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免疫化学

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Immunohistochemistry was utilized to investigate the light and electron micros- copic localization of neurotensin-like immunoreactive amacrine cells in the chicken retina.

本实验用光镜和电镜免疫组织化学方法研究神经紧张素(neurotensin,NT)样免疫反应无长突细胞在鸡视网膜的定位。

Histology, immunohistochemistry and electron microscope were used to study the structures and functions of the endostyle of amphioxus and the thyroid gland of grey mullet.

免疫组织化学结果揭示,内柱第3和第5区带细胞的顶端胞质及纤毛对鼠抗人甲状腺球蛋白抗体呈免疫阳性反应。

The expressions of HO-1 and HO-2 mRNA were detected using reverse transcription polymerase chain and the relative amount of CO released into the media was quantitated as carboxyhemoglobin by enzyme linked immunosorbent assay.

通过逆转录聚合酶链式反应和免疫细胞化学法检测HO-1、HO-2 mRNA和蛋白的表达,酶联免疫法检测培养上清液中碳氧血红蛋白含量,四唑氮比色法检测细胞增殖。

Purpose In the present study, the protective effect of SOD on the paraquat -induced inflammatory injury in hamster cheek pouch mucosa was assessed. Furthermore. a series of experiments were performed to study the mechanisms of SOD anti-inflammatory action in this study.

(1)通过常规组织病理学观察、图像分析系统等技术,评价SOD防治金黄地鼠口腔粘膜炎性损害的效果;(2)应用电子自旋共振、免疫组织化学、放射免疫法等技术,探讨SOD可能的抗炎机理。

Experimental methods: pathological slices: To observe the changes of pathomorphology and measure the circular area of internal elastic lamina , area of neonate intima and vascular lumen, the intimal proliferatory index and percentage, the index of lumen stenosis and positive index of proliferating cell nuclear antigen through the specimens stained by HE and immunohistochemistry.

实验方法:病理切片:进行HE染色和免疫组织化学染色,分别观察病理形态学改变;测定和计算受损血管内弹力膜环绕总面积、新生内膜面积、管腔面积、内膜增生指数、内膜增生百分比、管腔狭窄指数和增殖细胞核抗原阳性指数;观察MMP-2、MMP-3、TIMP-1和PDGF-BB的免疫组化表达。

Methods The distribution of the calcitonin gene-related peptide-immunoreactive fibers in the cremaster arteries had been studied by means of ABC technique.Results The calcitonin gene-related peptide-immunoreactive fibers were observed on all branches of cremaster arteries.

方法采用免疫组织化学ABC法对大白鼠提睾肌动脉各级分支的CGRP免疫反应阳性纤维的分布特点进行观察和研究。

Both the histopathologic and immunohistochemical studies show that LP is the T lym- phocyte-mediated immunity reaction, but it's etiology has remained obscure.

组织病理学和免疫组织化学的研究均表明LP损害是T细介导的免疫反应,但诱发该免疫反应的病因至今尚不明确。

Using immuno-histochemical, tissue cultural and immuno-electron microscopic methods, based on nerve-endocrine-immune net, the dynamic changes of 4 brain-gut peptides and 6 inflammation factors of excised saccalus rotunds of rabbit stimulated by germs, LPS, exotoxin were observed.

本研究以神经-内分泌-免疫网络学说为依据,运用免疫组织化学的方法,结合组织培养技术、免疫电镜技术等,对离体兔圆小囊受到大肠杆菌等4种细菌、内毒素、外毒素等抗原刺激后,其组织内的4种脑肠肽和6种炎症相关因子表达量的动态变化进行了系统观察研究。

No immunostaining was seen in RPE. Double staining that Kir2. 1 and glutamine synthetase, Kir2. 1 and glutamine synthetase colocalized well. Intense Kir7. 1 immunolabeling was present on the apical surface of all RPE cells and appeared to extend over the length of the apical processes. Na〓, K〓-ATPase expression varied among RPE cells, but in highly expressing cells, it co-localized with Kir7. 1. Immunoreactivity of Kir2. 1、Kir4. 1 and Kir7. 1 acomplished by ABC immunohistochemistry in monkey and human retinae got the nearly same results as bovine.

间接免疫荧光组织化学显示,Kir2.1蛋白主要分布在Müller细胞与神经元相接触的细胞膜区域;与Müller细胞的特异性标记蛋白质抗谷氨酸合成酶抗体双重标记显示其分布特性重叠,在RPE未检测Kir2.1蛋白的免疫活性存在;Kir4.1蛋白集中分布于Müller细胞的内侧突起,与GS蛋白双重标记显示其分布特性重叠,RPE未检测Kir4.1蛋白的免疫活性存在;Kir7.1主要分布于RPE游离面及其突起的全长,与特异性标记RPE突起的Ezrin蛋白完全重叠,Na〓-K〓ATP酶在不同部位的RPE表达不同,Na〓-K〓ATP高表达的RPE细胞与Kir7.1的分布特性重叠。

The lumbar spinal cord in normal rat was immunohistochemically stained with ABC method using HAP1B antibody. Under light microscope, it was observed that HAP1 immunoreactants is mainly distributed in the pericaryon and neuropil in superficial lamella of the gray nucleus with little or no expression in other layers of the spinal horn. The substantia alba show no HAP1B immunoreactants.

对正常大鼠腰段脊髓进行HAP1B的ABC法免疫组织化学染色,光镜下观察显示,HAP1免疫反应产物主要分布在脊髓灰质背角浅层神经元胞体和神经毡内,灰质其他各层无或极少HAP1B表达,白质内未见明显HAP1B免疫反应产物。

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