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免疫化学

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Childhood mesenteric and omental IMT is a rare interstitial tumor. The diagnosis should be based on pathological and immunohistochemistry examinations.

儿童肠系膜及网膜IMT是一种较为罕见的间叶性肿瘤,诊断必须依靠病理学检查和免疫组织化学检测。

Methods Male Sprague Dawley rats were induced by ligating the anterior descending coronary artery and were divided into the 2nd, 4th, 8th, 12th and 21st week groups. Elastic fibers were evaluated in the infarct region using Masson trichrome staining, Uana orcein staining and image analysis. The expressions of TNFα and IL-1β were also detected by immunohistochemistry.

SD大鼠冠状动脉前降支结扎形成心肌梗死模型,于术后2、4、8、12、21周时,用Masson染色、Uana地衣红染色技术和免疫组织化学方法,分别观察梗死区弹性纤维和白细胞介素1β(IL-1β)、肿瘤坏死因子的变化,计算机图像分析系统作半定量分析。

Correlations between the above indexes and between the above indexes and the degree of ossicular destruction were investigated.

采用免疫组织化学技术SABC法检测CDK4、P16在中耳胆脂瘤35例、正常外耳道皮肤20例中的表达,并结合胆脂瘤对听小骨骨质破坏程度,采用SPSS11.5软件进行统计学分析。

Methods vWF protein was examined using immunohis-tochemical SABC staining method in 54 cases of osteosarcoma and 10 cases of osteochondroma.

方法应用免疫组织化学SABC方法检测10例骨软骨瘤和54例骨肉瘤组织中的vWF表达情况。

Methods PTEN and Caspase-3 were detected by S-P immunohistochemical method in 38 cases of paraffin-embedded section of osteosarcoma and 20 of osteochondroma.

方法用免疫组织化学方法检测PTEN和Caspase-3在38例骨肉瘤及20例骨软骨瘤中的表达。

Methods Expression of livin protein were detected by immunohistochemistry staining assay in 45 osteosarcoma cases and 30 osteochondroma cases.

采用免疫组织化学SABC法检测45例骨肉瘤、30例骨软骨瘤凋亡抑制蛋白Livin表达,并分析其与临床病理因素的关系。

Methods Skp2 protein and p27 protein were examined by using immunohistochemical SP staining method in 48 cases of osteosarcoma and 16 cases of osteochondroma.

用免疫组织化学SP法检测16例骨软骨瘤和48例骨肉瘤组织中Skp2和p27蛋白的表达情况。

Results Negative expression of Skp2 was detected in all osteochondroma. In contrast, it was detected in 43.75%(21/48)of osteosarcoma.

用免疫组织化学SP法检测16例骨软骨瘤和48例骨肉瘤组织中Skp2和p27蛋白的表达情况。

Methods: The immunohistochemistry was used to investigate the protein expression of p27 in 46 cases of osteosarcoma and 16 cases of osteochondroma. The expression of p27 and histological category and differentiation levels of osteosarcoma were observed.

应用S-P免疫组织化学方法检测46例骨肉瘤和16例骨软骨瘤组织中p27的表达,并观察p27表达在骨肉瘤组织学分型、分化程度等临床指标之间的差异。

Methods: Immunohistochemistry was used to detect the expressions of PTTG protein and c-myc protein in 37 osteosarcoma and 12 osteoenchondroma.

采用免疫组织化学的方法及图像分析技术检测37例骨肉瘤和12例骨软骨瘤中PTTG、c-myc蛋白的表达。并与骨肉瘤主要临床病理学参数及转移进行比较。

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