免疫化学

Methods: The expressions of the neurexin protein and neuroligin protein in the myenteric nerve plexus in 20 cases with congenital megacolon were detected by immunohistochemistry.

将20例先天性巨结肠狭窄段、移行段和扩张段制备成肌间神经丛铺片标本，采用免疫组织化学技术，对neurexin、neuroligin蛋白进行检测。

Serum estradiol level of rats was detected by means of radioimmunoassay. The positive staining and intensity of lipid in the meibomian gland alveolus was detected by Sudan Ⅲ staining.

实验评估：采用放射免疫分析方法检测血清雌二醇水平；采用组织化学苏丹Ⅲ染色方法对睑板腺腺泡上皮做脂质染色阳性和强度检测。

Immunohistochemistry was used to mensurate COX-2 expression in endometrium and uterus muscle layer of two groups.

采用免疫组织化学方法检测COX-2在两组子宫内膜和肌组织中的表达。

Methods In both malformed embryos induced by excess retinoid acid and normal KM mice,expressions of Dishevelled2 and Vangl2 in the epithelium of the palatal shelves and the mesenchyma were determined by immunohistochemistry and image analysis.

方法采用免疫组织化学及图像分析方法，检测维甲酸致畸小鼠和相应对照组小鼠腭板中Dishevelled2和Vangl2蛋白的表达状况。

In the past, the origin of these tumors was thought to be mesothelial cells.

在过去认为这种肿瘤是由肋膜生长出，但藉由免疫组织化学染色技术的进步，我们了解事实上孤立性肋膜纤维瘤是由肋膜下肺间叶所生长出之肿瘤。

The central part was composed of hypertrophic chondrocytes, for which the number increased with time. The cartilaginous tissue at the time of 2 weeks' cultivation appeared strongly positive staining of collagen type Ⅱ immunohistochemistry and safranine "O", as well as strongly metachromatic to toluidine blue.

其中心为肥大软骨细胞，且随培养时间的延长而增多。2周时，软骨组织甲苯胺蓝染色呈强的红色异染反应，Ⅱ型胶原免疫组织化学染色呈强阳性，番红&O&染色呈强阳性。

Methods: The expressions of CGRP and VEGF in the wound tissue were detected by immunohistochmisty technique and MIAS image analysis system.

应用免疫组织化学方法、图像分析技术动态检测大鼠创伤组织中CGRP，VEGF的表达。

METHODS: 8-Bromo-cAMP and sodium nitroprusside were added into the media to raise the concentrations of cAMP and cGMP of cultured cardiomyocytes, in situ hybridization and immunohistochemistry methods were used to identify the mRNA and protein expression of bFGF. The amount of bFGF mRNA and protein expression were detected by computor imaging analysis system MIAS exe.

分离培养大鼠心肌细胞，加入8-溴-cAMP和硝普钠，升高细胞内cAMP、cGMP浓度，用原位杂交及免疫细胞化学的方法观察细胞内bFGF的表达，图象分析检测bFGF表达量的变化。

To observe the influence of tumor necrosis factora on differentiation of rat mesencephalic neural stem cells,the numbers of neurons,astrocytes and oligodendrocytes generated from NSCs were analyzed after differentiation for 3 days by using i m munocytoche mistry technique.

为观察肿瘤坏死因子对神经干细胞分化的影响，本研究应用体外扩增的新生大鼠中脑NSCs，使用免疫组织化学技术，观察了肿瘤坏死因子一。对NSC、分化及其后代细胞的影响。

METHODS: NSCs were isolated from neonatal rats by enzyme digestion and mechanical separation. At the fourth passage, cell clone masses received nestin immunocytochemistry. Remaining cells were dispersed by mechanical separation. Monoclone NSCs were incubated by limiting dilution assay, and made into 108 L-1 monoplast suspension in complete medium. NSCs were assigned into 2 groups. NSCs in the control group were incubated in 10% fetal bovine serum.

酶消化和机械分离法相结合体外分离培养新生鼠神经干细胞，传至第4代的细胞克隆团行巢蛋白免疫细胞化学染色观察，剩余细胞团用机械法分散，采用有限稀释法进行单克隆神经干细胞培养，加入完全培养基制成108 L-1的单细胞悬液，分为2组滴入培养板，对照组加入10%FBS，诱导组加入10%FBS+50 μg/L神经生长因子，培养5~7 d。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。