免疫化学

RESULTS: High-purity ADSCs were acquired successfully. The ADSCs were induced into osteoplastic differentiation, and displayed typical adipocytes changes 7 days after induction. the cells turned to slender fusiform and mixed together, which showed positive PPARγ immunohistochemical staining and the lipid droplet was stained orange-red by oil red staining.

结果：①成功获得纯度较高的脂肪基质细胞，成功诱导其脂向分化，诱导培养后7 d观察到细胞形态学上表现出典型的脂肪细胞改变：细胞相互融合，成片生长，变为细长纺锤形；PPARγ免疫细胞化学染色呈阳性；油红O染色脂滴被染成橙红色。

Five sheep were sacrificed at 3, 4 and 6 weeks after osteotomy, respectively, and specimens were harvested for detecting the expression of TGF-β1 and IGF-I by immunohistochemistry and RT-PCR.

术后3、4和6周分别处死5只动物，应用免疫组织化学与RT—PCR检测骨痂组织中TGF—β1与IGF—Ⅰ的表达。

Methods The PCO rat models, prepared by pregnancy hormone combine with human chorionic gonadotrophin, randomly divided into four group as control, model, Chinese crude drug and western medicine group; The levels of serum gonadal hormone were detected by radioimmunoassay; The effects of ZYC on ovaries and ovarial follicular development were observed by light microscope; And the expression level of TNF-α was detected with the immunohistochemical staining technique.

采用孕激素联合人绒毛膜促性腺激素造模法制备PCO模型大鼠。随机分为对照组、PCO模型组、PCO模型组+助孕胶囊组、PCO模型组+克罗米酚组，运用放免法测定血清性激素水平的变化；光镜观察卵巢、卵泡发育的影响，免疫组化学法检测卵巢组织肿瘤坏死因子α表达水平。

The positve rates in serous carcinomas and borderline tumors were 39.3% and 55.6%, significantly lower than that in benign tumors and normal ovaries (P.05), but the statistical differences were not found between borderline tumors and serous carcinomas, benign tumors and normal ovaries.2 WWOX mRNA and protein expression in ovarin seruous tumors2.1 WWOX mRNA expression in ovarin seruous tumors The result of RT-PCR showed that WWOX mRNA expression were detected in 29 of 32 ovarian serous benign tumors, 12 of 16 ovarian serous borderline tumors, and 21 of 45 ovarian serous carcinomas respectively.

阳性条带密度扫描定量结果，卵巢浆液性癌中PTEN蛋白表达相对量为0.34±0.12比正常卵巢和浆液性囊腺瘤和交界性囊腺瘤明显降低(p.05)，其余各组间无统计学差异。3.2.2 PTEN蛋白FCM检测结果FCM结果，卵巢浆液性癌PTEN蛋白表达的FI值(0.908±0.023)明显低于正常卵巢、卵巢囊腺瘤和交界性囊腺瘤的FI值，差异有统计学意义(P.05)。3.2.3 PTEN蛋白免疫组织化学结果35例浆液性囊腺瘤、18例交界性囊腺瘤和56例浆液性癌中，分别有31例、15例和29例呈阳性表达。

The aim of this study was to investigate the expression of mPGES-2 protein in mouse oviduct during the early pregnancy, pseudopregnancy, and estrous cycle by immunohistochemistry and Western blot. The regulation of mPGES-2 protein in mouse oviducts was also studied under delayed implantation, steroid hormonal treatments and oviductal ligation.

本实验主要利用早期妊娠、假孕、输卵管结扎、发情周期、激素调节以及延迟着床等实验模型，结合免疫组织化学和Western blot 的实验方法，详细研究了mPGES-2 蛋白在小鼠输卵管中的表达与调节。mPGES-2 蛋白主要定位于不同时期的伞部和壶腹部的上皮纤毛细胞中，在伞部和壶腹部肌层以及峡部和宫管连接处的上皮和肌层中未检测到mPGES-2 蛋白的表达。

On the basis of light microscopic observation the immunohistochemical examination of CK,TG,NSE,vimentin and calcitonin was used to observe 9 cases of oxyphilic cell adenoma of the thyroid.

观察9例甲状腺嗜酸细胞腺瘤，在光镜观察的基础上，用免疫组织化学方法检测细胞角蛋白、甲状腺球蛋白、神经元特异性烯醇化酶、波形蛋白和降钙素。

The expression status of 〓 andRB protein in the tissues of normal, chronic pancreatitisand pancreatogenic carcinoma were detected by immunohis-tochmical staining. Also, the relationship be-tween the feature of cancer and prognosis was analysed in combi-nation with clinical data.

采用免疫组织化学染色，检查胰腺癌、慢性胰腺炎、正常胰腺等组织的〓蛋白和RB蛋白表达状态，并结合临床资料，将其与胰腺癌的生物学特性及予后的关系加以分析。

Methods: By reverse transcriptase polymerase chain reaction and immunohisto-chemical assay, the expression of Surviving gene and bcl-2 protein were detected in 16 cases normal tissue of bladder, 12 cases papillate tumor and 59 cases transitional cell cancer of bladder.

采用逆转录PCR方法检测了16例正常膀胱组织、12例膀胱移行细胞乳头状瘤和59例膀胱TCC组织Survivin基因的表达，同时用免疫组化学方法检测以上组织中bcl-2蛋白的表达，并将结果进行相关分析。

Zymography test and immunohistochemical technique were used separately to determine the activity of MMP-2 and MMP-9 of the paraffine sample and the refrigeration sample.

石蜡标本进行免疫组织化学定位检测MMP-2、MMP-9，冷冻标本用Zymography方法进行MMP-2、MMP-9活性测定。

The section covers metabolic, membrane, receptor and immunological disorders as well as viral, bacterial and parasitological diseases, and includes the biochemistry of differentiation disorders, tissue damage and aging.

其内容涵盖代谢，生物膜，受体和免疫失调，病毒、细菌和寄生虫疾病、各种失调、组织损伤以及与老化有关的生物化学等。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。