免疫化学

In addition of cytomorphologic identification, immunolhistochemic technique of bone morphogenic protein-monoclonal antibody, alkaline Phosphatase staining and concentration assays, as well as osteoblasts reaction to PTH-cAMP were used, according to the biologic character of osteoblasts.

除了在细胞形态学上对其鉴定外，还根据成骨细胞的生物学特性，首次引用了骨形成蛋白单克隆抗体免疫细胞化学技术，以及碱性磷酸酶染色和含量测定，成骨细胞对甲状旁腺激素-cAMP反应等方法，验证了经酶消化分离培养的兔颅骨细胞具有成骨细胞的功能和特性。

Methods Immunohistochemical and morphometrical analysis methods were performed on vital skin wounds. Results In normal and injury mouse skin IL-10 immunoreactivity was observed in epidermal cells.

方法应用免疫组织化学技术和形态计量法，对小鼠不同时程皮肤切创组织中IL-10不同表达部位（表皮细胞及表皮下组织的浸润细胞）的变化进行研究。

Objective To study the histological and immunohistochemical features of primary and metastatic ovarian mucinous adenocarcinoma.

目的 探讨卵巢原发性黏液性腺癌与转移性黏液性腺癌的组织学和免疫组织化学特点。

Using histology, immunohistochemistry and electron microscopy, we studied the distribution and morphological structure of the thyroid follicle grey mullet, Mugil cephalus.

中文摘要：用组织学、免疫组织化学和电镜技术对鲻鱼甲状腺的分布与形态结构进行研究。

Using histology, immunohistochemistry and electron microsco py, we studied the distribution and morphological structure of the thyroid folli cle grey mu llet, Mugil cephalus.

用组织学、免疫组织化学和电镜技术对鲻鱼甲状腺的分布与形态结构进行研究。

Some researchers have found that SP could affect other neurons in enteric nervous system . However, little is known about the mechanism of SP on the myenteric cholinergic neurons.

本研究采用全细胞膜片钳技术和免疫细胞化学技术探讨了SP对豚鼠回肠肌间丛胆碱能神经元电活动的影响，为揭示肽类递质对胆碱能神经元的调节机制提供依据。

METHODS: Thirty male SD rats were randomly divided into control group, stress group and NG-nitro-L-arginine methyl ester group. The rat model of water immersion-restraint stress was established. The expression of nNOS in colonic submucous plexus and myenteric plexus in the rats was examined by immuno-histochemical staining and analyzed by computer image analysis system.

SD大鼠30只随机分为对照组，应激组和L-NAME组，采用水浸-束缚应激动物模型，用免疫组织化学ABC法检测nNOS在大鼠结肠黏膜下神经丛和肌间神经丛的表达，应用计算机图像分析系统对其表达进行定量分析。

C2C12 cells were induced to differentiation into muscle cells in vitro and the myogenesis model was detected by inverted microscope, RT-PCR, western-blot, cell immunofluorescence histochemistry, flow cytometry and confirmed to be successful. On the foundation of differentiation model, miR-206 was detected by northern hybridization and the result showed that the expression level increasing gradually during muscle differentiation with a character of phase specificity.

利用C2C12细胞在体外成功诱导其向成肌细胞分化，通过形态学观察，RT-PCR、western-blot、细胞免疫荧光组织化学及流式细胞仪等方法进行检测，证实我们所建立的细胞分化模型是成功的，并观察到随着诱导时间的延长，miR-206在成肌分化过程中表达量逐渐增高的，表达改变具有阶段特异性。

To study the role of FoxO1 on myoblast proliferation and differentiation and the transformation of muscle fiber type in porcine,here we use yorkshire as experimental materials and detect the expression of genes related myogenesis and muscle fiber type in pporcine myoblast that was knockout or activated FoxO1,adopting the technology of RNAi,real time-PCR and Western blotting et al..

为探讨FoxO1对猪成肌细胞增殖分化及肌纤维类型转化的作用及机理，本试验以大白猪为研究材料，利用RNAi、real-time PCR、免疫细胞化学和Western blotting等技术，通过在原代培养的猪成肌细胞中敲除和激活内源活性FoxO1，检测肌肉形成与肌纤维类型相关基因的表达。

Methods 124 DFSPs were reviewed by light microscopy. 6 cases with areas of myoid/myofibroblastic differentiation were assayed with immunohisto-chemical technique and electron microscopy was applied in two cases.

采用常规HE切片对124例DFSP进行筛选，对6例伴有肌样／肌纤维母细胞性分化的DFSP病例进行免疫组织化学标记，其中2例加做电镜检测。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。