免疫化学

Immunocytochemistry method was used to demonstrate the expression of glial fibrillary acidic protein.

用塑料吸头划刮培养皿，制造机械损伤的模型，继续培养至72 h，分别取出不同时间点的细胞进行胶质细胞增生情况的观察和免疫组织化学染色，对胶质原纤维酸性蛋白表达产物光密度进行图像分析。

Methods The expression of survivin and Ki-67 in 80 cases of breast carcinoma, 10 cases of fibroadenoma and 10 cases of normal breast tissue were detected and analyzed by immunohistochemistry and computerized image.

方法采用免疫组织化学SP法及图像分析技术检测和分析80例乳腺癌、10例乳腺纤维腺瘤和10例正常乳腺组织中生存素、Ki67的表达。

MethodsThe tissue microarray and immunohistochemistry SP methods were used to detect the MMP-13 expression in 97 cases of breast cancer and 39 cases of breast fibroadenoma and its correlation with clinicopathologic features was analyzed.

应用组织芯片技术和免疫组织化学SP法检测MMP-13在97例乳腺癌和39例乳腺纤维腺瘤中的表达情况，并分析其与临床病理参数的关系。

Methods The expressions of Rb protein were detected in 52 patients with breast cancer, 20 patients with breast fibroadenoma or breast cystic hyperplasia by S-P immunohistochemical method.

采用S-P免疫组织化学染色法对52例乳腺癌组织和20例良性乳腺病变组织中Rb蛋白的表达进行检测。

Methods Nuclear β-catenin expression was detected by immunohistochemistry in 77 lesions with desmoid-type fibromatosis and 171 other spindle cell lesions, including superficial fibrmoatosis (n=18), nodular fasciitis (n=36), keloid (n=16), scar (n=10), granulation tissue (n=9), synovial sarcoma (n=38), neufibroma (n=13), solitary fibrous tumor (n=12), gastrointestinal stromal tumor (n=10), low-grade myxofibrosarcoma (n=3), low-grade fibromyxoid sarcoma (n=3), and smooth muscle tumor (n=10). In addition, the immunohistochemical expressions of ER-α, ER-β and Ki-67 were examined in all of the lesions with desmoid-type fibromatosis. The nuclear immunohistochemical staining for nuclear βcatenin and ER-β was graded as high level (≥25% of cells), low level (5%-25%) or none.

采用免疫组织化学染色法检测77例韧带样型纤维瘤病和171例其它良、恶性梭形细胞病变（包括结节性筋膜炎36例、浅部纤维瘤病11例、瘢痕疙瘩16例、增生性瘢痕10例、肉芽组织9例、滑膜肉瘤38例、神经纤维瘤13例、孤立性纤维性肿瘤12例、胃肠间质肿瘤10例、低度恶性黏液纤维肉瘤3例、低度恶性纤维黏液样肉瘤3例及平滑肌肿瘤10例）组织中β-catenin核阳性的表达，同时检测韧带样型纤维瘤病中ER-α、ER-β和Ki-67的表达。

Methods We examined the E-cad expression in 16 cases of carcinoma of endometrium and 10 cases of fibromyoma or ovarian cyst as control by immunohistochemistry.

采用免疫组织化学方法检测 16例子宫内膜癌及 10例子宫肌瘤、卵巢囊肿患者的E cad表达。结果子宫内膜癌患者的E cad表达阳性率较对照组显著增高(P 。0 5 )。

METHODS: The presence of MMP-2 and MMP-9 was investigated in 20 fibrovascular epiretinal membranes of PDR, and the findings were compared with those observed in 10 avascular epiretinal membranes of proliferative vitreoretinopathy.

采用免疫组织化学方法，检测PDR患者20例视网膜前纤维血管膜MMP-2和MMP-9的表达，并与增殖性玻璃体视网膜病变（proliferative vitreoretinopathy ，PVR）10例非血管性增殖膜进行对比研究。

Immunohistochemistry assays were applied to detect the protein expression of MMP-9 and PAI-1 in arteriovenous fistular vascular segments.

通过免疫组织化学技术，在蛋白质水平检测36例动静脉内瘘血管内皮细胞中的MMP-9和 PAI-1的变化。

In this research, the pubertal five-week-old SD rats were chose as experimental object to establish the animal model for simulating maxillary protraction, on which was imposed the orthopedic force of 85g with utilizing the self-devised maxillary protraction appliance. Located lateral X-ray cephalograms were taken to measure and analyze the changes of maxillary development after exerting the orthopedic force for 4 weeks. The technique of immunohistochemistry was used to investigate theexpression of TGF-, in frontomaxillary sutures and palatomaxillary sutures with identical force performing for different extent of time. The average hue was selected as indicator, which was measured by the means of the analysis system of pathological color images. The measuring data were analyzed by the one-way ANOVA in software SPSS 11.0 version.

本研究采用自行设计的上颌前牵引装置，以生长发育期5周龄SD大鼠为实验对象，施加85g的矫形力，建立了模拟上颌前牵引的动物模型，通过X线头影测量分析，观察施力4周后上颌骨的生长发育情况；应用免疫组织化学技术，检测同一力值作用不同时段下TGF-β_1在上颌骨的额颌缝、腭颌缝的表达，彩色病理图象分析系统测定每张切片中阳性染色细胞的平均灰度值，结果采用SPSS 11.0进行方差分析。

After the culture for 14 days, the proliferated fusiform cells began to increase in a great amount.

诱导后第9天，收集细胞，行MTT比色法、流式细胞仪和免疫组织化学检测鉴定细胞。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。