免疫化学

Methods:The subcutaneous adipose tissue was obtained from the fold inguen of four SD rats, then digested with collagenase type Ⅰ and cultured in endothelial medium for seven days.

获取4只雄性SD大鼠鼠蹊部皮下脂肪，通过机械分割和组织消化法获取脂肪基质细胞，在内皮细胞培养液中培养7d后，经免疫细胞化学及透射电镜鉴定证实95%以上细胞已具内皮细胞表型特征，再将这些细胞进行成脂(DMEM/F-12培养基内加入0.5mmol/L1-甲基-3-异丁基-黄嘌呤，1μmol/L地塞米松，10μmol/L胰岛素，200μmol/L吲哚美星)定向诱导7d，形态学观察和油红O特殊染色鉴定诱导后的细胞。

In order to distinguish the type of GFP positive cells in glomeruli further, we also carried out fluorescence histochemistry double staining, the results of it were: the GFP positive cells in recipients glomeruli in mice of the four, five, six groups were blood cells in glomeruli capillary loop, but not the glomeruli resident cells, for example, endothelial cells, epithelial cells or intercapillary cells.

为了进一步鉴别受体鼠肾小球内GFP阳性细胞的种类，我们进行了免疫荧光组织化学双染色，结果显示：④、⑤、⑥组受体鼠肾小球内的GFP阳性细胞可能是肾小球毛细血管袢内的血细胞而非肾小球的固有细胞，即内皮细胞、上皮细胞或系膜细胞。

We introduced improved primary mixed glial culture and different-attachment method to isolate and purify the OPCs, the cells were proliferated in serum-free medium, flow cytometry and immunohischemistry methods were employed to estimate the purity of cultured OPCs. Their abilities of differentiation and expression of trophic factors were identified by RT-PCR and immunostaining. Several methods including TUNEL and MTT were adopted to estimate the protective effects of conditioned culture medium from oligodendrocyte lineage cells on the primary cultured cerebellar granule neurons. Intravitreal transplant of OPCs, combined with retrograde fluorescent labeling the superior colliculus and intraorbital optic nerve transection, were used to investigate the protective effects of OPCs on the axotomized RGCs in vivo. Intravitreal transplantof OPCs or NSCs on the newborn rats, and retinal transplant of OPCs on the young rats were performed, to observe the myelin formation in the retina at different stages after cellular transplantation. Optic nerve transection was carried out on some rats with myelinated retinae, to study the influence of myelination on the injuried RGCs.

为此，本研究采用改良的胶质细胞混合培养与差速贴壁方法获得大鼠OPCs，使用无血清培养基进行扩增、培养，用免疫组织化学和流式细胞技术对培养细胞的纯度进行鉴定，对少突胶质系细胞表达部分营养因子的情况进行检测；采用TUNEL、MTT等方法对少突胶质系细胞条件培养基对原代培养小脑颗粒神经元的保护作用进行检测；将OPCs移植入成年SD大鼠玻璃体内，利用上丘逆行荧光标记技术，观察眼内移植的OPCs对眶内视神经切断时的视网膜神经节细胞的保护作用及其持续时间；将OPCs或NSCs移植入新生和幼年SD大鼠玻璃体或视网膜内，观察不同时期视网膜内髓鞘形成与分布特点，分析髓鞘的超微结构，并观察眼内髓鞘形成对损伤神经节细胞的保护作用。

Methods Five groups (n=24) of SD rats were randomly assigned to received intrapleural injection of dexamethasone, dipopolysaccharide, erythromycin, hypertonic saline and normal saline, respectively. The AQP1 protein in pleural was detected with immunohistochemistry. The mRNA expression of AQP1 under stimulations at different time points was measured by real time RT-PCR.

SD大鼠按实验设计分为5组(n=24)：地塞米松组、内毒素组、红霉素组、高渗盐水组及对照组，通过免疫组织化学染色测定AQP1在胸膜上的表达和定位；采用Real-time RT-PCR方法对不同刺激因子作用下不同时间胸膜组织AQP1的基因表达进行定量分析。

The Sprague-Dawley rats were intratracheally instilled with lipopolysaccharide(LPS,2 mg·kg-1 per day) for two days to induce acute lung injury.The rats were sacrificed at 72 hours after LPS instillation.Lung morphology was studied.

气管内注入LPS复制大鼠急性肺损伤模型，观察72 h后的病理变化、支气管肺泡灌洗液中细胞总数的变化、免疫组织化学特点以及蓝桉油的影响。

MAIN OUTCOME MEASURES: Lacunae changes of osteocytes in femoral heads were detected with hematoxylin-eosin staining method, and the expression of Bcl-2 and Bax proteins with immunohistochemistry staining methods, after 3, 6, 12, 24, 48 and 96 hours of ischemia respectively.

主要观察指标：应用苏木精-伊红染色，免疫组织化学来观测股骨头缺血后3，6，12，24，48，96h股骨头内骨细胞陷窝变化及Bcl-2、Bax蛋白表达的变化情况。

Methods Specific endothelial cell markers with immunohistochemistry and microvessel density with morphometry in 28 casas of laryngopharyngeal malignant melanoma.

方法应用免疫组织化学染色法检测28例咽喉部恶性黑色素瘤中血管内皮细胞特异性标记物Ⅷ因子相关抗原（factor FⅧ-related antigen， FⅧ-RAg）和CD31的表达，并通过形态学计量法计数肿瘤内微血管密度。

By using the immunohistochemistry method of Strept Avidin-Biotin-Complex, four kinds of antisera raised against rabbit hormones: somatostatin, pancreatic polypeptide, 5-hydroxytryptamine （ 5-HT ） and neuropeptide Y were used to indentify and locate the endocrine cells in the digestive tract of Sparus latus.

应用4种兔抗胃肠激素抗体和SABC免疫组织化学方法，对黄鳍鲷消化道中的内分泌细胞进行鉴别和定位。

Was simultaneously injected into cisterna magna at the second injection blood in the presence of subarachnoid blood. At 2 to 4 days, GFP was expressed in leptomeninges over the brain stem, cortex and cerebral arteries, smooth muscle cells of small vessels were occasionlly transduced. GFP was expressed in adventitia of spastic basilar artery on days 2 (day 5 after first injection blood) after injection 〓, but was undetectable by days 4, transgene was not expressed in medial or intimal layers. The prensence of subarachnoid blood can not prevent access of adenovirus to vessels and transgene expression.

注入腺病毒载体后2天（初次注血第5天）行荧光显微镜、免疫组织化学和RT-PCR检测，结果在颅内大血管如基底动脉的外膜可见外源基因的表达，而外源基因不能转移至血管中膜和内膜，4天时（初次注血第7天）基底动脉的外膜中外源基因表达消失；注入腺病毒载体后2～4天，外源基因可以有效转移至颅底软脑膜细胞，小血管外膜和平滑肌层也可见外源基因的表达，表明蛛网膜下腔内的血凝块不能阻止腺病毒载体介导外源基因转移至颅内血管。

The Immunohistochemical results indicated that the pI〓 is obviously located on the liminal surface membrane of the distal tubules and Henle's ascending limbs, and can also be found at Henle's loop of porcine kidneys, these segments expose in non-isotonic condition.

免疫组织化学染色显示，pI〓在猪肾脏明显定位于非等渗区域的远曲小管和髓袢升支粗段的管腔表面膜及髓袢处，而等渗区域的近曲小管细胞几乎无pI〓的存在，据此认为pI〓可能在耐受渗透压的变化上发挥作用。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。