免疫化学

In situ hybridization and immunocytochemical studies indicated that treatment with 17beta -estradiol increased the number of aromatase-immunoreactive cells and their immunoreactivities, especially in the preoptic area of the hypothalamus, where the density of the aromatase-immunoreactive cells was 2.6 folds higher than the control group. Results indicated that brain aromatase may be involved in the feminization induced by 17beta -estradiol of the grey mullet.

原位杂交和免疫细胞化学定位表明：雌二醇处理组鲻鱼脑内芳香化酶阳性细胞数量和反应强度均提高，尤其在下丘脑视前区，芳香化酶阳性细胞的分布密度为对照组的2.6倍，说明17beta-雌二醇诱导幼鲻雌性化可能与脑芳香化酶的表达有关。

So a definitive diagnosis of this eneity relies very much on histopathology, aided by clinical history, presurgical serum level of calcium, and exclusionary immunohistochemistry.

因此最可靠的鉴别更多的依赖于组织病理学，借助于临床病史、外科手术前的高血钙和免疫组织化学的排他性诊断。

The chemical constituents from Primulaceae plants are mainly flavonoids, terpenoids, sterols, organic acids, quinines, etc. These plants have effects of lowering serum uric acid level in mice, promoting bile secretion in rats, reducing bile pigment stones formation rate, immune regulation and so on.

报春花科植物中主要化学成分有黄酮类、萜类、甾醇类、有机酸类、醌类等；药理活性主要有降低小鼠的血清尿酸水平，促进大鼠胆汁分泌，减少胆色素结石的成石率，免疫调节功能等。

He volume of cerebral infarction was calculated by TTC staining, the expressions of NF-κB、Bcl-2 and Bax proteins in procerebrum cortices and basal nuclei of rats were detected by immunohistochemistry.

TC染色法测量脑梗死体积，免疫组织化学方法检测前脑皮质、基底核NF-κB、Bcl-2和Bax蛋白的表达。

This experiment aimed to systematically investigate and analyze the ethanol activated rates of mouse oocytes, affected factors of oocytes activation, types and development of parthenotes, and the organization and roles of the cytoskeleton during pronuclear formation and migration and early embryo cleavage by ethanol activation of oocytes, embryo culture in vitro and immunofluorescence cyto-chemistry. The results:(1) The ethanol activation of mouse oocytes showed that the activated and developmental rates of oocytes increased significantly with the increase of ethanol concentration and extension of exposure time, but over concentration and exposure time would result in increased fragment rates significantly. 7% ethanol treated oocytes for 7min was the optimum activated condition.

本实验主要利用卵母细胞的乙醇激活、胚胎体外培养和免疫荧光细胞化学方法对小鼠卵母细胞乙醇激活效率、影响因素、孤雌胚类型、发育、原核形成、迁移及早期胚胎卵裂过程中细胞骨架的组装、作用等进行了系统的研究和分析，结果显示：(1)小鼠卵母细胞的乙醇激活结果表明，随着乙醇浓度的升高和作用时间的延长，卵母细胞激活率和发育率都显著提高，但乙醇浓度过高和作用时间过长会导致卵母细胞碎裂率的显著增加，7%乙醇作用卵母细胞7min 为最佳激活条件。

HE's photomicrographs were analyzed for morphology. The proteinic expression of cyclophilin A was observed by immunochemistry. The mRNA's amount of ICAM-1 and P-selectin were quantitated by real-time quantitative reverse-transcript PCRs. All replicate data are expressed as mean±SEM.

每组随机取4例标本通过HE染色切片进行血管形态学分析，并使用免疫组织化学染色方法分析亲环素A在蛋白质水平的表达情况；另每组随机取7只小鼠通过实时定量反转录方法检测ICAM-1与P-选择素mRNA的表达。

Methods: HPV 16/18 infection was detected by nested-polymerase chain reaction, the p53 mutation was detected by direct sequencing, and the p53 and the HPV 16/18 E6 proteins were studied using immunohistochemistry on 129 pterygial specimens and 20 normal conjunctivas.

本研究利用巢叠式聚合脢锁反应分析自129例罹患翳状赘肉之翳状赘肉组织及20例正常无翳状赘肉的结膜组织检体中，是否有人类乳突病毒16及18型的感染，并利用直接定序的方式检测p53基因是否发生突变，此外亦以免疫组织化学染色法侦测p53蛋白与人类乳突病毒E6蛋白。

MethodsPrimary nasal pterygial tissues(15 eyes) and normal human conjunctival tissues(8 eyes) were determined by immunohistochemical staining, using monoclonal and polyconal antibodies specifically against MMP-3, LN and col Ⅲ. The intensity of reaction on individual sections was semiquantitatively graded.

方法应用S-P免疫组织化学染色方法检测15例翼状胬肉组织及8例正常球结膜组织中MMP-3、LN、col Ⅲ的免疫学定位及表达情况，用Masson及VG染色法检测各标本中胶原纤维及弹力纤维的分布情况。

Methods: The expression level of NOS and NGF was detected in pterygopalatine ganglion of the allergic rhinitis rats using histochemieal and immunofluorescence staining methods respectively.

建立AR大鼠模型，用HE染色方法观察鼻黏膜内腺体的变化；用组织化学的方法观察翼腭神经节内NOS表达的变化；用免疫荧光的方法观察翼腭神经节内NGF表达的变化。

Methods Immunohistochemistry method was used to stain the substance P in pulpal tissue which could directly reflect the activation of nerve by stimuli.

应用免疫组织化学染色的方法，对树脂充填深龋洞后不同时期P物质表达变化进行观察研究。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。