免疫化学

Immunohistolochemical stain by monoclonal antibodies for growth factors and actin was used to detect whether there are abnormal expressions in saccular aneurysms.

利用血小板源性生长因子、转化生长因子（TGF-β1）、血管内皮细胞生长因子和肌动蛋白的单克隆抗体进行免疫组织化学染色。

Methods:Thirty six mice were inoculated with the sarcocarcinoma 180 according to the standard method,after 10 days,weighed the tumor and body.The expressions of bcl2 and p53 proteins in the tissue were studied by immunohistochemistry; and the activities of SOD, GSHPx and the content of MDA were detected by colorimetric method in serum.

按照标准方法给36只小鼠接种S180肉瘤后，给予珍珠梅提取物10 d，观察瘤重及体重，免疫组织化学方法检测肿瘤组织中的bcl2和p53蛋白，比色分析法检测血清SOD，GSHPx的活性及MDA含量的变化。

Methods:Thirty six mice were inoculated with the sarcocarcinoma 180 according to the standard method,after 10 days,weighed the tumor and body.The expressions of bcl 2 and p53 proteins in the tissue were studied by immunohistochemistry; and the activities of SOD, GSH Px and the content of MDA were detected by colorimetric method in serum.

按照标准方法给36只小鼠接种S180肉瘤后，给予珍珠梅提取物10 d，观察瘤重及体重，免疫组织化学方法检测肿瘤组织中的bcl 2和p53蛋白，比色分析法检测血清SOD，GSH Px的活性及MDA含量的变化。

Dynamic expressions of TNF-α were observed in the DM rats' sciatic nerve by immunohistochemical methods.

应用免疫组织化学的方法观察TNF-α在大鼠下肢坐骨神经中的动态表达规律。

Methods: Immunohistochemistry was applied to examine the expression of PTEN, P27 and cyclin D1 in 58 cases of human colorectal carcinoma, 58 cases of tumor sideward mucosa and 14 cases of normal colorectal mucosa.

应用免疫组织化学方法检测58例大肠癌组织、58例癌旁组织和14例正常大肠黏膜中PTEN、 p27和cyclin D1蛋白的表达情况。

Positive expressive rate of cyclin D1 in colorectal carcinoma was 60.34%（35/58）, which was higher than that in tumor sideward mucosa and normal colorectal mucosa.

应用免疫组织化学方法检测58例大肠癌组织、58例癌旁组织和14例正常大肠黏膜中PTEN、 p27和cyclin D1蛋白的表达情况。

Methods : MTr assay was used to examine the effect of Sorbaria sorbifolia on the proliferation of HepG-2 cell and flow cytometry was used for the cell cycle distribution.

采用MTT比色法观察珍珠梅提取物抑制肝癌HepG-2细胞增殖；采用流式细胞仪检测其对HepG-2细胞周期分布的影响，同时结合倒置显微镜、HE染色、透射电镜及免疫细胞化学方法检测凋亡相关基因bcl-2、p53的蛋白表达，观察其诱导肝癌HepG-2细胞凋亡的作用。

The inducible nitric oxide synthase is obviously expressed in speen of rats under the predator stress, suggesting important function for iNOS in speen physiology. However, the expression and subcellular localization for NOS in speen development and differentiation are largely unknown. In the present study, the changes in iNOS activity in spleen of rats under predator stress were studied to explore the role of iNOS in the decreased immunoactivity of the stressed rats. The changes in iNOS were observed with immunobistochemical method, and the contents of NOS and NO in spleen with biochemical method.

在捕食压力下，大鼠脾脏诱导型一氧化氮合酶明显表达，说明iNOS起到了重要的生理功能，然而，NOS的表达及在亚细胞水平的定位以及相应的差异变化等都很不明确，本试验采用捕食应激模型，利用免疫组织化学方法和生化测定，检测心理性应激对大鼠脾脏iNOS活性的影响及探讨这种影响与应激动物免疫力下降之间的关系。

Localisation of testosterone and androgen receptors was investigated in different stages of the spermatogenetic cycle of Rana chensinensis, using immunocytochemistry.

用免疫细胞化学方法检测了睾酮和雄激素受体在中国林蛙生精周期中不同时期精巢内的表达定位。

Then rAAV-DJ-1 was transfected into HEK-293 cells for packaging. AAV vectors encoding human DJ-1 protein were stereotactically injected into the rat SN 4 weeks prior to rotenone lesion in SN. Tyrosine hydroxylase protein was determined using immunohistochemical staining. Ethovison software allowed measurement of movement distance in 30 minutes.

并转染HEK-293细胞，进行腺相关假病毒颗粒包装，所得病毒颗粒注射到大鼠脑内感染黑质神经细胞；4周后，注射鱼藤酮于大鼠黑质相同脑区；通过免疫组织化学方法鉴定TH蛋白表达情况，采用动物行为轨迹分析软件计算大鼠30 min内运动距离。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。