdR incorporation approach.

用免疫细胞化学以及RT-PCR的方法检测HepG2细胞hALR蛋白及mRNA的表达；用3H-TdR掺入法检测hALR特异性单克隆抗体中和hALR后HepG2细胞的增殖情况。

Methods Expression of MMP-8, collagen Ⅰ and collagen Ⅲ in fascia transversalis from the patiens of indirect inguinal hernias and non hernias was examined by immunohistochemical method.

采用免疫组织化学方法检测30例腹股沟斜疝患者及25例非病患者腹横筋膜中MMP-8、胶原Ⅰ、胶原Ⅲ的表达。

Materials and methods: The lung preparation of 6 SARS death patient (died in 9,14,20,29,33,38d) and 6 macaque model (killed in 7,12,14,14, 32, 35d)were objects. Pathological changes, collagen fibers, lattice fibers, elastic fibers, collagen I and III in lungs and fine structure changes were studied by routine H.E dyeing, trigeminy dyeing, trinitrophenol- sirius red staining and polarization microscope, electron microscope and image analysis. Expression of Vimentin、 TGFβ_1、 TNF α、 IL-1β and MMP-2 were detected by immunohistochemistry.Results:1. Pathological changes of SARS death patient.

材料与方法：以6例SARS死亡患者(分别于发病后9、14、20、29、33、38d死亡)和6只猕猴实验模型(分别于染毒后7、12、14、14、32、35d活杀取材)肺标本为对象，应用H.E染色、三联染色、苦味酸-天狼猩红-偏振光法、电镜和图像分析等技术，对比性观察SARS肺组织病理变化和纤维化的病理过程、胶原纤维、网状纤维和弹力纤维的变化特点、Ⅰ型与Ⅲ型胶原纤维的数量和分布规律，旨在探讨SARS肺纤维化的病变经历过程及特点；利用免疫组织化学和形态计量学技术检测SARS死亡患者肺脏的Vimentin、TGFβ_1、TNFα、IL-1β和MMP-2等与炎症反应和纤维化相关活性因子，探讨其发病机制。

Materials and Methods A total of 65 patients with gastric carcinoma were evaluated with triphasic spiral CT with water filling method and hypotonic agent.

资料与方法对65例胃癌行低张力水充盈螺旋CT三期增强扫描，所有病例均行手术切除，术后标本采用免疫组织化学SP法检测肿瘤组织中nm23-H1蛋白表达。

Methods Endogenous EB1 mRNA and protein levels in embryonic cells of trophonema from artificial abortion and spontaneous abortion were compared, using fluorescence quantitative PCR, immunohistochemistry and western blot, respectively.

采用荧光定量PCR技术检测EB1基因在人工流产绒毛核型分析正常的胚胎细胞和自然流产绒毛核型分析异常的胚胎细胞中的表达差异；免疫组织化学方法检测EB1蛋白在两者中的表达情况，并用Western blot加以验证。

The FosB/△FosB positive staining was detected in nucleus accumbens septum, tuberculum olfactorium and dorsal caudate putamen, without little cFos positive staining. The results indicate the expression of cFos and FosB/△FosB display the differences in area and intensity, and the area intensively expressing FosB/△FosB is more nearly coincide with the area related to schizophrenia. So FosB/△FosB is a better mark to research the pathogenesis of schizophrenia.

同时大量研究证明，动物模型的行为及生理变化与中枢神经系统神经元的病理变化有关，因此，利用即早基因反应的灵敏性、功能多样性、检测方便性等，应用免疫组织化学的方法对模型小鼠脑内cFos和FosB/△FosB蛋白的表达进行检测，通过确定不同脑区cFos和FosB/△FosB蛋白的表达，来研究哪些脑区或神经元参与了反应，从而发现精神分裂症在脑区内所涉及的解剖结构，有助于对精神分裂症发病机制的认识。

Sections from frontal lobe, occipital lobe, striatum and hippocampus of normal subjects and sections from hippocampus of AD patients were used in hematoxylin eosin, Lox Fast blue, toluidine blue stains and ulex europaeus agglutinin immunostaining.

应用荆豆凝集素(ulex europaeus agglutinin， UEA)免疫组织化学及甲苯胺蓝等染色观察正常增龄病例额叶、枕叶、壳核、海马及AD患者海马的神经元、毛细血管形态改变，应用图像分析技术测算各部位神经元与毛细血管密度比值并分析其与年龄之间的相关性。

It was found that after differentiation the cells expressed the endocrine markers insulin 1, glucagons, somatostatin by RT-PCR, however, these endocrine markers cannot be found in uninduced cells.

通过免疫细胞化学染色可以检测到在ICCs中存在有胰岛素、胰高血糖素、生长抑素和pdx-1阳性的细胞，通过RT-PCR可以在mRNA水平检测到胰岛素、胰高血糖素、生长抑素、pdx-1、ABCG2、葡萄糖转运蛋白等的表达。

We used the immunohistochemical ABC method to detect the expression of NF-κB at 2、 6、 12、 24、 48、 72 hours after infected by Salmonella typhimurium, and we deal the data statistically with univariant analysis of variance and Student-Newman-Keuls multiplerange test.

三组分别在感染鼠伤寒沙门氏菌后2、6、12、24、48、72小时6个时点，用免疫组织化学方法(抗NF-κB p65抗体、ABC法，石蜡包埋)和阳性细胞面积分布率半定量测定小肠、结肠及肾脏中NF-κB活性表达百分率，采用单变量多因素方差分析和Q检验做统计学处理。

The findings of the newly identified goose paramyxovirus strain GPMV/HBexperimental infection were as follows: The challenge caused high morbidity and highmortality.The clinical signs included severe depression, decreased appetite or anorexia,white,yellow and green diarrhea, with neural symptoms;pathological changes showed thathaemorrhage and ulceration of intestine mucosa, necrosis of spleen and pancreas,andedema of liver and kidney, uratic deposition in kidney.Eosinophilic inclusion bodies werefound in the cytoplasm of pancreas and a large quantities of fiber protein were found inintestine. Lots of hemosidefin was found in some area of liver and kidney. A number ofT、B lymphocytes reduced sharply in spleen,lymph nodes in dead geese. Transmissionalelectron microscope showed that some cells had the morphological characteristics ofmitochondria swelling, morphological changes were condensation of nuclear chromatininto dense masses,followed by its marginating against the nuclear envelope, andespecially concentration of cytoplasm association with lots of lipid droplet.

人工感染雏鹅实验中，雏鹅临床表现为高发病率和高死亡率，食欲和饮水减少，拉白色、黄绿色稀粪，伴有神经症状；常规组织学观察，脾脏和胰腺肿大，有白色坏死斑点，肠道出血和坏死，肝脏肿大变性，肾脏肿大变性有尿酸盐沉着等；用特殊染色和组织化学方法首次对人工感染鹅副粘病毒的雏鹅的重要脏器进行研究，结果表明：胰腺中发现病毒包涵体，肠道中大量纤维素以及胶原纤维增生，肾脏、肺脏大量含铁血黄素沉着，免疫器官如脾脏和淋巴结中T、B淋巴细胞大量减少；电镜下多种细胞呈现线粒体水肿，多种实质细胞的染色质固缩，染色质边集，特别是细胞内容物如脂肪滴增多的病理变化特征。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。