免疫化学

The immunohistochemical method was used to detect Fos expression in Central Nerve System induced by pulpitis pain.

应用免疫组织化学方法检测牙髓炎疼痛诱发中枢神经系统（central nerve system， CNS）c-fos的表达。

MTA was used to manage clinical problems. These included pulpless permanent teeth with incomplete root formation and perforation in roots or furcations. Total 10 cases (12 teeth), former 7 cases (7 teeth) and latter 4 cases (5 teeth), were present. The follow-up periods were various from 6m to 21m.

应用免疫组织化学手段，观察细胞外非胶原蛋白骨唾蛋白（bonesialoprotein，BSP）、骨桥素（osteopontin，OPN）和骨钙素（osteocalcin，OC）在根尖周组织愈合期间的表达，尤其是在与倒充填材料直接相邻区域的表达。

Methods: The fibroblasts companying human hepatoma were primarily cultured with explant culture method, first passaged after they spread to the bottom of the culture bottle, and pured by enzyme digestion and repeated strapping the cells were identificated through observing the morphologic change using invert microscope and detecting expression of vimentin, keratin by iMmunohistochemical method.

应用组织块培养法进行人肝癌伴生成纤维细胞的原代培养，细胞铺满培养瓶底后首次传代，通过胰酶消化法和反复贴壁法进行成纤维细胞的纯化；通过倒置显微镜观察细胞形态、免疫组织化学方法检测细胞膜波形蛋白、角蛋白表达情况，对细胞进行鉴定。

Fig.1 SHEE cultured on coverslide, the living cells were growing in single layer with rich cytoplasm, the nuclei were uniform in size with a nucleolus ph ×400 Fig.2 SHEE had a nucleus with ellipse shape, large nucleolus and the cytoplasm contained mitochondria and tonofibrilEM ×10 000 Fig.3 SHEE was spherical in shape, with pseudopods attached on petri dish and abundant villi on cell surface SEM ×5 000 Fig.4 Same as in Fig.3, cell attached on petri dish, appeared stellate or polygonal in shape, with abundant pseudopods and cytoplasmic processes. Protrusive nuclear region in central part of the cell had more micro-villi SEM ×5 000 Fig.5 Chromosomes of SHEE cells belonged to diploidy type Giemsa ×1 000 Fig.6 The SHEE cells of stained in dark brown by Ki67 immunohistochemistry were the proliferative cells Immunohistochemistry ×400 Fig.7 In SHEE cell culture, the nucleus stained red or pink by PI was dead cell, the green nucleus was living cell Fluorescent ×400 Fig.8 The cell labeled by TdT was apoptotic cell in which the chromatin of nucleus condensed in block, a pyknotic nucleus in the upper right conner was seen TdT labeled ×400

图1 SHEE培养在盖坡片上，活细胞单层生长，胞浆较丰富，细胞核大小一致，有核仁×400 图2 SHEE培养细胞细胞核椭圆形，核仁较大，胞浆有较丰富的线粒体和张力原纤维EM ×10 000 图3 SHEE细胞呈球状，有伪足贴壁，表面有密集微绒毛SEM ×5 000 图4 同上细胞贴壁，呈星状或多角形，有丰富伪足和胞浆突，核区隆起有较多微绒毛SEM ×5 000 图5 SHEE细胞染色体仍属二倍体Giemsa染色×1 000 图6 SHEE细胞Ki67免疫组织化学染棕黄色为增殖细胞×400 图7 SHEE培养细胞出现死细胞，胞核和胞浆PI染色呈红色或淡红色，蓝色细胞核为活细胞荧光显微镜×400 图8 细胞TdT标记阳性为凋亡细胞，染色质凝集呈块状，右上角有一固缩细胞核TdT标记×400

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育，MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用；RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1)，以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况；Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达，以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用；免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达；激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位，并观察真菌刺激后人角膜上皮细胞骨架的变化；流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变；光学显微镜观察真菌与人角膜上皮细胞黏附数量，记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后，细胞超微结构的改变。

The rate of abnormity was30.29%.Conclusion The sensitive and accuracy and spe-cialties of the detection in BALP confirmed good by clinic diagnosis of rachitic,superior to other methods and index.

用全血干化学免疫浓缩法对307例新生儿进行骨碱性磷酸酶活性测定，采用多元线性及逐步回归法分析新生儿BALP活性水平与母亲妊娠因素的相关性。

And52examples that between250and300u/L.The rate of abnormity was30.29%.Conclusion The sensitive and accuracy and spe-cialties of the detection in BALP confirmed good by clinic diagnosis of rachitic,superior to other methods and index.

用全血干化学免疫浓缩法对307例新生儿进行骨碱性磷酸酶活性测定，采用多元线性及逐步回归法分析新生儿BALP活性水平与母亲妊娠因素的相关性。

Objective: to investigate the mechanism about the effect of danshensu on the transforming growth factor beta 1 signaling pathway in Rattus norregicus hepatic stellate cells .

以肝星状细胞株HSC-T6为研究对象，免疫细胞化学法观察丹参素对TGFβRII表达的影响，RT-PCR法检测丹参素对Smad2和Smad4表达水平的影响，检测结果做图像分析处理。

Central nervous system neoplasms ; atypical teratoid/rhabdoid tumor ; child ; immunohistochemistry

中枢神经系统肿瘤；非典型畸胎瘤样/横纹肌样瘤；儿童；免疫组织化学

He experiment results indicate that (1) The extract of Rubia Cordifolia L has the effects of anti-proliferation and inducing APO;(2) Whenadministrating with 5-fu, the extract of Rubia Cordifolia L obviously enhanced the effects of anti-proliferation and inducing APO of 5-fu;(3) The basement of molecular biology of the extract of Rubia Cordifolia L for inducing APO of MGC-803 cells is correlated with inhibiting the expression of bcl-2 of anti-apoptosis gene.

UNEL法与PCNA的免疫细胞化学法的结果：0.2、0.4、0.8、1.0mg/ml浓度的茜草提取物与2、4、8、16μg/ml浓度的5-Fu，分别诱导细胞凋亡和抑制细胞增殖，表现出浓度、时间依赖性关系，除个别浓度与个别时间之间无显著性差异（P＞0.05）外，不同浓度与不同作用时间之间均有显著性差异（P＜0.05或P＜0.01），并且两种药物联合使用时，MGC-803细胞的APO%显著提高，降低PCNA的阳性表达率（P＜0.05或P＜0.01）。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。